A5045892870- Protein Interaction Studies and Fluorescence Analysis
- Bacteriophages and microbial interactions
- Nanoparticle-Based Drug Delivery
- Hemoglobin structure and function
- Animal Virus Infections Studies
- Spectroscopy and Quantum Chemical Studies
- Plant Virus Research Studies
- Mass Spectrometry Techniques and Applications
- Polymer Surface Interaction Studies
- Protein Structure and Dynamics
- Advanced Proteomics Techniques and Applications
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Proteins in Food Systems
- RNA Interference and Gene Delivery
- Characterization and Applications of Magnetic Nanoparticles
- Nanomaterials and Printing Technologies
- Molecular Biology Techniques and Applications
- Protein Hydrolysis and Bioactive Peptides
- Advanced Electron Microscopy Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Diatoms and Algae Research
- Alzheimer's disease research and treatments
- Bacterial biofilms and quorum sensing
- Blood properties and coagulation
Université Paris-Saclay
2016-2024
Centre National de la Recherche Scientifique
2016-2024
CEA Paris-Saclay
2016-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2024
Laboratoire des Matériaux et du Génie Physique
2021-2023
Institut polytechnique de Grenoble
2021-2023
Laboratoire de physique des Solides
2020-2022
Infection et inflammation
2019-2021
Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux
2018-2020
Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Énergie
2018-2020
The coffee-ring effect denotes the accumulation of particles at edge an evaporating sessile drop pinned on a substrate. Because it can be detected by simple visual inspection, this ubiquitous phenomenon envisioned as robust and cost-effective diagnostic tool. Toward direction, here we systematically analyze deposit morphology drying drops containing polystyrene different surface properties with various proteins (bovine serum albumin (BSA) forms hemoglobin). We show that patterns reveal...
Upon contact with biological fluids, nanoparticles (NPs) are readily coated by cellular compounds, particularly proteins, which determining factors for the localization and toxicity of NPs in organism. Here, we improved a methodological approach to identify proteins that adsorb on silica high affinity. Using large-scale proteomics mixtures soluble prepared either from yeast cells or alveolar human cells, observed large unstructured region(s) more prone bind NPs. These disordered regions...
Previous self-assembly experiments on a model icosahedral plant virus have shown that, under physiological conditions, capsid proteins initially bind to the genome through an en masse mechanism and form nucleoprotein complexes in disordered state, which raises question as how virions are assembled into highly ordered structure host cell. Using small-angle X-ray scattering, we find out that disorder–order transition occurs conditions upon increase protein concentrations. Our cryo-transmission...
Few experimental techniques allow the analysis of protein corona in situ. As a result, little is known on effects nanoparticles weakly bound proteins that form soft corona. Despite its biological importance, our understanding molecular bases driving formation limited. Here, we show hemoglobin can either hard or silica depending pH conditions. Using cryoTEM and synchrotron-radiation circular dichroism, alter structure stability Molecular dynamics simulation identified structural elements...
Protein adsorption on a surface is generally evaluated in terms of the evolution proteins' structures and functions. However, when that nanoparticle, protein corona formed around it possesses particular supramolecular structure gives "biological identity" to new object. Little known about actual shape corona. Here, by model proteins (myoglobin hemoglobin) silica nanoparticles was studied. Small-angle neutron scattering oxygenation studies were combined assess both structural functional...
Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that corona drives biological distribution toxicity of NPs. Here, composition structure were studied using silica nanoparticles (SiNPs) different sizes interacting with soluble yeast extracts. Adsorption isotherms showed amount adsorbed proteins varied greatly upon NP size large NPs having more per surface unit. The was a large-scale label-free proteomic approach,...
Protein adsorption on nanoparticles is an important field of study, particularly with regard to nanomedicine and nanotoxicology. Many factors can influence the composition structure layer(s) adsorbed proteins, so-called protein corona. However, role size has not been specifically investigated, although some evidence indicated its potential in corona structure. To assess size, we studied interactions hemoproteins (spanning a large range) monodisperse silica nanoparticles. We combined various...
Bone strength is an important factor for determining fracture risk that dependent on bone mass and architecture as well material properties. properties are defined, in part, by the heterogeneity degree of mineralization, parameters can be assessed with a mineral density distribution (BMDD) from 2D-histological sections using backscatter electron microscopy (BSEM). Here we perform comprehensive examination 3D-μCT-based BMDD to assess its utility research. Analysis cortical μCT scans...
Noble metal materials, especially platinum nanoparticles (Pt NPs), have immense potential in nanomedicine as therapeutic agents on account of their high electron density and surface area. Intravenous injection is proposed the best mode to deliver product patients. However, our understanding reaction with blood components, proteins, far behind explosive development these agents. Using synchrotron radiation circular dichroism (SRCD), we investigated structural stability changes human serum...
The gadolinium-based nanoagent named AGuIX® is a unique radiosensitizer and contrast agent which improves the performance of radiotherapy medical imaging. Currently tested in clinical trials, administrated to patients via intravenous injection. presence nanoparticles blood stream may induce harmful effects due undesired interactions with components. Thus, there an emerging need understand impact these nanoagents when meeting proteins. In this work, influence on structure stability most...
Albumin forms a complex with the squalene-adenosine prodrug and by doing so drives disassembly of nanoparticles.
The adsorption of proteins on surfaces has been studied for a long time, but the relationship between structural and functional properties adsorbed protein mechanism remains unclear. Using hemoglobin silica nanoparticles, we have previously shown that hemoglobin's affinity towards oxygen increases with adsorption. Nevertheless, it was also there were no significant changes in quaternary secondary structures. In order to understand change activity, decided this work focus active sites...
Understanding the mechanisms involved in interaction of proteins with inorganic surfaces is major interest for both basic research and practical applications involving nanotechnology. From list cellular highest affinity silica nanoparticles, we highlighted group containing arginine–glycine–glycine (RGG) motifs. Biochemical experiments confirmed that RGG motifs interact strongly surfaces. The these further increased when R residue asymmetrically, but not symmetrically, dimethylated. Molecular...
X-ray photoelectron spectroscopy of bovine serum albumin (BSA) in a liquid jet is used to investigate the electronic structure solvated protein, yielding insight into charge transfer mechanisms biological systems their natural environment. No structural damage was observed BSA following sample Carbon and nitrogen atoms different chemical environments were resolved spectra both solid BSA. The calculations distributions demonstrate difficulty assigning contributions complex an aqueous...
Previous self-assembly experiments on a model icosahedral plant virus have shown that, under physiological conditions, capsid proteins initially bind to the genome through an en masse mechanism and form nucleoprotein complexes in disordered state, which raises questions as how virions are assembled into highly ordered structure host cell. Using small-angle X-ray scattering, we find out that disorder-order transition occurs conditions upon increase protein concentrations. Our...