A5030939574- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Chromosomal and Genetic Variations
- Aquatic Invertebrate Ecology and Behavior
- Evolution and Genetic Dynamics
- Insect Pest Control Strategies
- Insect-Plant Interactions and Control
- Genome Rearrangement Algorithms
- Invertebrate Immune Response Mechanisms
- Hemoglobin structure and function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect behavior and control techniques
- Marine Bivalve and Aquaculture Studies
- Entomopathogenic Microorganisms in Pest Control
- Protist diversity and phylogeny
- Algorithms and Data Compression
- Insect Utilization and Effects
- Plant pathogens and resistance mechanisms
- Environmental DNA in Biodiversity Studies
- Insect and Pesticide Research
- Forensic and Genetic Research
- Genetic and Environmental Crop Studies
Pennsylvania State University
2014-2024
John Wiley & Sons (United States)
2016
Hudson Institute
2016
University of California, Davis
2016
Advanced Bioscience Laboratories (United States)
2016
Waters (United States)
2005
University of Massachusetts Chan Medical School
2005
University of Georgia
1986-2003
Centre Hospitalier Universitaire de Caen
1999
Hôpital de l'Antiquaille
1999
We have sequenced the genome of a second Drosophila species, pseudoobscura , and compared this to sequence melanogaster primary model organism. Throughout evolution vast majority genes remained on same chromosome arm, but within each arm gene order has been extensively reshuffled, leading minimum 921 syntenic blocks shared between species. A repetitive is found in D. at many junctions adjacent blocks. Analysis novel element family suggests that recombination offset elements may given rise...
To advance our understanding of adaptation to temporally varying selection pressures, we identified signatures seasonal occurring in parallel among Drosophila melanogaster populations. Specifically, estimated allele frequencies genome-wide from flies sampled early and late the growing season 20 widely dispersed We frequency shifts across North America Europe, demonstrating that is a general phenomenon temperate fly Seasonally fluctuating polymorphisms are enriched large chromosomal...
Abstract The sequencing of the 12 genomes members genus Drosophila was taken as an opportunity to reevaluate genetic and physical maps for 11 species, in part aid mapping assembled scaffolds. Here, we present overview importance cytogenetic biology concepts chromosomal evolution. Physical markers were used anchor genome assembly scaffolds polytene each species. In addition, a computational approach smaller on basis analysis syntenic blocks. We map data from sequenced non-Drosophila...
Abstract The availability of 12 complete genomes various species genus Drosophila provides a unique opportunity to analyze genome-scale chromosomal rearrangements among group closely related species. This article reports on the comparison gene order between these and fixed rearrangement events that disrupt order. Three major themes are addressed: conservation syntenic blocks across species, disruption (via inversion events) its relationship phylogenetic distribution rate over evolutionary...
Understanding the role of chromosomal inversions in speciation is a fundamental problem evolutionary genetics. Here, we perform comprehensive reconstruction histories Drosophila persimilis and D. pseudoobscura. We provide solution to puzzling origins selfish Sex-Ratio arrangement uncover surprising patterns phylogenetic discordance on this chromosome. These show that, contrary widely held views, all fixed between pseudoobscura were already present their ancestral population long before...
Abstract Drosophila melanogaster is a leading model in population genetics and genomics, growing number of whole-genome data sets from natural populations this species have been published over the last years. A major challenge integration disparate sets, often generated using different sequencing technologies bioinformatic pipelines, which hampers our ability to address questions about evolution species. Here we these issues by developing bioinformatics pipeline that maps pooled (Pool-Seq)...
In Escherichia coli, Saccharomyces cerevisiae, and Drosophila melanogaster, codon bias may be maintained by a balance among mutation pressure, genetic drift, natural selection favoring translationally superior codons. Under such an evolutionary model, silent mutations fall into two fitness categories: preferred that increase unpreferred changes in the opposite direction. This prediction can tested comparing frequency spectra of synonymous segregating within populations; will elevate...
Abstract As whole-genome sequence assemblies accumulate, a challenge is to determine how these can be used address fundamental evolutionary questions, such as inferring the process of speciation. Here, we use Drosophila pseudoobscura and D. persimilis test hypotheses regarding divergence with gene flow. We observe low differentiation between two genome sequences in pericentromeric peritelomeric regions. interpret this result primarily remnant correlation levels variation local recombination...
The gene arrangements of Drosophila have played a prominent role in the history evolutionary biology from original quantification genetic diversity to current studies mechanisms for origin and establishment new inversion mutations within populations their subsequent fixation between species supporting reproductive barriers. This review examines causes consequences inversions as recombination suppressors that suppression plays establishing they are involved adaptation heterogeneous...
