A5064562948- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Advanced Proteomics Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- DNA and Nucleic Acid Chemistry
- Synthesis and Characterization of Heterocyclic Compounds
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
Goethe University Frankfurt
2017-2020
Significance ABC transporters shuttle chemically diverse substances across membranes in an energy-dependent manner. They mediate multidrug resistance microorganisms and cancer cells can cause human pathologies when dysfunctional. Although important insights into have been gained recent years, fundamental questions concerning their mechanism remain open. Here, we identify the protein complex TmrAB as a functional homolog of antigenic peptide transporter TAP present its high-resolution...
Abstract Polyacrylamide gel electrophoresis (PAGE) and immunoblotting (Western blotting) are the most common methods in life science. In conjunction with these methods, polyhistidine-tag has proven to be a superb fusion tag for protein purification as well specific detection by immunoblotting, which led vast amount of commercially available antibodies. Nevertheless, antibody batch-to-batch variations nonspecific binding complicate laborious procedure. The interaction principle applied...