A5102778603- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- RNA Research and Splicing
- Planarian Biology and Electrostimulation
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Biomimetic flight and propulsion mechanisms
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Fish biology, ecology, and behavior
- Genetics, Aging, and Longevity in Model Organisms
- Axon Guidance and Neuronal Signaling
- Cytokine Signaling Pathways and Interactions
- Renal and related cancers
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Genomics and Phylogenetic Studies
- Cellular Mechanics and Interactions
- Kruppel-like factors research
- RNA modifications and cancer
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
University of Chicago
2010-2021
Minneapolis Heart Institute Foundation
2018
University of Minnesota
2018
Princeton University
1997-2005
University of Oregon
1990-2001
Howard Hughes Medical Institute
2001
Ludwig Cancer Research
2001
The Royal Melbourne Hospital
2001
University of California, Berkeley
1986-2000
Center for Human Genetics
1998
HOX genes specify cell fate in the anterior-posterior axis of animal embryos. Invertebrate chordates have one cluster, but mammals four, suggesting that cluster duplication facilitated evolution vertebrate body plans. This report shows zebrafish seven hox clusters. Phylogenetic analysis and genetic mapping suggest a chromosome doubling event, probably by whole genome duplication, after divergence ray-finned lobe-finned fishes before teleost radiation. Thus, teleosts, most species-rich group...
ABSTRACT Embryos mutant for the T gene, in mice, make insufficient mesoderm and fail to develop a notochord. We report cloning sequencing of gene zebrafish (Brachydanio rerio) show nuclear localization protein product. Both RNA are found cells germ ring, including enveloping layer cells, prior during gastrulation embryos. Nuclei yolk syncytial do not express Zf-T. High levels expression maintained throughout early development notochord, while paraxial is turned off gastrulation. Exposure...
The Actinopterygii (ray-finned fishes) is the largest and most diverse vertebrate group, but little agreed about timing of its early evolution. Estimates using mitochondrial genomic data suggest that major actinopterygian clades are much older than divergence dates implied by fossils. Here, evolutionary origins these reinvestigated morphological, nuclear genetic data. Results indicate existing fossil-based estimates age crown-group Neopterygii, including teleosts, Lepisosteus (gar) Amia...
The precursors of several organs reside within the lateral plate mesoderm vertebrate embryos. Here, we demonstrate that zebrafish hands off locus is essential for development two structures derived from - heart and pectoral fin. mutant embryos have defects in myocardial an early stage: they produce a reduced number precursors, tissue does form improperly patterned fails to maintain tbx5 expression. A similar array observed differentiation fin mesenchyme: small buds delayed fashion,...
We have examined the expression of a Hairy/E(spl)-related (Her) gene, her7, in zebrafish and show that its PSM cycles similarly to her1 deltaC. A decrease her7 function generated by antisense oligonucleotides disrupts somite formation posterior trunk tail, dynamic domains deltaC, suggesting plays role coordinating oscillations neighboring cells presomitic mesoderm. This phenotype is reminiscent segmentation mutants with lesions genes Delta/Notch signaling pathway, which also disruption...
The developing hindbrain is organized into a series of segments termed rhombomeres which represent lineage restricted compartments correlating with domains gene expression and neuronal differentiation. In this study, we investigate the processes segmentation acquisition segmental identity by analyzing zebrafish hox genes in hindbrains normal fish loss-of-function mutation valentino (val, homologue mouse kreisler; Moens, C. B., Cordes, S. P. Giorgianni, M. W., Barsh, G. Kimmel, B. (1998)....
ABSTRACT The Hox genes are implicated in conferring regional identity to the anteroposterior axis of developing embryo. We have characterized organization and expression hox teleost zebrafish (Danio rerio), compared our findings with those made for tetrapod vertebrates. isolated 32 genes, primarily via 3′RACE-PCR, analyzed their linkage relationships using somatic cell hybrids. find that comparison tetrapods, has several additional both within beyond expected 4 clusters (A-D). For example,...
The van gogh (vgo) mutant in zebrafish is characterized by defects the ear, pharyngeal arches and associated structures such as thymus. We show that vgo caused a mutation tbx1, member of large family T-box genes. tbx1 has been recently suggested to be major contributor cardiovascular DiGeorge deletion syndrome (DGS) humans, which several neural crest derivatives are affected arches. Using cell transplantation studies, we demonstrate vgo/tbx1 acts autonomously mesendoderm influences...
ABSTRACT In order to understand the developmental mechanisms of posterior body formation in zebrafish, a fate map zebrafish tailbud was generated along with detailed analysis cell movements. The shows that it contains tissue-restricted domains and is not homogeneous blastema. Furthermore, time-lapse some movements behaviors are similar those seen during gastrulation, while others unique body. extension axial mesoderm continuation ingression throughout tail development suggests processes...
Zebrafish floating head mutant embryos lack notochord and develop somitic muscle in its place. This may result from incorrect specification of the domain at gastrulation, or respecification progenitors to form muscle. In genetic mosaics, acts cell autonomously. Transplanted wild-type cells differentiate into hosts; however, donors produce rather than hosts. Consistent with respecification, markers axial mesoderm are initially expressed gastrulas, but expression does not persist. Axial also...
When do single cells in the early zebrafish embryo become irreversibly committed to a specific fate? Work with lineage tracing and fate mapping has shown that marginal of blastoderm give rise hypoblast-derived fates (mesoderm endoderm). However, experiments described here show these remain pluripotent uncommitted throughout late blastula gastrula stages. Embryonic at mid-gastrulation. Time-lapse photographic analysis reveals cells, when transplanted heterotopically heterochronically, can...
Determination of fate maps and cell lineage tracing have previously been carried out in the zebrafish embryo by following progeny individual cells injected with fluorescent dyes. We review information obtained from these experiments then present an approach to mapping movement tracing, utilizing activation caged fluorescein-dextran. This method has several advantages over single-cell injections that it is rapid, allows at all depths be marked, can used follow starting any time during...
Codevelopment of the lungs and heart underlies key evolutionary innovations in transition to terrestrial life. Cardiac specializations that support pulmonary circulation, including atrial septum, are generated by second field (SHF) cardiopulmonary progenitors (CPPs). It has been presumed transcription factors required SHF for cardiac septation, e.g., Tbx5 , directly drive a morphogenesis gene-regulatory network. Here, we report instead TBX5 drives Wnt ligands initiate bidirectional signaling...