Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300–400 copies of these sequences genome and approximately 100 human genome, Some realted shown to be thesepseudogenes indicating some pseudogenes transcrined. Rat cDNA 89% homologous coding region, 76% frist base paris 3'...

Dinoflagellate taxonomy is based primarily on morphology and morphometric data that can be difficult to obtain. In contrast, molecular rapidly cost‐effectively acquired, which has led a rapid accumulation of sequence in GenBank. Currently there are no systematic criteria for utilizing taxonomically unassigned identify putative species could turn serve as basis testable hypotheses concerning the taxonomy, diversity, distribution, toxicity these organisms. The goal this research was evaluate...

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 63:33-41 (2005) - doi:10.3354/dao063033 Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes Carolyn S. Friedman1,*, Robyn M. Estes1, Nancy A. Stokes2, Colleen Burge1, John Hargove1, Bruce J. Barber3,5, Ralph Elston4, Eugene Burreson2,...

A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass ( Morone saxatilis ) during an epizootic mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those the genus Mycobacterium . Biochemical reactions, growth characteristics mycolic acid profiles (HPLC) resembled shottsii , a non-pigmented mycobacterium also same epizootic. Sequencing genes, encoding exported repeated protein erp 65...

Eighteen microsatellite markers were developed for the Crassostrea virginica nuclear genome, including di-, tri-, and tetranucleotide repeat regions that included perfect, imperfect, compound sequences. A reference panel with DNA from parents four progeny of 10 full-sib families was used a preliminary confirmation polymorphism at these loci indications null alleles. Null alleles discovered three loci; in two instances, primer redesign enabled their amplification. Two to five representative...

The putative harmful algal bloom dinoflagellate, Pfiesteria piscicida (Steidinger et Burkholder), frequently co‐occurs with other morphologically similar species collectively known as ‐like organisms (PLOs). This study specifically evaluated whether unique sequences in the internal transcribed spacer (ITS) regions, ITS1 and ITS2, could be used to develop PCR assays capable of detecting PLOs natural assemblages. ITS regions were selected because they are more variable than flanking small...

ABSTRACT The protistan parasite Perkinsus marinus is a severe pathogen of the oyster Crassostrea virginica along east coast United States. Very few data have been collected, however, on abundance in environmental waters, limiting our understanding P. transmission dynamics. Real-time PCR assays with SybrGreen I as label for detection were developed this study quantification waters species-specific primers and spp. genus-specific primers. Detection DNA concentrations low equivalent 3.3 × 10 −2...

Harmful algal bloom (HAB) species in the Chesapeake Bay can negatively impact fish, shellfish, and human health via production of toxins degradation water quality. Due to deleterious effects HAB on economically environmentally important resources, such as oyster reef systems, area resource managers are seeking ways monitor HABs quality at large spatial fine temporal scales. The use satellite ocean color imagery has proven be a beneficial tool for management other locations around world where...

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 50:51-65 (2002) - doi:10.3354/dao050051 Study perkinsosis in carpet shell clam Tapes decussatus Galicia (NW Spain). I. Identification aetiological agent and vitro modulation zoosporulation by temperature salinity Sandra M. Casas1, Antonio Villalba1,*, Kimberly S. Reece2 1Centro de Investigacións Mariñas, Consellería...

An international community of biologists presents the Pacific oyster Crassostrea gigas as a candidate for genome sequencing. This has global distribution and past several years highest annual production any freshwater or marine organism (4.2 million metric tons, worth $3.5 billion US). Economic cultural importance oysters motivates great deal biologic research, which provides compelling rationale sequencing an genome. Strong rationales also come from contrasts to other genomes: membership in...

The phylogenetic affinities of the oyster pathogen Perkinsus marinus were investigated with morphology, 18S-like rDNA data and actin sequence data. Morphological investigations revealed that species do not have a conoid other criteria which been used to place them in Apicomplexa are general alveolates. When considered separately, sets each support closer affinity for dinoflagellates. However, these separate analyses possess their own biases weaknesses. Use principle ‘total evidence’ combined...

ABSTRACT. Perkinsus species are destructive parasites of commercial Manila clams, Venerupis philippinarum , in Japan, Korea, and Spain. However, vitro parasite cultures from this important host clam not available. Tissues clams collected during April 2002 Gokasho Bay, Japan harbored sp. at a 97% prevalence (28/29) moderate‐ high‐intensity infections. cells tissue samples were enlarged alternative Ray's fluid thioglycollate medium, before propagation DME:Ham's F‐12 culture medium. Enlarged...

ABSTRACT. Oysters were collected from coastal locations in China 1999–2006 for parasite analyses by molecular, culture, and histological techniques. Polymerase chain reaction‐based assays targeting the internal transcribed spacer (ITS) region of ribosomal RNA gene complex performed to detect presence Perkinsus species. Sequencing phylogenetic analysis amplified sp. DNAs indicated that a novel infects Crassostrea hongkongensis, ariakensis , other bivalve hosts Fujian Guangxi provinces...

<title>Abstract</title> As a state with high shellfish aquaculture production in the US, it is essential that New Jersey (NJ) managers identify phycotoxins of concern and their spatiotemporal distribution. However, to date, there are no publications on phycotoxin presence or concentrations NJ estuaries. Passive samplers valuable for assessing emerging threats long-term trends phycotoxins, but levels phenolic compounds from Pinelands may interfere by competing binding sites resin. The...

Perkinsus species presently are classified within the phylum Apicomplexa. This placement, however, is controversial. Based upon morphological observations and phylogenetic analyses of small subunit ribosomal RNA gene, it has been suggested that may be more closely related to dinoflagellates. To reevaluate position Perkinsus, we obtained nucleotide sequence data for actin genes from marinus 2 dinoflagellates, Prorocentrum minimum Amphidinium carterae. Results indicated there in genome P....

Phylogenetic analysis conducted on a 784-bp fragment of 82 actin gene sequences 44 coleoid cephalopod taxa, along with results obtained from genomic Southern blot analysis, confirmed the presence at least three distinct loci in coleoids. Actin isoforms were characteri zed through phylogenetic representative each isoforms, translated cDNA diverse array metazoan taxa downloaded GenBank. One found cephalopods was closely related to expressed muscular tissues other molluscs. A second isoform...

Abstract. Diverse analytical and experimental results confirm that two protistan parasites, Perkinsus chesapeaki andrewsi , described separately as parasites of Mya arenaria Macoma balthica clams sympatric in Chesapeake Bay, USA, represent a single species. Ribosomal RNA (rRNA) internal transcribed spacer (ITS) regions, rRNA large subunit (LSU) gene, actin gene sequences were obtained from clonal spp. cultured vitro. Although multiple polymorphic found DNA cultures at each locus, identical...

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 77:207-223 (2007) - DOI: https://doi.org/10.3354/dao01829 Pathogens in Crassostrea ariakensis and other Asian oyster species: implications for non-native introduction Chesapeake Bay J. A. Moss1, E. M. Burreson1, F. Cordes1, C. Dungan2, G. D. Brown1, Wang3, X. Wu4, K. S. Reece1,* 1Virginia Institute Marine Science,...

Perkinsus marinus and P. chesapeaki host ranges among wild Chesapeake Bay, USA, region bivalves were examined by surveying Crassostrea virginica oysters members of several sympatric clam species from 11 locations. genus- species-specific PCR assays performed on DNA samples 731 molluscs, in situ hybridization a selected subset histological whose results indicated dual or atypical sp. infections. detected 92% oysters, but the assay was positive for only 6% one clam. The very low prevalence...