A5065014248- Marine Biology and Ecology Research
- Aquatic Invertebrate Ecology and Behavior
- Advanced Glycation End Products research
- Marine Bivalve and Aquaculture Studies
- Protist diversity and phylogeny
- Redox biology and oxidative stress
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Hemoglobin structure and function
- Endoplasmic Reticulum Stress and Disease
- Biochemical effects in animals
- Microtubule and mitosis dynamics
- Insect symbiosis and bacterial influences
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- 14-3-3 protein interactions
- RNA and protein synthesis mechanisms
- Parasite Biology and Host Interactions
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Legionella and Acanthamoeba research
- Bioactive Compounds and Antitumor Agents
- Sulfur Compounds in Biology
Centre National de la Recherche Scientifique
2009-2024
Sorbonne Université
2009-2024
Station Biologique de Roscoff
2009-2024
Adaptation et Diversité en Milieu Marin
2010-2022
Université Paris Cité
2001-2015
Délégation Paris 7
2004-2012
Institut Gustave Roussy
1999
ESPCI Paris
1996
Peptide methionine sulphoxide reductase (PMSR, EC 1.8.4.6), the msrA or pmsR gene product, is a ubiquitous enzyme catalysing reduction of to in proteins. Decreased expression and/or activity PMSR with age could explain, at least part, accumulation oxidized protein observed upon aging. To test this hypothesis, rat cDNA was cloned and sequenced. The recombinant expressed, its catalytic checked synthetic substrate polyclonal antibodies were raised against PMSR. as well then analysed function...
Proteins are sensitive to reactive oxygen species, and the accumulation of oxidized proteins has been implicated in aging process other age-related pathologies. In proteins, methionine residues especially oxidation, leading S - R -methionine sulphoxide diastereoisomers, reversion which is achieved by peptide reductases MsrA MsrB respectively. The enzyme, addition its role repair, forms part species scavenging systems that important cellular antioxidant defence. present most living organisms,...
Protein adsorption on nanoparticles is an important field of study, particularly with regard to nanomedicine and nanotoxicology. Many factors can influence the composition structure layer(s) adsorbed proteins, so-called protein corona. However, role size has not been specifically investigated, although some evidence indicated its potential in corona structure. To assess size, we studied interactions hemoproteins (spanning a large range) monodisperse silica nanoparticles. We combined various...
Temperature, perhaps more than any other environmental factor, is likely to influence the evolution of all organisms. It also a very interesting factor understand how genomes are shaped by selection over evolutionary timescales, as it potentially affects whole genome. Among thermophilic prokaryotes, temperature both codon usage and protein composition increase stability transcriptional/translational machinery, resulting proteins need be functional at high temperatures. eukaryotes less known...
Abstract Background Alvinella pompejana is a representative of Annelids, key phylum for evo-devo studies that still poorly studied at the sequence level. A. inhabits deep-sea hydrothermal vents and currently known as one most thermotolerant Eukaryotes in marine environments, withstanding largest chemical thermal ranges (from 5 to 105°C). This tube-dwelling worm forms dense colonies on surface chimneys can withstand long periods hypo/anoxia phases exposure hydrogen sulphides. specifically...
Taking advantage of the massive genome sequencing effort made on thermophilic prokaryotes, thermal adaptation has been extensively studied by analysing amino acid replacements and codon usage in these unicellular organisms. In most cases, to thermophily is associated with greater residue hydrophobicity more charged residues. Both characteristics are positively correlated optimal growth temperature prokaryotes. contrast, little information collected molecular 'adaptive' strategy eukaryotes....
After proteolytic digestion of sperm tubulin from sea urchin Paracentrotus lividus, C-terminal peptides were isolated by chromatographic separations. The analyzed Edman degradation and matrix-assisted laser desorption/ionization-time flight mass spectrometry. About 70% the unmodified. remaining modified have undergone a combination numerous posttranslational modifications generating significant heterogeneity tubulin. α-Tubulin is detyrosylation, release penultimate glutamate,...
Several types of post‐translational modifications contribute to the high level tubulin heterogeneity in brain. An important modification is glutamylation major brain‐specific isotypes, such as class Ia/b α‐tubulin and classes II III β‐tubulin. Here we describe experiments determine if additional, minor expressed adult mouse brain, could also be glutamylated. Purified from brain was cleaved with thermolysin. Proteolytically released carboxy‐terminal peptides both α‐ β‐tubulin were isolated by...
