A5024203904- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Photoreceptor and optogenetics research
- Radiopharmaceutical Chemistry and Applications
- Biochemical and Structural Characterization
- Transgenic Plants and Applications
- Fluorine in Organic Chemistry
- Advanced Biosensing Techniques and Applications
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Chemical Reactions and Isotopes
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Biotin and Related Studies
- Neuroscience and Neural Engineering
- Nanofabrication and Lithography Techniques
- Neuroscience and Neuropharmacology Research
Goethe University Frankfurt
2018-2023
University of Würzburg
2023
Abstract Microbial rhodopsins are photoreceptor proteins that convert light into biological signals or energy. Proteins of the xanthorhodopsin family common in eukaryotic photosynthetic plankton including diatoms. However, their role these organisms remains elusive. Here we report on a variant ( Fc R1) isolated from polar diatom Fragilariopsis cylindrus . Applying combination biophysical, biochemical and reverse genetics approaches, demonstrate R1 is plastid-localized proton pump which binds...
Using engineered nanobodies with bright organic dyes (fluorescent nanobodies) and subsequent microfluidic cell manipulation, controlled nanobody delivery was achieved, allowing the multiplexed imaging super-resolution of endogenous protein networks in living cells.
Small chemical/biological interaction pairs are at the forefront in tracing protein function and high signal-to-background ratios cellular pathways. However, optimal design of scaffold, linker, chelator head still deserve systematic investigation to achieve highest affinity kinetic stability for vitro especially applications. We report on a library N-nitrilotriacetic acid (NTA)-based multivalent heads (MCHs) built linear, cyclic, dendritic scaffolds compare these with regard their binding...
Due to their high stability and specificity in living cells, fluorescently labeled nanobodies are perfect probes for visualizing intracellular targets at an endogenous level. However, intrabodies bind unrestrainedly hence may interfere with the target protein function. Here, we report a strategy prevent premature binding through development of photo-conditional intrabodies. Using genetic code expansion, introduce photocaged amino acids within nanobody-binding interface, which, after...
Abstract Live‐cell labeling, super‐resolution microscopy, single‐molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of function is essential to derive unbiased insights into cellular processes. Herein, we define a new class hexavalent N ‐nitrilotriacetic acid ( hexa NTA) chelators, displaying the highest affinity stability all NTA‐based pairs described so far....
Genetic code expansion is a versatile method for in situ synthesis of modified proteins. During mRNA translation, amber stop codons are suppressed to site-specifically incorporate non-canonical amino acids. Thus, nanobodies can be equipped with photocaged acids control target binding on demand. The efficiency suppression and protein vary unpredictable background expression, the reasons hardly understood. Here, we identified substantial limitation that prevented N-terminal modifications light...
Abstract Polyacrylamide gel electrophoresis (PAGE) and immunoblotting (Western blotting) are the most common methods in life science. In conjunction with these methods, polyhistidine-tag has proven to be a superb fusion tag for protein purification as well specific detection by immunoblotting, which led vast amount of commercially available antibodies. Nevertheless, antibody batch-to-batch variations nonspecific binding complicate laborious procedure. The interaction principle applied...
Abstract Small chemical/biological interaction pairs are at the forefront in tracing protein function and high signal‐to‐background ratios cellular pathways. However, optimal design of scaffold, linker, chelator head still deserve systematic investigation to achieve highest affinity kinetic stability for vitro especially applications. We report on a library N ‐nitrilotriacetic acid (NTA)‐based multivalent heads (MCHs) built linear, cyclic, dendritic scaffolds compare these with regard their...
Abstract Live‐cell labeling, super‐resolution microscopy, single‐molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of function is essential to derive unbiased insights into cellular processes. Herein, we define a new class hexavalent N ‐nitrilotriacetic acid ( hexa NTA) chelators, displaying the highest affinity stability all NTA‐based pairs described so far....
TrisNTA-Chelatoren bestehen aus drei metallkomplexierenden N-Nitrilotriessigsäure(NTA)-Derivaten, die über eine Gerüststruktur verbunden sind. Gekoppelt an einen Fluorophor oder anderen Reporter können trisNTAs als Sonden für Kennzeichnung histidinmarkierter Proteine in lebenden Zellen dienen. R. Tampé et al. berichten ihrer Zuschrift auf S. 12575 große Affinitäts- und Stabilitätsunterschiede, durch lineare, dendritische cyclische Gerüste hervorgerufen werden, sie klären, welche...
Abstract ChR2-XXL and GtACR1 are currently the cation anion ends of optogenetic single channel current range. These were used in primary rat cortical neurons vitro to manipulate neuronal firing patterns. provides high currents via elevated light sensitivity a prolonged open state. Stimulating expressing putative presynaptic induced neurotransmission. Moreover, stable depolarisation block could be generated using ChR2-XXL, proving that is promising candidate for vivo applications...
Fluoreszenzsonden In ihrer Zuschrift auf S. 5722 berichten R. Tampé et al. über eine neue Klasse hexavalenter Chelatoren, welche die Auswahl an selektiven und hochaffinen Markierungspaaren vergrößert. Diese Sonden können Informationen dynamische zelluläre Prozesse liefern.