A5031820791- Asthma and respiratory diseases
- Influenza Virus Research Studies
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Cellular transport and secretion
- Enzyme Structure and Function
- COVID-19 Clinical Research Studies
- Lysosomal Storage Disorders Research
- Ion Transport and Channel Regulation
- Virology and Viral Diseases
- Renal Diseases and Glomerulopathies
- Calcium signaling and nucleotide metabolism
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 and Mental Health
- Mass Spectrometry Techniques and Applications
- Food Safety and Hygiene
- Immune Response and Inflammation
- School Health and Nursing Education
- Celiac Disease Research and Management
- Respiratory and Cough-Related Research
- Inhalation and Respiratory Drug Delivery
University of Georgia
2014-2024
University of Arkansas for Medical Sciences
2016-2022
Duke University
2020-2021
Arkansas Children's Hospital
2016-2021
University of North Carolina at Chapel Hill
2017
Indiana University School of Medicine
2017
Arkansas Department of Agriculture
2014
University Gastroenterology
2013
Medical University of Vienna
2013
Dayanand Medical College & Hospital
2013
We describe an approach to accelerate the search for competitive inhibitors carbohydrate-recognition domains (CRDs). Genetically encoded fragment-based discovery (GE-FBD) uses selection of phage-displayed glycopeptides dock a glycan fragment at CRD and guide synergistic peptide motifs adjacent CRD. Starting from concanavalin A (ConA), mannose (Man)-binding protein, as bait, we narrowed library 108 86 leads that share consensus motif, Man-WYD. Validation synthetic yielded Man-WYDLF exhibited...
Abstract We report an integrated workflow that allows mass spectrometry-based high-resolution hydroxyl radical protein footprinting (HR-HRPF) measurements to accurately measure the absolute average solvent accessible surface area (<SASA>) of amino acid side chains. This approach is based on application multi-point HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement effective doses by dosimetry, and proper normalization inherent reactivity acids. The...
Abstract Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread around the globe at an unprecedented rate. To date, no effective therapeutic is available fight its associated disease, COVID-19. Our discovery of novel insertion glycosaminoglycan (GAG)-binding motif S1/S2 proteolytic cleavage site (681-686 (PRRARS)) two other GAG-binding-like motifs within SARS-CoV-2 spike glycoprotein (SGP) led us hypothesize that host cell...
Significance The activities of the fusion proteins that mediate virus–cell are an absolute requirement for virus entry and infectivity enveloped viruses such as HIV, influenza virus, measles respiratory syncytia among others. Viral translated initially in a metastable prefusion state and, upon triggering, undergo extensive irreversible refolding process. Membrane is coupled to energy released by adopting stable, low-energy postfusion state. Here we use oxidative footprinting parainfluenza 5...
Abstract The cation-independent mannose 6-phosphate receptor (CI-MPR, IGF2 or CD222), is a multifunctional glycoprotein required for normal development. Through the receptor’s ability to bind unrelated extracellular and intracellular ligands, it participates in numerous functions including protein trafficking, lysosomal biogenesis, regulation of cell growth. Clinically, endogenous CI-MPR delivers infused recombinant enzymes lysosomes treatment storage diseases. Although four 15 domains...
Protein–carbohydrate interactions are significant in a wide range of biological processes, disruption which has been implicated many different diseases. The capability glycan-binding proteins (GBPs) to specifically bind the corresponding glycans allows GBPs be utilized glycan biomarker detection or conversely serve as targets for therapeutic intervention. However, understanding structural origins GBP specificity proven challenging due their typically low binding affinities (mM) and potential...
We performed next-generation sequencing in patients with familial steroid-sensitive nephrotic syndrome (SSNS) and identified a homozygous segregating variant (p.H310Y) the gene encoding clavesin-1 (CLVS1) consanguineous family 3 affected individuals. Knockdown of clavesin zebrafish (clvs2) produced edema phenotypes due to disruption podocyte structure loss glomerular filtration barrier integrity that could be rescued by WT CLVS1 but not p.H310Y variant. Analysis cultured human podocytes...
Food allergy oral immunotherapy (OIT) has demonstrated efficacy in promoting clinically relevant immunomodulation that leads to desensitization (reduced reactivity while on OIT) the majority of treated individuals; however, sustained unresponsiveness after OIT cessation for a specified interval only been observed subset. The potential therapeutic benefits must be balanced with risk adverse events. These events may range from self-limited or easily oropharyngeal, respiratory, gastrointestinal...
Abstract We report an integrated workflow that allows mass spectrometry-based high-resolution hydroxyl radical protein footprinting (HR-HRPF) measurements to accurately measure the absolute average solvent accessible surface area (<SASA>) of amino acid side chains. This approach is based on application multi-point HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement effective doses by dosimetry, and proper normalization inherent reactivity acids. The...
The triggering receptor expressed on myeloid cells-2 (TREM2), a pivotal innate immune receptor, orchestrates functions such as inflammatory responses, phagocytosis, cell survival, and neuroprotection. TREM2 variants R47H R62H have been associated with Alzheimer's disease, yet the underlying mechanisms remain elusive. Our previous research established that binds to heparan sulfate (HS) exhibit reduced affinity for HS. Building upon this groundwork, our current study delves into interplay...
Abstract The adhesion of HIV gp120 antigen to human cells is modulated in part by interactions with heparan sulfate. HXB2 strain has been shown interact heparin primarily through the V3 loop, although other domains including C-terminal domain were also implicated. However, JR-FL (representative CCR5-interacting strains that make up newest infections) was have a drastically lowered affinity due loss several basic residues and deletion loop abrogate some, but not all, binding. Here, we use...
Abstract The cation-independent mannose 6-phosphate receptor (CI-MPR), also known as the IGF2 or CD222, is a multifunctional type I transmembrane glycoprotein ubiquitously expressed in most eukaryotic cell types. Through receptor’s ability to bind variety of unrelated extracellular and intracellular ligands, it involved wide array functions including protein trafficking, lysosomal biogenesis, internalization, regulation growth, migration apoptosis. CI-MPR has large region comprised 15...
The goal of this study was to gauge the natural history egg allergy in a population-based cohort and identify factors predicting persistence.Children (n = 264) determined be allergic raw eggs (according results skin testing oral food challenge) were recruited during February 2010 August 2011 from HealthNuts study, prospective, food-allergic children at age 12 months (N 5267) immunization sessions Melbourne, Australia.Egg-allergic infants offered baked challenge phenotype them as tolerant...