Four derivatives of thymol, carvacrol, and eugenol were synthesized: 4-(hydroxymethyl)-5-isopropyl-2-methylphenol, 4,4′-methylenebis(5-isopropyl-2-methyl)phenol, 4-allyl-6-(hydroxymethyl)-2-methoxyphenol, 4-(hydroxymethyl)-2-isopropyl-5-methylphenol. The obtained showed remarkably better antioxidative properties according to 1,1-diphenyl-2-picrylhydrazyl assay (50% inhibitory concentrations = 4–156 µg/mL) Rancimat (protection factors 1.55–5.84) when compared with parent compounds values...

Finding a new type of cholinesterase inhibitor that would overcome the brain availability and pharmacokinetic parameters or hepatotoxic liability has been focus investigations dealing with treatment Alzheimer's disease. Isothiocyanates have not previously investigated as potential inhibitors. These compounds can be naturally produced from their glucosinolate precursors, secondary metabolites widely distributed in our daily Brassica vegetables. Among 11 tested compounds, phenyl isothiocyanate...

The purpose of this study was to compare the essential oil profiles four Croatian Teucrium species (Lamiaceae), as determined by GC and GC/MS, with their antiphytoviral efficiency. A phytochemical analysis showed that T. polium, flavum, montanum chamaedrys are characterized similar compositions. investigated oils a high proportion sesquiterpene hydrocarbons β-caryophyllene (7.1–52.0%) germacrene D (8.7–17.0%). Other important components were β-pinene from α-pinene flavum. proved reduce...

Nasturtium officinale R. Br. (watercress) is an endangered species with valuable pharmaceutical, cosmetic, and nutritional properties. The purpose of this work was to evaluate the phytochemical profile biological activity extracts from microshoot cultures grown in PlantForm bioreactors parent plant material. After 20 days cultivation, achieved best results both terms key active ingredient content activity. glucosinolates (GSL) by UHPLC-DAD-MS/MS method showed that dominant compounds were...

The glucosinolate (GSL) profiles in different plant parts of the wild-growing species Lepidium draba L. (syn. Cardaria Desv.) (Brassicaceae) from Tunisia and Croatia were determined using UHPLC-DAD-MS/MS. predominant GSLs both Tunisian Croatian samples glucoraphanin glucosinalbin, with leaves flowers being richest sources. study also revealed previously unreported draba, methionine-derived glucoiberin, glucoalyssin, glucohesperin, glucolesquerellin, as well tryptophan-derived glucobrassicin...

ABSTRACT In this study, essential oils from wild-grown caper (Capparis spinosa L.) and sea fennel (Crithmum maritimum Dalmatia (Croatia) were isolated by hydrodistillation analyzed gas chromatography with mass spectrometry. Methyl isothiocyanate (92.06%) was detected as major component of oil leaves flower buds, while limonene (58.37%), sabinene (26.46%), terpinene-4-ol (5.59%) γ-terpinene (2.81%) identified dominant compounds in oil. Tested did not exhibit radical-scavenging activity...

Abstract Two different volatile isolates from the aerial parts of Cardaria draba (L.) Desv., obtained either by hydrodistillation ( Extract I ) or CH 2 Cl extraction subsequent to hydrolysis exogenous myrosinase II ), were characterized GC‐FID and GC/MS analyses. The main volatiles hydrodistillation, i.e. , 4‐(methylsulfanyl)butyl isothiocyanate 1 ; 28.0%) 5‐(methylsulfanyl)pentanenitrile 13.8%), originated degradation glucoerucin. In also without sulfur and/or nitrogen identified. These...

Abstract The cultivated Lepidium latifolium L. was investigated to decipher its glucosinolate profile, antimicrobial, and cytotoxic activities. HPLC/ESI‐MS analyses of the intact glucosinolates GC/MS analysis their hydrolysis products showed presence sinigrin ( 1 ), glucocochlearin 2 glucotropaeolin 3 4‐methoxyglucobrassicin 4 ). Hydrodistillate, extract, allyl isothiocyanate, main volatile resulting from degradation, antimicrobial activity against all eleven tested pathogenic food spoilage...

Horseradish degradation products, mainly isothiocyanates (ITC) and nitriles, along with their precursors glucosinolates, were characterized by GC-MS UHPLC-MS/MS, respectively. Volatiles from horseradish leaves roots isolated using microwave assisted-distillation (MAD), hydrodiffusion gravity (MHG) hydrodistillation (HD). Allyl ITC was predominant in the regardless of isolation method while MAD, MHG, HD resulted different yields allyl ITC, 2-phenylethyl nitriles. The antimicrobial potential...

