A5059479931- Osteoarthritis Treatment and Mechanisms
- Electrostatics and Colloid Interactions
- Knee injuries and reconstruction techniques
- Advanced Materials and Mechanics
- Polymer Surface Interaction Studies
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- Tendon Structure and Treatment
- Axon Guidance and Neuronal Signaling
- Microtubule and mitosis dynamics
- Chemical Synthesis and Analysis
- Adhesion, Friction, and Surface Interactions
- Diatoms and Algae Research
- Algal biology and biofuel production
- Architecture and Computational Design
- Structural Analysis and Optimization
- Sports Dynamics and Biomechanics
- Caveolin-1 and cellular processes
- Nanopore and Nanochannel Transport Studies
- Advanced Sensor and Energy Harvesting Materials
- RNA modifications and cancer
- Proteoglycans and glycosaminoglycans research
- Proteins in Food Systems
- Lower Extremity Biomechanics and Pathologies
- Spectroscopy Techniques in Biomedical and Chemical Research
Harvard University
2022-2025
Harvard University Press
2024
University of California, Santa Barbara
2017-2021
Tufts University
2018
University of Massachusetts Amherst
2015
We present versatile tools for intersectional optical and chemical tagging of live cells. Photocaged tetrazines serve as "photo-click" adapters between recognition groups on the cell surface diverse payloads. describe two new functionalized photocaged tetrazine structures which add a light-gating step to three common cell-targeting methods: HaloTag/chloroalkane labeling, nonspecific primary amine antibody labeling. demonstrate light-gated versions these techniques in cultured then explore...
Significance Shape-programmable structures have recently used origami to reconfigure using a smooth folding motion, but are hampered by slow speeds and complicated material assembly. Inspired natural systems like the leaves of Venus flytraps hummingbird beaks, we use curved creases imbue elastic shells with programmable fast “snapping” motion. This deformation between preprogrammed states can be tuned either continuously foldable or snap discontinuously. Our results provide purely...
Neuronal axons must navigate a mechanically heterogeneous environment to reach their targets, but the biophysical mechanisms coupling mechanosensation, growth, and branching are not fully understood. Here, we show that local changes in membrane tension propagate along at approximately 20 μm/s, more than 1000-fold faster most other nonmotile cells where this property has been measured. Local perturbations decay axon with length constant of 41 μm. This rapid long-range mechanical signaling...
ABSTRACT Cancer cell fate has been widely ascribed to mutational changes within protein-coding genes associated with tumor suppressors and oncogenes. In contrast, the mechanisms through which biophysical properties of membrane lipids influence cancer survival, dedifferentiation metastasis have received little scrutiny. Here, we report that cells endowed a high metastatic ability stem cell-like traits employ ether maintain low tension fluidity. Using genetic approaches lipid reconstitution...
The configuration of charged polymers is heavily dependent on interactions with surrounding salt ions, typically manifesting as a sensitivity to the bulk ionic strength. Here, we use single-molecule mechanical measurements show that polysaccharide, hyaluronic acid, shows surprising regime insensitivity strength in presence trivalent ions. Using simulations and theory, propose this caused by formation "jacket" tightly associated polymer, whose charge (and thus effect configuration) robust...
We construct a mathematical model describing the equilibrium flotation height of spherical particle at interface immiscible liquids. The behavior such system depends on several experimentally measurable parameters, which include surface tensions, densities all phases, and scale. These parameters can be absorbed into three quantities that entirely determine position particle: contact angle between particle, Bond number, ratio buoyant density to liquid phase densities—a new, dimensionless...
The conformations of biological polyelectrolytes (PEs), such as polysaccharides, proteins, and nucleic acids, affect how they behave interact with other biomolecules. Relative to neutral polymers, PEs in solution are more locally rigid due intrachain electrostatic repulsion, the magnitude which depends on concentration added salt. This is typically quantified using Odijk-Skolnick-Fixman (OSF) electrostatic-stiffening model, salt-dependent Debye-H\"uckel (DH) screening modulates repulsion....
Abstract Neuronal axons must navigate a mechanically heterogeneous environment to reach their targets, but the biophysical mechanisms coupling mechanosensation, growth, and branching are not fully understood. Here, we show that local changes in membrane tension propagate along at approximately 20 µm/s, more than 1000-fold faster other non-motile cells. This rapid long-range mechanical signaling mediates bidirectional competition between axonal branch initiation growth cone extension. Our...
Single-molecule measurements of polymer elasticity are powerful, direct probes both biomolecular structure and principles physics. Recent work has revealed low-force regimes in which biopolymer is understood through blob-based scaling models. However, the small tensions required to observe these have potential create measurement biases, particularly due increased interactions chain with tethering surfaces. Here, we examine one experimentally observed bias, fluctuation-based estimates report...
Abstract We present versatile tools for intersectional optical and chemical tagging of live cells. Photocaged tetrazines serve as “photo-click” adapters between recognition groups on the cell surface diverse payloads. describe two new functionalized photocaged tetrazine structures which add a light-gating step to three common cell-targeting methods: HaloTag/chloroalkane labeling, nonspecific primary amine antibody labeling. demonstrate light-gated versions these techniques in cultured then...
Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and proteoglycan aggrecan contribute to cartilage compression resistance are necessary for healthy joint function. A variety of mechanical forces act on these in extracellular matrix, motivating need a quantitative description which links their structure response. Studies using electron microscopy have imaged HA-aggrecan brush but require adsorption surface, dramatically altering complex its native conformation. We...