A5100368872- RNA and protein synthesis mechanisms
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Signaling Pathways in Disease
- Fungal Infections and Studies
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Genetics, Bioinformatics, and Biomedical Research
- Enzyme Structure and Function
- Pancreatic and Hepatic Oncology Research
- Microbial Natural Products and Biosynthesis
- Biotin and Related Studies
- Pancreatitis Pathology and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Gene expression and cancer classification
- Machine Learning in Bioinformatics
- Gut microbiota and health
- Biochemical and Molecular Research
University of Minnesota
2010-2025
Beijing Academy of Agricultural and Forestry Sciences
2024
Twin Cities Orthopedics
2023
Chongqing Medical University
2019
Cornell University
2010
Quest Diagnostics (United States)
2010
Columbia University
2003
Abstract Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal strong genetic dependency between major N-terminal acetyltransferase and specific ubiquitin ligases. Biochemical analyses uncover that both ligase complex UBR4-KCMF1 NatC recognize bearing an unacetylated methionine followed by hydrophobic residue. KO-induced protein degradation phenotypes reversed...
Fungal pathogens pose a global threat to human health, with Candida albicans among the leading killers. Systematic analysis of essential genes provides powerful strategy discover potential antifungal targets. Here, we build machine learning model generate genome-wide gene essentiality predictions for C. and expand largest functional genomics resource in this pathogen (the GRACE collection) by 866 genes. Using chemogenomic analyses, define function three uncharacterized roles kinetochore...
Fungal pathogens such as Candida albicans pose a significant threat to human health with limited treatment options available. One strategy expand the therapeutic target space is identify genes important for pathogen growth in host-relevant environments. Here, we leverage pooled functional genomic screening fitness of C. diverse conditions. We an essential gene no known Saccharomyces cerevisiae homolog, C1_09670C, and demonstrate that it encodes subunit 3 replication factor A (Rfa3)....
Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research enhancing agricultural traits. In this study, we tested PRIME-Del (PDel) strategy using a pair prime editing guide RNAs (pegRNAs) that targeted opposite DNA strands achieved an average efficiency 55.8% 60 bp fragment deletions at six endogenous targets. Moreover, as high 84.2% was obtained 2000
ABSTRACT Decoding the language of DNA sequences is a fundamental problem in genome research. Mainstream pre-trained models like DNABERT-2 and Nucleotide Transformer have demonstrated remarkable achievements across spectrum analysis tasks. Yet, these still face pivotal challenge (1) genetic diversity, or capability to capture variations individuals populations foundation models; (2) model efficiency, specifically how enhance performance at scalable costs for large-scale foundational (3)...
SUMMARY Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal strong genetic dependency between major N-terminal acetyltransferase and specific ubiquitin ligases. Biochemical analyses uncover that both ligase complex UBR4-KCMF1 NatC recognize bearing an unacetylated methionine followed by hydrophobic residue. KO-induced protein degradation phenotypes reversed...
In recent years a number of de novo sequencing software products became available providing possible partial or complete amino acid sequence tags for MS/MS spectra peptides.However, variety reasons including spectral chemical noise and imperfect fragmentation these almost always contain errors.Additional difficulties arise from actual protein variation post-translational modifications.We present search engine named PepTiger which is capable correctly matching with errors to sequences in...
Data in this article are associated with the research "High-throughput screening of enzyme mutants by comparison their activity ratios to an tag" (Li et al., 2019) [1]. provided on development a system for high-throughput (HTP) through applicable and its suitable tag cell lysates fused forms, Escherichia coli alkaline phosphatase (ECAP) as N-terminus Pseudomonas Aeruginosa arylsulfatase (PAAS) via flexible linker. were made publicly available further analyses.
Candida albicans is the leading cause of systemic candidiasis. Effective treatment threatened by a dearth antifungal options and emergence resistance. Thus, there an urgent need to identify novel therapeutic targets expand our armamentarium. A promising approach discovery essential genes, as most antimicrobials target bioprocesses. Despite detailed characterization gene essentiality in Saccharomyces cerevisiae,defining pathogen interest necessary due high level divergence between these...