Abstract The study has proved the stimulating effects of different strategies treatments with elicitors on production glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in watercress ( Nasturtium officinale ) microshoot cultures. also assessed antioxidant anti-melanin activities. following were tested: ethephon (ETH), methyl jasmonate (MeJA), sodium salicylate (NaSA), yeast extract (YeE) added day 10 growth period. Cultures not treated elicitor used as...

Glucosinolates (GSLs) are secondary metabolites mainly found in the plant order Brassicales. We critically evaluate reports of GSLs other orders flowering plants, propose standards for future and subject two cases to rigorous testing. Reports non-Brassicales species should live up state-of-the-art scientific concerning chemical evidence, botanical evidence biological replication. Occurrence family Putranjivaceae, Malpighiales (genera Putranjiva Drypetes) was reasonably supported, but...

Abstract Volatile fractions of rocket ( Eruca sativa Mill.) flowers and leaves with green beans were isolated by hydrodistillation in a Clevenger apparatus from fresh plant material prior to after autolysis dried material. All isolates volatile compounds analysed gas chromatography/gas chromatography–mass spectrometry (GC/GC–MS). Sulphur and/or nitrogen compounds, fatty acids esters, aliphatic alcohols carbonyl some other smaller percentages identified. The main sulphur components...

Abstract Volatile compounds of hedge mustard ( Sysimbrium officinale ) have been investigated for the first time. Forthy‐two were identified after hydrodistillation (without or upon autolysis) gas chromatography and chromatography/mass spectrometry analyses. In addition, decoction hydrolysis O ‐glycosides, 18 volatile ‐aglycones identified. general, main volatiles found in hydrodistillates were: isopropyl isothiocyanate (27.6–48.9%), 2‐methylpropanenitrile (0.5–18.8%), Z )‐hex‐3‐en‐1‐ol...

Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of Euro-Mediterranean area. The aim present study was determine glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, cytotoxic activities) isolates containing breakdown products. chemical profiles were determined by using HPLC-PDA-MS/MS desulfoglucosinolates GC-MS degradation analysis B. showed presence seven glucosinolates: gluconapin (1),...

Abstract Glucosinolates (GLs) were characterized in various aerial parts (stems, leaves, and flowers) of Aurinia leucadea ( Guss .) C. Koch quantified according to the ISO 9167‐1 official method based on HPLC analysis desulfoglucosinolates. Eight GLs, i.e. , glucoraphanin (GRA), glucoalyssin (GAL; 1 ), gluconapin (GNA; 2 glucocochlearin (GCC), glucobrassicanapin (GBN; 3 glucotropaeolin (GTL), glucoerucin (GER), glucoberteroin (GBE) identified. The total GL contents 57.1, 37.8, 81.3 μmol/g...

The main compounds in both extracts were gluconasturtiin, 4-methoxyglucobrassicin and rutoside, the amounts of which were, respectively, determined as 182.93, 58.86 23.24 mg/100 g dry weight (DW) biomass 640.94, 23.47 7.20 DW plant herb extracts. antioxidant potential all studied evaluated using CUPRAC (CUPric Reducing Antioxidant Activity), FRAP (Ferric Ability Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl) assays was comparable. anti-inflammatory activity tested based on inhibition...

Glucosinolates (GSLs) from Lunaria annua L. seeds were analyzed qualitatively and quantitatively by their desulfo counterparts using UHPLC-DAD-MS/MS technique volatile breakdown products, isothiocyanates (ITCs), GC-MS technique. GSL products obtained conventional techniques (hydrodistillation in a Clevenger type apparatus (HD), CH2Cl2 extraction after myrosinase hydrolysis (EXT) for 24 h) as well modern techniques, microwave-assisted distillation (MAD) microwave hydrodiffusion gravity (MHG)....

Abstract Free and glucosidic bound leaf volatiles of Degenia velebitica were isolated fractionated simultaneously into H 2 O‐soluble, O‐insoluble, highly volatile compounds by hydrodistillation–adsorption (HDA) analyzed GC/MS. Among the 24 constituents identified, main obtained HDA method S‐ and/or N‐atom containing compounds, i.e. , 6‐(methylsulfanyl)hexanenitrile ( 10 ; 26.78%), dimethyl trisulfide 6 26.35%), 3,4,5‐trimethylpyrazole 17 13.33%), hex‐5‐enenitrile 10.11%), tetrasulfide 8...