A5103074430- Gut microbiota and health
- Probiotics and Fermented Foods
- Reproductive tract infections research
- Corrosion Behavior and Inhibition
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Pelvic floor disorders treatments
- Amphibian and Reptile Biology
- Hydrogen embrittlement and corrosion behaviors in metals
- Enterobacteriaceae and Cronobacter Research
- Advanced Chemical Sensor Technologies
- Concrete Corrosion and Durability
- Plant-Microbe Interactions and Immunity
- Sensory Analysis and Statistical Methods
- Recycling and Waste Management Techniques
- CO2 Sequestration and Geologic Interactions
- Adolescent Sexual and Reproductive Health
- Non-Destructive Testing Techniques
- Bacteriophages and microbial interactions
- Pharmacological Effects of Natural Compounds
- Material Selection and Properties
- Plant Disease Resistance and Genetics
- RNA modifications and cancer
- Clostridium difficile and Clostridium perfringens research
- Concrete and Cement Materials Research
Centers for Disease Control and Prevention
2016-2022
National Center for Environmental Health
2017-2022
The Centers
2016-2021
North Carolina State University
2021
Oak Ridge Associated Universities
2016
University of Illinois Urbana-Champaign
2009-2013
Utica College
2009-2011
Urbana University
2010
Lawrence Livermore National Laboratory
1997-2004
The bacterial communities present in smokeless tobacco (ST) products have not previously reported. In this study, we used Next Generation Sequencing to study the bacteria U.S.-made dry snuff, moist snuff and Sudanese toombak. Sample diversity taxonomic abundances were investigated these products. A total of 33 families from four phyla, Actinobacteria, Firmicutes, Proteobacteria Bacteroidetes, identified. U.S.-produced contained a diverse distribution all phyla. Moist dominated by Firmicutes....
Abstract Humans and baboons ( Papio spp.) share considerable anatomical physiological similarities in their reproductive tracts. Given the similarities, it is reasonable to expect that normal vaginal microbial composition (microbiota) of would be similar humans. We have used a 16S rRNA phylogenetic approach assess baboon microbiota set nine animals from captive facility six wild. Results show although Gram‐positive bacteria dominate as they do humans, there are major differences between In...
Abstract The bacterial population of the primate vaginal canal is an infant primate's first exposure to microbial inhabiting outside world. Yet, little known about this and effect it might have on development survival primate. As a step toward characterizing microbiota nonhuman primate, we used denaturing gradient gel electrophoresis evaluate variations in group 35 baboons ( Papio hamadryas ), which were housed facility where they shared same diet environmental conditions. We found that,...
Previous studies of resistance gene ecology have focused primarily on populations such as hospital patients and farm animals that are regularly exposed to antibiotics. Also, these tended focus numerically minor enterics or enterococci. We report here a cultivation-independent approach allowed us assess the presence antibiotic genes in predominant vaginal microbiota two primates seldom never antibiotics: baboons mangabeys. Most were part captive colony Texas is used for scientific female...
Abstract Smokeless tobacco (ST) products are used worldwide and a major public health concern. In addition to harmful chemicals found in these products, microbes ST believed be responsible for generating tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens ST. These also contribute endotoxins other pro-inflammatory components. A greater understanding of microbial constituents is sought order potentially link select design aspects or manufacturing processes avoidable increases...
Appreciation of the importance microbiome is increasing, as sequencing technology has made it possible to ascertain microbial content a variety samples. Studies that sequence 16S rRNA gene, ubiquitous in and nearly exclusive bacteria, have proliferated medical literature. After sequences are binned into operational taxonomic units (OTUs) or species, data from these studies summarized matrix with observed counts each OTU for sample. Analysis often reduces further pairwise distances...
Microbiological growth parameters, including limiting factors, kinetics, and minimal cell densities were assessed for subsurface microbiological communities collected with rock from an area proposed a nuclear waste repository at Yucca Mountain, Nevada. Phospholipid fatty acid analysis revealed that approximately 104–105 viable cells per gram of dry are extant, water availability was shown to be the primary factor microbial in situ. Phosphate carbon limitation, however, also suppress final by...
Smokeless tobacco (ST) products are widely used throughout the world and contribute to morbidity mortality in users through an increased risk of cancers oral diseases. Bacterial populations ST taste, but their presence can also create carcinogenic, Tobacco-Specific N-nitrosamines (TSNAs). Previous studies microbial communities lacked chemistry data (e.g. nicotine, TSNAs) characterize identify associations between carcinogen levels taxonomic groups. This study uses statistical analysis...
The most alarming aspect of the Sudanese toombak smokeless tobacco is that it contains high levels highly toxic tobacco-specific nitrosamines (TSNAs). Understanding microbiology relevance because TSNAs are an indirect result microbial-mediated nitrate reductions. We conducted shotgun metagenomic sequencing on a product for which relevant features presented here. microbiota was composed over 99% Bacteria. abundant taxa included Actinobacteria, specifically genera Enteractinococcus and...
It is known that data from both 16S and shotgun metagenomics studies are subject to biases cause the observed relative abundances of taxa differ their true values. Model community analyses, in which all sample by construction, seem offer hope these can be measured. However, it unclear whether bias we measure a mock analysis same as spiked at abundance, or if spike-in samples would real (e.g., biological) sample. Here, consider questions context rRNA measurements on three sets samples:...
Abstract An intact gut microbiota confers colonization resistance against Clostridioides difficile through a variety of mechanisms, likely including competition for nutrients. Recently, proline was identified as an important environmental amino acid that C. uses to support growth and cause significant disease. A post-translationally modified form, trans-4-hydroxyproline, is highly abundant in collagen, which degraded by host proteases response toxin activity. The ability dehydrate...