A5089295516- Pain Mechanisms and Treatments
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ion Channels and Receptors
- Stroke Rehabilitation and Recovery
- Spine and Intervertebral Disc Pathology
- Botulinum Toxin and Related Neurological Disorders
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Glioma Diagnosis and Treatment
- Pharmacological Receptor Mechanisms and Effects
- Balance, Gait, and Falls Prevention
- Treatment of Major Depression
- Menstrual Health and Disorders
- Peripheral Neuropathies and Disorders
- Vagus Nerve Stimulation Research
- Virus-based gene therapy research
- Neuroscience of respiration and sleep
- Peripheral Nerve Disorders
- Muscle activation and electromyography studies
- Asthma and respiratory diseases
- Anesthesia and Pain Management
- Viral Infections and Immunology Research
- Cancer Cells and Metastasis
Boston Children's Hospital
2013-2023
Stanford Health Care
2023
University of California, San Diego
2023
Colorado Permanente Medical Group
2023
Brigham and Women's Hospital
2019-2020
Harvard University
2013-2020
Stanford University
2020
MCPHS University
2019
University of Waterloo
2015
Background and Purpose Selective nociceptor fibre block is achieved by introducing the cell membrane impermeant sodium channel blocker lidocaine N‐ethyl bromide ( QX ‐314) through transient receptor potential V 1 TRPV1) channels into nociceptors. We screened local anaesthetics for their capacity to activate TRP channels, characterized nerve obtained combination with ‐314. Experimental Approach investigated activation in dorsal root ganglion DRG ) neurons calcium imaging patch‐clamp...
Although motion analysis is frequently employed in upper limb motor assessment (e.g. visually-guided reaching), they are resource-intensive and limited to laboratory settings. This study evaluated the reliability accuracy of a new markerless capture device, Leap Motion controller, measure finger position. Testing conditions that influence agreement between research-grade system were examined. Nine healthy young adults pointed 15 targets on computer screen under two conditions: (1) touching...
Glioblastoma multiforme (GBM) is a malignant brain tumor with poor prognosis resulting from resistance to anticancer therapy and high recurrence rate. Compelling evidence suggests that this driven by subpopulations of cancer stem cells (CSCs) tumor-initiating potential. ABC subfamily B member 5 (ABCB5) has been identified as molecular marker for distinct subsets chemoresistant cell populations in diverse human malignancies. In the current study, we examined potential role ABCB5 growth...
The corneal epithelium is renowned for high regenerative potential, which dependent on the coordinated function of its diverse progenitor subpopulations. However, molecular pathways governing epithelial differentiation are incompletely understood. Here, we identify a highly proliferative limbal subpopulation characterized by expression basal cell adhesion molecule (BCAM) that capable holocone formation and sheet generation. BCAM-positive cells can be found among ABCB5-positive stem (LSCs) as...
Pudendal neuropathy is a chronic, disabling form of perineal pain that involves the pudendal nerve, mixed somatic and autonomic nerve originates from sacral roots. Peripheral stimulation can be useful to decrease symptom burden in patients who have failed initial conservative treatment modalities.In this manuscript, we describe an approach placement peripheral stimulator for neuralgia. We present case complex pelvic review factors lead successful placement. Technical aspects ultrasound...
Complex orofacial pain disorders, such as trigeminal neuralgia (TN) and atypical facial (AFP), can be excruciating debilitating during attacks. Ketamine, an N-methyl-D-aspartate (NMDA) antagonist, is a powerful analgesic that has been used to treat various chronic conditions, but its role in treating complex only recently explored. In this retrospective case series, we reviewed the efficacy of continuous ketamine infusion for 12 patients with refractory medical treatment. Patients who...
BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal differentiation. Here, we present a protocol for isolating from human donor corneas by flow cytometry sorting. We describe steps dissection dissociation, antibody staining, cytometry. then detail procedures culturing the purified BCAM-negative sheet assays to study factors that regulate regeneration. For complete details on use execution this protocol,...