Chromosomal rearrangements can shape the structure of genetic variation in genome directly through alteration genes at breakpoints or indirectly by holding combinations variants together due to reduced recombination. The third chromosome Drosophila pseudoobscura is a model system test hypotheses about how are established populations because its polymorphic for >30 gene arrangements that were generated series overlapping inversion mutations. Circumstantial evidence has suggested these...
Drosophila pseudoobscura harbors a rich polymorphism for paracentric inversions on the third chromosome, and clines in inversion frequencies across southwestern United States indicate that strong natural selection operates them. Isogenic strains were made from isofemale lines collected four localities, eight molecular markers mapped chromosome. Nucleotide diversity was measured these loci formed basis of an evolutionary genomic analysis. The differentiated among inversions. did not show...
A French family had Charcot-Marie-Tooth disease type 2 (CMT2) which was characterised by late onset of peripheral neuropathy involvement, Argyll Robertson-like pupils, dysphagia, and deafness. Electrophysiological studies nerve biopsy defined the as axonal type. Genetic analysis myelin protein zero (MPZ) found a mutation in codon 124 resulting substitution threonine methionine. One patients, presently 30 years old, showed only pupils an objective sign but no clinical or electrophysiological...
Key to the remarkable ability of vestimentiferan tubeworms thrive in harsh conditions hydrothermal vents are hemoglobins that permit sequestration and delivery hydrogen sulfide oxygen chemoautotrophic bacteria. Here, we demonstrate zinc ions, not free cysteine residues, bind hemoglobins. The crystal structure C1 hemoglobin from vent tubeworm Riftia pachyptila has been determined 3.15 Å revealed unexpected presence 12 tightly bound Zn 2+ ions near threefold axes this D 3 symmetric hollow...
Abstract The alcohol dehydrogenase (Adh) region of Drosophila pseudoobscura, which includes the two genes Adh and Adh-Dup, was used to examine pattern organization linkage disequilibrium among pairs segregating nucleotide sites. A collection 99 strains from geographic range D. pseudoobscura were nucleotide-sequenced with polymerase chain reaction-mediated techniques. All 359 polymorphic sites in 3.5-kb tested for significant Fisher's exact test. Of 74,278 pairwise comparisons sites, 127 at...
ABSTRACT The alcohol dehydrogenase (Adh) locus (ADH; alcohol: NAD+ oxidoreductase, EC 1.1.1.1) of Drosophila pseudoobscura was cloned and sequenced. Forty-five percent the "effectively silent sites" have changed between Adh in D. obscura species group homologous DNA sequence mauritiana, latter representing melanogaster group. untranslated leader adult transcript has two deletions relative to mauritiana message. ADH protein sequences is missing third fourth amino acids at N-terminus enzyme....
The amylase region of the third chromosome Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series highly polymorphic inversions serves as convenient tool for molecular evolutionary analysis inverted gene arrangements. Amylase in D. family three genes, some chromosomes have deletions one or two them. Two overlapping clones covering 26 kilobases were isolated used probes survey DNA restriction map polymorphism among 28 lines,...
Abstract The genetic structure of Drosophila pseudoobscura populations was inferred from a nucleotide sequence analysis 3.4-kb segment the alcohol dehydrogenase (Adh) region. A total 99 isochromosomal strains collected 13 in North and South America were used to determine if any population departed neutral model estimate levels gene flow between populations. This study also included sequences two sibling species, D. persimilis miranda. We estimated mutation parameter, 4N mu, synonymous...
Abstract We have used the inversion system of Drosophila pseudoobscura to investigate how genetic flux occurs among gene arrangements. The patterns nucleotide polymorphism at seven loci were infer conversion events between pairs different estimate that average tract length is 205 bp and rate 3.4 × 10−6, which 2 orders magnitude greater than mutation rate. did not detect all combinations arrangements even though there was sufficient variation for detection opportunity exchanges occur. Genetic...
The population of Drosophila pseudoobscura at Bogota, Columbia, is geographically and partially reproductively isolated from populations in the main body species North America. degree genetic differentiation time divergence between Bogota Apple Hill, CA, were estimated by comparison 3388 nucleotides alcohol dehydrogenase region (Adh Adh-Dup genes) 18 strains. Of 146 polymorphic nucleotide sites detected, 68 31 unique to Hill samples, respectively, 53 shared. On basis an observed net per site...
Chromosomal rearrangements may play an important role in how populations adapt to a local environment. The gene arrangement polymorphism on the third chromosome of Drosophila pseudoobscura is model system help determine that inversions evolution this species. arrangements are likely target strong selection because they form classical clines across diverse geographic habitats, cycle frequency over seasons, and stable equilibria population cages. A numerical approach was developed estimate...