A5035167285- Fungal Infections and Studies
- Coastal wetland ecosystem dynamics
- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Constructed Wetlands for Wastewater Treatment
- Gene Regulatory Network Analysis
- Gut microbiota and health
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Marine and coastal plant biology
- Advanced Fluorescence Microscopy Techniques
- Biological Control of Invasive Species
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Microbial Natural Products and Biosynthesis
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmaceutical and Antibiotic Environmental Impacts
University of Toronto
2018-2024
Fungal pathogens pose a global threat to human health, with Candida albicans among the leading killers. Systematic analysis of essential genes provides powerful strategy discover potential antifungal targets. Here, we build machine learning model generate genome-wide gene essentiality predictions for C. and expand largest functional genomics resource in this pathogen (the GRACE collection) by 866 genes. Using chemogenomic analyses, define function three uncharacterized roles kinetochore...
ABSTRACT Microbiomes often benefit plants, conferring resistance to pathogens, improving stress tolerance, or promoting plant growth. As potential mutualists, however, microbiomes are not a single organism but community of species with complex interactions among microbial taxa and between microbes their shared host. The nature ecological in the microbiome can have important consequences for net effects on hosts. Here, we compared individual strains 10-strain synthetic communities...
Stochastic signaling dynamics expand living cells' information processing capabilities. An increasing number of studies report that regulators encode in their pulsatile dynamics. The evolutionary mechanisms lead to complex remain uncharacterized, perhaps because key interactions proteins are encoded intrinsically disordered regions (IDRs), whose evolution is difficult analyze. Here we focused on the IDR controls stochastic pulsing Crz1, a transcription factor fungi downstream widely...
At human body temperature, the fungal pathogen
Abstract Microbiomes often benefit plants, conferring resistance to pathogens, improving stress tolerance, or promoting plant growth. As potential mutualists, however, microbiomes are not a single organism but community of species with complex interactions among microbial taxa and between microbes their shared host. The nature ecological in the microbiome can have important consequences for net effects on hosts. Here, we compared individual strains 10-strain synthetic communities...
The ability to transition between yeast and filamentous growth states is critical for virulence of the leading human fungal pathogen Candida albicans. Large-scale genetic screens have identified hundreds genes required this morphological switch, but mechanisms by which many these orchestrate developmental remain largely elusive. In study, we characterized role Ent2 in governing morphogenesis C. We showed that under a wide range inducing conditions also mouse model systemic candidiasis. found...
Abstract The picture emerging from the rapidly growing literature on host-associated micro-biota is that host traits and fitness often depend complex interactive effects of genotype, microbial interactions, abiotic environment. However, testing these main typically requires large, multi-factorial experiments thus remains challenging in many systems. Furthermore, most studies plant microbiomes focus terrestrial hosts microbes. Aquatic habitats may confer unique properties to micriobiomes. We...
Abstract Stochastic signaling dynamics expand living cells’ information processing capabilities. An increasing number of studies report that regulators encode in their pulsatile dynamics. The evolutionary mechanisms lead to complex remain uncharacterized, perhaps because key interactions proteins are encoded intrinsically disordered regions (IDRs), whose evolution is difficult analyze. Here we focused on the stochastic pulsing Crz1, a transcription factor fungi downstream widely conserved...
Candida albicans is the leading cause of systemic candidiasis. Effective treatment threatened by a dearth antifungal options and emergence resistance. Thus, there an urgent need to identify novel therapeutic targets expand our armamentarium. A promising approach discovery essential genes, as most antimicrobials target bioprocesses. Despite detailed characterization gene essentiality in Saccharomyces cerevisiae,defining pathogen interest necessary due high level divergence between these...