A5038806506- Ion Channels and Receptors
- Cancer, Stress, Anesthesia, and Immune Response
- Menstrual Health and Disorders
- Advanced Fluorescence Microscopy Techniques
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- RNA modifications and cancer
- Genomics, phytochemicals, and oxidative stress
- Cancer-related molecular mechanisms research
- Clusterin in disease pathology
- Tryptophan and brain disorders
- Protein Structure and Dynamics
- CCD and CMOS Imaging Sensors
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Pharmacological Effects and Toxicity Studies
- Neuropeptides and Animal Physiology
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
Charles University
2021-2024
Abstract Background The transient receptor potential vanilloid 1 (TRPV1) is well-established in neuronal function, yet its role immune reactions remains enigmatic. conflicting data on inflammatory role, suggesting both pro-inflammatory and anti-inflammatory effects upon TRPV1 stimulation cells, adds complexity. To unravel immunomodulatory mechanisms, we investigated how the agonist capsaicin influences lipopolysaccharide (LPS)-induced macrophage phenotypes. Results Changes surface molecules,...
The pathogenesis of Alzheimer's disease (AD), the most prevalent form dementia, remains unclear. Over past few years, evidence has accumulated indicating that perturbed cerebral bioenergetics and neuroinflammation may compromise cognitive functions precedes onset AD impaired function glial cells can likely contribute to development disease. Recently, N6-methyladenosine (m6A) modification RNA been implicated in regulation different processes brain play a potential role neurodegeneration. In...
Opioids are known to have antioxidant effects and modulate microglial function under certain conditions. It has been previously shown that opioid ligands can effectively inhibit the release of proinflammatory cytokines when stimulated with lipopolysaccharide (LPS) convert microglia an anti-inflammatory polarization state. Here, we used C8-B4 cells, mouse cell line activated by LPS as a model investigate anti-inflammatory/antioxidant potential selected receptor agonists (DAMGO, DADLE,...
De novo genes emerge from previously noncoding stretches of the genome. Their encoded de proteins are generally expected to be similar random sequences and, accordingly, with no stable tertiary fold and high predicted disorder. However, structural properties whether they differ during stages emergence fixation have not been studied in depth rely heavily on predictions. Here we generated a library short human putative varying lengths ages sorted candidates according their compactness disorder...
Abstract De novo genes emerge from previously non-coding stretches of the genome. Their en-coded de proteins are generally expected to be similar random sequences and, accordingly, with no stable tertiary fold and high predicted disorder. However, structural properties whether they differ during stages emergence fixation have not been studied in depth rely heavily on predictions. Here we generated a library short human putative varying lengths ages sorted candidates according their...
Methadone is commonly used as an alternative to morphine in patients with pain associated glioblastoma and other cancers. Although concomitant administration of methadone cytostatics relatively common, the effect on efficacy cytostatic drugs has not been well studied until recently. Moreover, mechanism behind temozolomide investigated previous studies, or this automatically attributed opioid receptors. Our findings indicate that potentiates rat C6 cells human U251 T98G increases cell...
Abstract The glycoprotein clusterin (CLU) is involved in cell proliferation and DNA damage repair highly expressed tumor cells. Here, we aimed to investigate the effects of CLU dysregulation on two human astrocytic lines: CCF-STTG1 astrocytoma cells SV-40 immortalized normal astrocytes. We observed that suppression expression by RNA interference inhibited proliferation, triggered response, resulted cellular senescence both types tested. To further underlying mechanism behind these changes,...
<title>Abstract</title> Background: The transient receptor potential vanilloid 1 (TRPV1) is well-established in neuronal function, yet its role immune reactions remains enigmatic. conflicting data on inflammatory role, suggesting both pro-inflammatory and anti-inflammatory effects upon TRPV1 stimulation cells, adds complexity. To unravel immunomodulatory mechanisms, we investigated how the agonist capsaicin influences lipopolysaccharide (LPS)-induced macrophage phenotypes. Results: Changes...