A5002388642- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Bacterial Genetics and Biotechnology
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Nuclear Structure and Function
- Cancer-related Molecular Pathways
- Physiological and biochemical adaptations
University of Dundee
2005-2024
University of St Andrews
2010-2012
Wellcome Trust
2005
The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in prokaryotic CRISPR system for antiviral defence. thought to play a role capture new viral DNA sequences incorporation into host genome. No biochemical activity has been reported Cas4, but it predicted include RecB nuclease domain. We show here that family from archaeon Sulfolobus solfataricus utilise four conserved cysteine residues bind an iron-sulfur cluster arrangement reminiscent AddB Bacillus subtilis....
For proper chromosome segregation in mitosis, sister kinetochores must interact with microtubules from opposite spindle poles (chromosome bi-orientation) [1Tanaka T.U. Kinetochore-microtubule interactions: steps towards bi-orientation.EMBO J. 2010; 29: 4070-4082Crossref PubMed Scopus (94) Google Scholar, 2Lampson M.A. Grishchuk E.L. Mechanisms to avoid and correct erroneous kinetochore-microtubule attachments.Biology (Basel). 2017; 6: 1-18Google Scholar]. To promote bi-orientation, Aurora B...
The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by kinases controls this entire process. One these controllers Aurora A kinase, which itself highly regulated. In study, we show nuclear pore protein ALADIN novel spatial regulator A. Without ALADIN, spreads from centrosomes onto microtubules, affects distribution subset microtubule regulators and slows assembly chromosome alignment. interacts with inactive recruited to pole...
This study investigated the expression and tissue distribution of inositol monophosphatase (IMPA1) characterized its role in salinity adaptation eel. The coding sequence eel IMPA1 was determined confirmed to be orthologous mammalian gene/enzyme by phylogenetic analysis structural modeling. Quantitative real-time PCR Western blot techniques indicated up 17-fold increases mRNA 2-fold protein abundance major osmoregulatory tissues following transfer fish seawater (SW). accompanied 5-fold enzyme...
To establish chromosome biorientation, aberrant kinetochore-microtubule interaction must be resolved (error correction) by Aurora B kinase. differentially regulates kinetochore attachment to the microtubule plus end and its lateral side (end-on attachment, respectively). However, it is still unclear how interactions are exchanged during error correction. Here, we reconstituted budding yeast interface in vitro attaching Ndc80 complexes nanobeads. These Ndc80C nanobeads recapitulated end-on...
Abstract For proper chromosome segregation, sister kinetochores must interact with microtubules from opposite spindle poles; this is called bi-orientation. To establish bi-orientation prior to any aberrant kinetochore–microtubule interaction be resolved (error correction) by Aurora B kinase that phosphorylates outer kinetochore components. differentially regulates attachment the microtubule plus end and its lateral side (end-on attachment, respectively). However, it still not fully...
To promote chromosome bi-orientation, Aurora B kinase weakens and disrupts aberrant kinetochore-MT interaction. It has long been debated how halts this action when bi-orientation is established tension applied across sister kinetochores. Pertinent to debate, it was shown that Bir1 (yeast Survivin), which recruits Ipl1-Sli15 B-INCENP) centromeres, dispensable for raising the possibility localization at centromeres not required bi-orientation. Here, we show COMA inner kinetochore sub-complex...