A5061685073- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Botanical Research and Chemistry
- Plant pathogens and resistance mechanisms
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Botany and Plant Ecology Studies
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Seed Germination and Physiology
- Plant Diversity and Evolution
- Plant tissue culture and regeneration
- DNA and Nucleic Acid Chemistry
- Glycosylation and Glycoproteins Research
- Allergic Rhinitis and Sensitization
- Microtubule and mitosis dynamics
- Enzyme Production and Characterization
- Plant Virus Research Studies
- Proteins in Food Systems
Mackay Memorial Hospital
2025
Pennsylvania State University
2011-2022
Intercollege
1996-2008
Washington State University
2003
Korea Research Institute of Bioscience and Biotechnology
1997
In-Q-Tel
1997
Cornell University
1984-1989
University of California, Los Angeles
1987
La Roche College
1983
Yale University
1983
Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300–400 copies of these sequences genome and approximately 100 human genome, Some realted shown to be thesepseudogenes indicating some pseudogenes transcrined. Rat cDNA 89% homologous coding region, 76% frist base paris 3'...
Microtubules influence morphogenesis by forming distinct geometrical arrays in the cell cortex, which turn affect deposition of cellulose microfibrils. Although many chemical and physical factors microtubule orientation, it is unclear how cortical microtubules elongating cells maintain their ordered transverse they reorganize into new geometries. To visualize these reorientations living cells, we constructed a reporter gene fusing binding domain mammalian microtubule-associated protein 4...
Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown encode the pollen determinant self-incompatibility. It postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), products of pistil determinant, inside tubes via ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it...
Sequences of 11 alleles the gametophytic self-incompatibility locus (S locus) from three species Solanaceae family have recently been determined. Pairwise comparisons these reveal two unexpected observations: (i) amino acid sequence similarity can be as low 40% within and (ii) some interspecific similarities are higher than intraspecific similarities. The gene genealogy clearly illustrates this unusual pattern relationships. data suggest that polymorphism at S existed prior to divergence has...
S proteins, pistil-specific ribonucleases that cosegregate with alleles, have previously been shown to control rejection of self-pollen in Petunia inflata and Nicotiana alata, two solanaceous species display gametophytic self-incompatibility. The ribonuclease activity proteins was thought degrade RNA tubes, resulting the arrest their growth style. However, date no direct evidence has obtained. Here, S3 protein P. abolished, effect on pistil's ability reject pollen examined. gene mutagenized...
Although the columella cells of root cap have been identified as site gravity perception, cellular events that mediate signaling remain poorly understood. To determine if cytoplasmic and/or wall pH mediates initial stages gravitropism, we combined a novel cell sensor (a cellulose binding domain peptide–Oregon green conjugate) and (plants expressing pH-sensitive fluorescent protein) to monitor dynamics throughout graviresponding Arabidopsis root. The apoplast acidified from 5.5 4.5 within 2...
Abstract Although pollen tube growth is essential for plant fertilization and reproductive success, the regulators of actin-related machinery cytosolic Ca2+ gradient thought to determine how these cells elongate remain poorly defined. Phospholipases, their substrates, phospholipid turnover products have been proposed as such regulators; however, relevant phospholipase(s) not characterized. Therefore, we cloned cDNA a pollen-expressed phosphatidylinositol 4,5-bisphosphate (PtdInsP2)–cleaving...
Abstract Petunia inflata S-locus F-box (Pi SLF) is thought to function as a typical protein in ubiquitin-mediated degradation and, along with Skp1, Cullin-1, and Rbx1, could compose an SCF complex mediating the of nonself S-RNase but not self S-RNase. We isolated three P. Skp1s SK1, -2, -3), two Cullin-1s CUL1-C -G), Rbx1 RBX1) cDNAs found that Pi CUL1-G did interact RBX1 none SKs interacted SLF2. also RING-HC protein, Binding Protein1 SBP1), almost identical hybrida SBP1, which interacts...
From a pollen tube cDNA library of Petunia inflata, we isolated clones encoding protein with structural features and biochemical properties characteristic receptor-like kinases. It was designated PRK1 for kinase 1. The cytoplasmic domain is highly similar to the domains other plant kinases contains nearly all conserved amino acids serine/threonine extracellular leucine-rich repeats as found in some kinases, but overall its sequence this region does not share significant similarity....
The function of the self-incompatibility locus (S locus) many plant species dictates that natural selection will favor high levels protein diversity. Pairwise sequence comparisons between S alleles from four Solanaceae reveal remarkably diversity and evidence for shared polymorphism. level amino acid constraint was found to be significantly heterogeneous among different regions gene, with some being highly constrained others appearing virtually unconstrained. In protein, there an excess...
We have isolated and sequenced the cytochrome oxidase subunit II gene from rice (OryzasativaL. var Labelle) . The overall structural organization of this is very similar to that maize gene. This contains an intron in a position identical However, longer than largely due 461 bp insertion sequence, which has inverted repeats at its termini flanked by direct repeats, characteristic transposable elements. Apart remainder sequence strikingly homologous (98.6X homology), suggesting possible...
Petunia possesses self-incompatibility, by which pistils reject self-pollen but accept non-self-pollen for fertilization. Self-/non-self-recognition between pollen and pistil is regulated the pistil-specific S-RNase gene multiple pollen-specific S-locus F-box (SLF) genes. To date, 10 SLF genes have been identified various methods, seven shown to be involved in specificity. For a given S-haplotype, each interacts with subset of its non-self S-RNases, an as yet unknown number SLFs are thought...