A5072631153- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Estrogen and related hormone effects
- Allergic Rhinitis and Sensitization
- Microfluidic and Capillary Electrophoresis Applications
- Cerebrovascular and genetic disorders
- Microbial Metabolic Engineering and Bioproduction
- Food Allergy and Anaphylaxis Research
- Coenzyme Q10 studies and effects
- Effects of Radiation Exposure
- Biotin and Related Studies
- Glycogen Storage Diseases and Myoclonus
- Bioinformatics and Genomic Networks
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Electrolyte and hormonal disorders
- Heat shock proteins research
- Molecular Biology Techniques and Applications
- Cardiac Valve Diseases and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Occupational exposure and asthma
- Porphyrin Metabolism and Disorders
- NF-κB Signaling Pathways
- Bioactive Compounds and Antitumor Agents
- Protein Tyrosine Phosphatases
ESPCI Paris
2008-2025
Centre National de la Recherche Scientifique
2008-2025
Université Paris Sciences et Lettres
2016-2025
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2013-2024
Laboratoire Plasticité du Cerveau
2023-2024
ParisTech
2010-2019
Odense University Hospital
2017
Chimie ParisTech
2013
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, or CADASIL, one of the most common inherited small vessel diseases brain, is characterized by a progressive loss vascular smooth muscle cells extracellular matrix accumulation. The disease caused highly stereotyped mutations within domain NOTCH3 receptor (Notch3ECD) that result in an odd number cysteine residues. While CADASIL-associated differentially affect function activity, they all are associated...
The ChipFilter device is suited to prepare microorganism samples for parallel proteomic and genomic analyses, which particularly relevant in the case of low-abundant drastically reduces sampling bias.
The Drosophila defense against pathogens largely relies on the activation of two signaling pathways: immune deficiency (IMD) and Toll. IMD pathway is triggered mainly by Gram-negative bacteria, whereas Toll responds predominantly to Gram-positive bacteria fungi. these pathways leads rapid induction numerous NF-κB-induced response genes, including antimicrobial peptide genes. shows significant similarities with TNF receptor pathway. Recent evidence indicates that also activated in various...
Abstract Italian cypress ( Cupressus sempervirens , Cups ) pollen causes allergic diseases in inhabitants of many the cities surrounding Mediterranean basin. However, allergens are still poorly known. We introduce here a novel proteomic approach based on double one‐dimensional gel electrophoresis (D1‐DE) as an alternative to 2‐DE immunoblot, for specific IgE screening allergenic proteins from extracts. The sequential combination IEF and SDS‐PAGE associated with immunoblotting allows...
Brain glycogen and its metabolism are increasingly recognized as major players in brain functions. Moreover, alteration of the contributes to neurodegenerative processes. In brain, both muscle phosphorylase isozymes regulate mobilization. However, given their distinct regulatory features, these two could confer metabolic functions brain. Interestingly, recent proteomics studies have identified isozyme-specific reactive cysteine residues (bGP). this study, we show that activity human bGP is...
The finding of new diagnostic and prognostic markers local radiation injury, particularly the cutaneous syndrome, is crucial for its medical management, in case both accidental exposure radiotherapy side effects. Especially, a fast high-throughput method still needed triage people accidentally exposed to ionizing radiation. In this study, we investigated impact localized irradiation skin on early alteration serum proteome mice an effort discover associated with severity impending damage....
Colonization of deep-sea hydrothermal vents by most invertebrates was made efficient through their adaptation to a symbiotic lifestyle with chemosynthetic bacteria, the primary producers in these ecosystems. Anatomical adaptations such as establishment specialized cells or organs have been evidenced numerous invertebrates. However, very few studies detailed global inter-dependencies between host and symbionts In this study, we proposed describe, using proteo-transcriptomic approach, effects...
The metaproteomic approach is an attractive way to describe a microbiome at the functional level, allowing identification and quantification of proteins across broad dynamic range as well detection post-translational modifications. However, it remains relatively underutilized, mainly due technical challenges that should be addressed, including complexity extracting from heterogeneous microbial communities. Here, we show ChipFilter microfluidic device coupled liquid chromatography tandem mass...
Peptide tagging is a useful tool to improve matrix-assisted laser desorption/ionization tandem mass spectrometric (MALDI-MS/MS) analysis. We present new application of the use dansyl chloride (DNS-Cl). DNS-Cl specific primary amine reagent widely used in protein biochemistry. It adds fluorescent dimethylaminonaphthalene moiety molecule. The evaluation MALDI-MS and MS/MS analyses dansylated peptides shows that dansylation raises ionization efficiency most hydrophilic species compared with...
The way in which estrogen receptor alpha (ERalpha) mediates gene transcription and hormone-dependent cancer cell proliferation is now being largely reconsidered view of several recent discoveries. ERalpha-mediated appears to be a cyclic transient process where the proteasome - thus degradation plays pivotal role. In our investigations, demonstrate estrogenic activity synthetic peptide corresponding regulatory motif (ERalpha17p), we propose that ERalpha proteasomal could induce emergence...
Peanut, soybean, sesame and lentil are members of legumes worldwide consumed by human that can induce food allergy in genetically predisposed individuals. Several protein allergens, mainly water-soluble, have been described. We studied the non water-soluble fraction from these 4 sources using immunoproteomics tools techniques. Flour extracts were solubilized detergent chaotropes analysed 1 2 dimensional gel electrophoresis (2D). Results showed numerous proteins exhibiting wide ranges...
Combining different "omics" approaches, such as genomics and proteomics, is necessary to generate a detailed complete insight into microbiome comprehension. Proper sample collection processing accurate analytical methods are crucial in generating reliable data. We previously developed the ChipFilter device for proteomic analysis of microbial samples. have shown that this coupled LC-MS/MS can successfully be used identify proteins. In present work, we our workflow analyze concomitantly...
ABSTRACT Metaproteomic approach is an attractive way to describe a microbiome at the functional level, allowing identification and quantification of proteins across broad dynamic range as well detection post-translational modifications. However, it remains relatively underutilized, mainly due technical challenges that should be addressed, including complexity in extracting from heterogenous microbial communities. Here, we show ChipFilter microfluidic device coupled LC-MS/MS can successfully...
For many years, HUVEC.com1 public database provides biological data relative to the proteome of human umbilical vein endothelial cells (HU-VECs), which are most used cell model in vascular biology. The proteins were identified using two-dimensional gel electrophoresis (2-DGE) for protein separation coupled with Matrix Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI-TOF-MS) identification. We present here an important update HUVEC.com 521 identifications as determined Fourier...
Background In a recent proteomic study, we identified in Italian cypress (Cupressus sempervirens, Cups) pollen grains, 2 proteins at 43 and 60 kDa, homologous to already known Cupressaceae polygalacturonase (PG) proteins. The 60-kDa PG is suspected be multi-protein complex including the 43-kDa one or more with lectin-like properties