- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Marine Biology and Ecology Research
- Water Quality Monitoring and Analysis
- Crustacean biology and ecology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Community Ecology and Physiology
- Oceanographic and Atmospheric Processes
- Geochemistry and Geologic Mapping
- Protist diversity and phylogeny
- Cell Image Analysis Techniques
- Oil Spill Detection and Mitigation
- Ocean Acidification Effects and Responses
- Marine Invertebrate Physiology and Ecology
- Isotope Analysis in Ecology
- Marine and fisheries research
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Advanced Chemical Sensor Technologies
- Bioactive natural compounds
- Coastal and Marine Management
- Coral and Marine Ecosystems Studies
Mote Marine Laboratory
2017-2024
William & Mary
2002-2003
Florida red tides are harmful algae blooms caused by the dinoflagellate Karenia brevis, which occur along Florida's gulf coast almost annually. In recent years tide have become more common, frequent, and intense. southwest coast, from Manatee to Collier County, has experienced repeated prolonged K. brevis since 2011 with most bloom in 2017 lasting 17 months resulting both hypoxic anoxic events. We therefore determined survival level of lethargy (e.g., lack responsiveness or reduction...
In Fall 2017 a large bloom of the toxic dinoflagellate Karenia brevis developed in Gulf Mexico. After persisting for months, 2018 wind and water circulation patterns drove K. towards east coast Florida. On September 29, Palm Beach County, FL beaches were closed due to respiratory gastrointestinal issues associated with brevotoxins, effects brevotoxins reported from within estuarine segments Loxahatchee River Estuary (LRE). This was first apparent report impacting inshore portions LRE...
We apply a neural network (NN) technique to detect/track Karenia brevis harmful algal blooms (KB HABs) plaguing West Florida shelf (WFS) coasts from Visible-Infrared Imaging Radiometer Suite (VIIRS) satellite observations. Previously KB HABs detection primarily relied on the Moderate Resolution Spectroradiometer Aqua (MODIS-A) satellite, depending its remote sensing reflectance signal at 678-nm chlorophyll fluorescence band (Rrs678) needed for normalized height and related red difference...
Water quality, microbial contamination, prior fish health, and variable results have been major impediments to identifying the cause mechanism of mortality in standard aquarium‐format Pfiesteria bioassays. Therefore, we developed a sensitive 96‐h larval bioassay for assessing spp. pathogenicity using six‐well tissue culture plates 7‐day‐old cyprinodontid fish. We used assay test several clonal lines piscicida Steidinger Burkholder P. shumwayae Glasgow that had cultured with algal prey 2 36...
Curcumin, a natural plant product, was investigated as mitigation tool against Karenia brevis, the toxic dinoflagellate responsible for Florida red tides. A series of laboratory bench-top studies were conducted with additions 0.1, 1, 2, 3, 5, 10, 20, 30, and 40 mg/L curcumin to K. brevis at an average 1.0 × 106 cells/L determine efficacy cells brevetoxins optimize treatment dosage. Treatment 5 reduced cell abundance by 89% total 60% within 24 h. Lower concentrations (0.1–3 mg/L) exhibited...
Preliminary results of previous work had shown a Neural Network (NN) technique developed by us as effective in detecting Karenia brevis Harmful Algal Blooms (KB HABs) plaguing West Florida Shelf (WFS) from VIIRS satellite observations. We extend comparisons NN retrievals against data set near simultaneous in-situ measurements the WFS spanning 2012-2016 period for which there was available data. Specifically we looked match ups where overlap time windows between observations and were 15...
We examine the potential for ocean color (OC) retrievals using a neural network (NN) technique recently developed by us to make up lack of 678 nm florescence band on VIIRS, previously available MODIS and important Karenia brevis harmful algal bloom (KB HABs) retrievals.. NN uses VIIRS Remote Sensing Reflectance (Rrs) at 486, 551 671 retrieve phytoplankton absorption 443nm, from which both KB HABs chlorophyll [Chla] concentrations can be inferred. are compared with obtained other algorithms,...
We examine the impact of temporal changes on satellite retrievals Karenia brevis Harmful Algal blooms (KB HABS) in West Florida Shelf (WFS). These impacts are compared for from both VIIRS and MODIS-A using a number retrieval techniques. The comparisons include our recently developed neural network (NN) technique. network, previously by us, was trained 10,000 data point part synthetic 20,000 inherent optical properties (IOPs) based wide range IOP parameters large variety natural conditions...
Karenia brevis Harmful Algal blooms (KB HABS) plague the coasts of West Florida Shelf (WFS) and effective monitoring is needed to provide information local authorities for health warnings etc. We compare results satellite retrievals KB HABs, using previously existing algorithms, both MODIS VIIRS, as well with our more recently developed neural network (NN) algorithm. Retrievals are compared against in-situ HABs measurements. To obtain sufficient numbers measurements nearly concurrent...
We examine the potential for ocean color (OC) retrievals using a neural network (NN) technique recently developed by us to make up lack of 678 nm fluorescence band on VIIRS, previously available MODIS and important Karenia brevis harmful algal bloom (KB HABs) retrievals. NN uses VIIRS Remote Sensing Reflectance (Rrs) at 486, 551 671 retrieve phytoplankton absorption 443nm, from which both chlorophyll [Chla] concentrations KB HABs can be inferred. are compared with obtained different...
Our previous work showed the efficacy neural network (NN) approaches for satellite detection of Karenia brevis (KB) harmful algal blooms (HABs) in West Florida Shelf (WFS). Applying a multiband NN, trained on wide range synthetically simulated inherent optical properties (IOPs), NN takes Visible Infrared Imaging Radiometer Suite (VIIRS) remote sensing reflectance (Rrs) measurements at 486, 551 and 671 nm bands as inputs produces related IOPs 443 nm, including absorption coefficient...
Brevetoxins (PbTx) are a group of marine algal toxic compounds that produced by the bloom-forming dinoflagellate Karenia brevis . During harmful blooms (HAB), brevetoxins can reach concentrations high enough to cause human neurological and gastrointestinal illnesses through inhalation airborne toxins or consumption contaminated shellfish. The timely recognition this toxin in environment is great importance for public safety, health, recreation, ecology, economy. We present development...