A5049471662- Legionella and Acanthamoeba research
- Research on Leishmaniasis Studies
- Bacterial biofilms and quorum sensing
- Heme Oxygenase-1 and Carbon Monoxide
- Trypanosoma species research and implications
- Insect Pest Control Strategies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ocular Infections and Treatments
- Vibrio bacteria research studies
- Ocular Surface and Contact Lens
- Synthesis and Biological Evaluation
- Water Treatment and Disinfection
- Essential Oils and Antimicrobial Activity
- Phytochemicals and Medicinal Plants
- Rabies epidemiology and control
- Bacillus and Francisella bacterial research
- Parasites and Host Interactions
- Bioactive Compounds and Antitumor Agents
- Phytochemical compounds biological activities
- Papaya Research and Applications
- Phytochemistry and Biological Activities
- Synthesis and biological activity
- Natural Compounds in Disease Treatment
- Malaria Research and Control
- Cynara cardunculus studies
Instituto de Salud Carlos III
2020-2025
Centro de Investigación Biomédica en Red
2021-2025
Universidad de La Laguna
2016-2025
Hospital Universitario de Canarias
2010-2024
Universidad Europea de Canarias
2015-2019
University of Edinburgh
2017
Écologie et Biologie des Interactions
2017
Université de Poitiers
2017
Centre National de la Recherche Scientifique
2017
ABSTRACT Members of the genus Acanthamoeba are facultative pathogens humans, causing a sight-threatening keratitis and life-threatening encephalitis. In order to treat those infections properly, it is necessary target treatment not only trophozoite but also cyst. Furthermore, may be advantageous avoid parasite killing by necrosis, which induce local inflammation. We must toxicity host tissue. Many drugs eukaryotes known programmed cell death (PCD), this process poorly characterized in ....
Chagas disease, caused by the protozoan Trypanosoma cruzi, affects millions globally, with limited treatment options available. Current therapies, such as benznidazole and nifurtimox, present challenges, including their toxicity, side effects, inefficacy in chronic phase. This study explores potential of drug repurposing a strategy to identify new treatments for T. focusing on compounds from Medicines Malaria Venture (MMV) COVID Box. An initial screening 160 identified eight trypanocidal...
Free-living amoebae (FLA) include opportunistic pathogens such as Naegleria fowleri, Balamuthia mandrillaris, and the genera Sappinia Acanthamoeba. In this study, a survey was conducted in order to evaluate presence of potentially pathogenic amoebic strains water samples collected from wells located western part Guinea-Bissau. The were left precipitate for 48 hours then sediments seeded on non-nutrient agar plates containing Escherichia coli spread cultures checked daily FLA. Identification...
Chagas disease and leishmaniasis are neglected tropical diseases caused by kinetoplastid parasites of Trypanosoma Leishmania genera that affect poor remote populations in developing countries. These share similar complex life cycles modes infection. It has been demonstrated the particular group phosphorylating enzymes, protein kinases (PKs), essential for infective mechanisms parasite survival. The natural indolocarbazole staurosporine (STS, 1) extensively used as a PKC inhibitor its...
Leishmaniasis, produced by Leishmania spp., and Chagas disease, Trypanosoma cruzi, affect millions of people around the world. The treatments for these pathologies are not entirely effective produce some side effects. For reasons, it is necessary to develop new therapies that more active less toxic patients. Some initiatives, such as one carried out Medicines Malaria Venture, allow screening a large number compounds different origins find alternatives lack trypanocide treatments. In this...
ABSTRACT Pathogenic strains of the genus Acanthamoeba are causative agents severe infections, such as fatal encephalitis and a sight-threatening amoebic keratitis. Antimicrobial therapy for these infections is generally empirical, patient recovery often problematic, due to existence highly resistant cyst stage in amoebae. In previous studies, small interfering RNAs (siRNAs) against catalytic domains extracellular serine proteases glycogen phosphorylase from were designed evaluated future...
ABSTRACT Acanthamoeba is an opportunistic pathogen in humans, whose infections most commonly manifest as keratitis or, more rarely, granulomatous amoebic encephalitis. Although there are many therapeutic options for the treatment of , they generally lengthy and/or have limited efficacy. Therefore, a requirement identification, validation, and development novel targets against these pathogens. Recently, RNA interference (RNAi) has been widely used validation purposes proven to be powerful...
Therapy against Acanthamoeba infections such as Granulomatous Amoebic Encephalitis (GAE) and Keratitis (AK), remains an issue to be solved due the existence of a cyst stage which is highly resistant most chemical physical agents. Recently, activity Olive Leaf Extracts (OLE) was demonstrated species. However, molecules involved in this were not identified and/or evaluated. Therefore, aim study evaluate main are present OLE secondly their mechanism action Acanthamoeba. Among tested molecules,...
Recently, the search for novel therapeutic agents against Acanthamoeba species has been focused on evaluation of natural resources. Among them, marine microorganisms have risen as a source bioactive compounds with advantage ability to obtain unlimited and constant amounts in contrast other sources such plants. Furthermore, actinomycetes recently reported highly rich including salinosporamides, xiamycines, indolocarbazoles, naphtyridines, phenols, dilactones antimycines macrolides among...
Naegleria fowleri causes a deadly disease called primary amoebic meningoencephalitis (PAM). Even though PAM is still considered rare disease, the number of reported cases worldwide has been increasing each year. Among factors to be for this, awareness about this and also global warming, as these amoebae thrive in warm water bodies, seem key factors. Until present, no fully effective drugs have developed treat PAM, current options are amphotericin B miltefosine, which present side effects...
Neglected tropical diseases such as leishmaniasis and American trypanosomiasis represent an increasing health problem. Current treatments are not satisfactory which remains urgent need for novel, cheap safe chemotherapies. In the course of our ongoing search new potential anti-protozoal agents, this study aimed to perform a bio-guided fractionation Inula viscosa (Asteraceae) using in vitro assays against three strains Leishmania Trypanosma genus. Eight known compounds were identified from...
Free living, cosmopolitan amoebae from Acanthamoeba genus present a serious risk to human health. As facultative parasites, these may cause keratitis (AK). is severe, vision-threatening corneal infection with non-specific symptoms. The number of reported AK cases worldwide has been increasing every year. Moreover, 90% are related contact lens use. Wearing and storage lenses not in accordance the physicians manufacturers recommendations primary key factors this disease. Amoebae can easily...
Leishmaniasis and Chagas disease, two of the most prevalent neglected tropical diseases, are a world health problem. The harsh reality these infective diseases is absence effective safe therapies. In this framework, natural products play an important role in overcoming current need to development new antiparasitic agents. present study reports synthesis, antikinetoplastid screening, mechanism fourteen withaferin A derivatives (2-15). Nine them (2-6, 8-10 12) showed potent dose-dependent...
Acanthamoeba spp. are opportunistic pathogens that ubiquitous in nature. Many species of this genus responsible for a fatal encephalitis and keratitis humans other animals. Seventy-two soil samples were collected from the parishes across Jamaica assessed presence Cultivation was carried out on non-nutrient agar plates seeded with heat killed Escherichia coli. PCR sequencing DF3 region order to genotype isolated strains Acanthamoeba. Thermotolerance osmotolerance assays utilized investigate...