Abstract Here we report the discovery of a high-temperature hydrothermal vent field on Woodlark Ridge, using ship-borne multibeam echosounding and Remotely Operated Vehicle (ROV) exploration. La Scala Vent Field comprises two main active areas several inactive zones dominated by variably altered basaltic rocks, indicating that an stable circulation has been maintained over long period time. The Pandora Site, at depth 3380 m, is mainly composed diffuse vents. Corto site, 3360 characterized...
Abstract Molluscs have undergone many transitions between separate sexes and hermaphroditism, which is of interest in studying the evolution sex determination differentiation. Here, we combined multi-locus genotypes obtained from restriction site-associated DNA (RAD) sequencing with anatomical observations gonads three deep-sea hydrothermal vent gastropods genus Alviniconcha living southwest Pacific. We found that all species (Alviniconcha boucheti, strummeri, kojimai) share same...
Aging is accompanied by gradual cellular dysfunction associated with an accumulation of damaged proteins, particularly via oxidative processes. This has been attributed, at least in part, to impairment mitochondrial function as this organelle both a major source oxidants and target for their damaging effects, which can result reduction energy production, thereby compromising cell function. In the present study, we observed significant decrease respiratory activity rat liver mitochondria...
Deep hydrothermal vents are highly fragmented and unstable habitats at all temporal spatial scales. Such environmental dynamics likely play a non-negligible role in speciation. Little is, however, known about the evolutionary processes that drive population-level differentiation vent species isolation and, more specifically, how geography habitat specialisation interplay history of divergence. In this study, range divergence Alviniconcha snails occupy active Western Pacific fields was...
Colonization of deep-sea hydrothermal vents by most invertebrates was made efficient through their adaptation to a symbiotic lifestyle with chemosynthetic bacteria, the primary producers in these ecosystems. Anatomical adaptations such as establishment specialized cells or organs have been evidenced numerous invertebrates. However, very few studies detailed global inter-dependencies between host and symbionts In this study, we proposed describe, using proteo-transcriptomic approach, effects...
The aim of this study was first to identify lysozymes paralogs in the deep sea mussel Bathymodiolus azoricus then measure their relative expression or activity different tissue conditions. B. is a bivalve that lives close hydrothermal chimney Mid-Atlantic Ridge (MAR). They harbour specialized gill cells two types endosymbiont (gram—bacteria): sulphide oxidizing bacteria (SOX) and methanotrophic (MOX). This association thought be ruled by specific mechanism actors regulation deal with...
Alvinella pompejana is one of the most emblematic species animal communities colonizing deep-sea hydrothermal vents East Pacific Rise. This extreme environment characterized by high temporal variability its physical-chemical parameters. Among these, variation in concentration available oxygen should lead to a specific physiological adaptive response animal. To evaluate mechanisms this at molecular level, classical 2-DE-based proteomic approach has been implemented. After collection...
Abstract: A combination of posttranslational modifications contributes to the high heterogeneity brain tubulin in mammals. In this report, structures detyrosinated carboxy‐terminal peptides α‐tubulin from newborn and adult mouse were compared. The these was observed increase tubulin. major part increased is due excision Glu 450 , which makes nontyrosinatable (Δ‐2 tubulin). polyglutamyl side chain bi‐ triglutamylated analyzed work. polyglutamylation α‐tubulin, first glutamyl residue can only...
A bstract : Oxidatively modified proteins have been analyzed in aging human peripheral blood lymphocytes since protein modification by oxidation and other related pathways are believed to contribute the intracellular age‐related accumulation of damaged proteins, a process that has associated with cellular functional deficits occur age. Advanced glycation end products (AGE) were quantified pattern glycated two‐dimensional gel electrophoresis followed Western blotting using an anti‐AGE...
Multi-domain proteins form the majority of in eukaryotes. During their formation by tandem duplication or gene fusion, new interactions between domains may arise as a result structurally-forced proximity domains. The proper function formed likely required molecular adjustment these stress zones specific amino acid replacements, which should be detectable signature selection that governed changes. We used multi-domain globins from three different invertebrate lineages to investigate selective...