A5100345353- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Neuroscience and Neuropharmacology Research
- Galectins and Cancer Biology
- Glioma Diagnosis and Treatment
- Connexins and lens biology
- Epigenetics and DNA Methylation
- Chromatin Remodeling and Cancer
- melanin and skin pigmentation
- Carbohydrate Chemistry and Synthesis
- RNA regulation and disease
- Vibrio bacteria research studies
- Plant and Biological Electrophysiology Studies
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- Heat shock proteins research
- Ion Channels and Receptors
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
The University of Texas Southwestern Medical Center
2016-2024
Yan'an University
2022-2023
Shanghai East Hospital
2017-2023
Tongji University
2021-2023
Hebei Medical University
2016-2023
Affiliated Hospital of Guizhou Medical University
2022
Guiyang Medical University
2022
First Hospital of Shijiazhuang
2021
Zhengzhou Central Hospital
2020
The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in prokaryotic CRISPR system for antiviral defence. thought to play a role capture new viral DNA sequences incorporation into host genome. No biochemical activity has been reported Cas4, but it predicted include RecB nuclease domain. We show here that family from archaeon Sulfolobus solfataricus utilise four conserved cysteine residues bind an iron-sulfur cluster arrangement reminiscent AddB Bacillus subtilis....
Abstract The homeobox transcription factor Nanog has a vital role in maintaining pluripotency and self-renewal of embryonic stem cells (ESCs). Stabilization proteins is essential for ESCs. ubiquitin–proteasome pathway mediated by E3 ubiquitin ligases deubiquitylases one the key ways to regulate protein levels functions. Although ubiquitylation catalyzed ligase FBXW8 been demonstrated, deubiquitylase that maintains ESCs yet be defined. In this study, we identify ubiquitin-specific peptidase...
FANCM is branchpoint DNA translocase essential for cellular response to replication stress. Here, we show that stress stimulates and the TONSL-MMS22L heterodimer bound histones H3-H4 form an interdependent complex on chromatin. recruits Fanconi anemia (FA) core stalled collapsed forks, maintains replication-stressed chromatin, promotes FANCD2 monoubiquitination, facilitates both repair traverse of interstrand crosslinks (ICLs), suppresses sister chromatid exchanges, through its interactions...
AbstractObjectives: Our previous studies showed that delayed rectifier potassium currents existed in human gastric cancer cells and the were related to growth of cells. Human ether-a-go-go-related gene (herg) encoding α subunit channel has been indicated with involvement tumor cell death. The purpose present study is investigate expression HERG protein tissue cells; analyze relationship between clinicopathological characteristics patients cancer; explore effects on biological behaviours...
Type II epithelial-mesenchymal transition (EMT) plays a vital role in airway injury, repair, and remodeling. Triggered by growth factors, such as transforming factor beta (TGFβ), EMT induced biological process that converts epithelial cells into secretory mesenchymal with substantially increased production of extracellular matrix (ECM) proteins. Epithelial are not professional produce few ECM proteins under normal conditions. The molecular mechanism underlying the transformation protein...
Mucin glycans are important component of the mucus barrier and vital defence against physical chemical damage as well pathogens. Abnormal expression mucin can lead to disease, especially cancer. Here, we first summarize main types glycosylation on mucins mechanisms by which abnormal occur. We next describe role ab-normal in Finally, MUC1-based antibodies, vaccines, radio-pharmaceuticals, CAR-T therapies using best characterized MUC1 an example.
We report here the quantitative detection of Vibrio cholerae toxin (CT) in isolates and stool specimens by dynamic monitoring full course CT-mediated cytotoxicity a real-time cell analysis (RTCA) system. Four lines, including Y-1 mouse adrenal tumor cells, Chinese hamster ovary (CHO) small intestine epithelial (FHs74Int) gland (PC12-Adh) were evaluated for their suitability CT-induced testing. Among them, line was demonstrated to be most sensitive cytotoxicity, with limits 7.0 pg/ml purified...
Cardiomyocyte apoptosis induced by hypoxia and ischemia plays important roles in heart dysfunction after acute myocardial infarction (AMI). However, the mechanism of induction remains unclear. A previous study reported that Y-box protein 1 (YB1) is upregulated hypoxia/reoxygenation or ischemia/reperfusion (H/R I/R, respectively) injury; however, whether YB1 associated with H/R-induced cardiomyocyte completely unknown. In present study, we investigated possible underlying molecular...
Abstract Main Problem N‐acetylaspartylglutamate (NAAG) has neuroprotective effects in traumatic brain injury (TBI) by activating metabotropic glutamate receptor 3 (mGluR3) and reducing release. Glutamate carboxypeptidase II (GCPII) is the primary enzyme responsible for hydrolysis of NAAG. It remains unclear whether III (GCPIII), a homolog GCPII, can partially compensate GCPII's function. Methods GCPII −/− , GCPIII GCPII/III mice were generated using CRISPR/Cas9 technology. Mice model was...
Homeodomain transcription factor Nanog plays a critical role in maintaining murine embryonic stem (ES) cells pluripotency. However, its expression regulation largely remains unknown. In this study we show that Nogo receptor (NgR) participates the of via Stat3 pathway. Activation NgR results phosphorylation and increases levels mRNA protein, which inhibits differentiation embryoid bodies. This up-regulation can be abolished by inhibitor PI-PLC NEP1-40, or phospho-Stat3 AG490 rapamycin....
The accurate control of early cell fate specification during differentiation human embryonic stem cells (hESCs) is critical for acquiring pure therapeutic populations interest. Bone morphogenetic protein 4 (BMP4) a key mesoderm inducer from ESCs. However, the molecular mechanism mesodermal decision induced by BMP4 remains unclear. Here, we demonstrate requirement bioactive lipid, prostaglandin E2 (PGE2), hESCs induction. We show that directly regulates expression enzyme PGE2 synthesis,...
The rapid accumulation of single-cell RNA-seq data has provided rich resources to characterize various human cell populations. However, achieving accurate cell-type annotation using public references presents challenges due inconsistent annotations, batch effects, and rare types. Here, we introduce SELINA (single-cell identity navigator), an integrative automatic framework based on a pre-curated reference atlas spanning tissues. employs multiple-adversarial domain adaptation network remove...
In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived from the MESFs (CMESFs), monkey oviductal (MOFs), follicular granulosa fibroblast-like (MFGs) cells, epithelium-like (MFGEs) were developed for maintenance of rhesus embryonic stem cells (rESCs). We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support growth cells. Moreover, we detected some genes are upregulated in supportive lines by semi-quantitative PCR. present...
A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. pluripotency transgene-free iPSCs confirmed by immunocytochemistry for markers ability differentiate spontaneously into 3 germ layers in vitro. In addition, iPSC line displayed a normal karyotype. studies disease mechanism, can be as control.
Replisomes follow a schedule in which replication of DNA euchromatin is early S phase while sequences heterochromatin replicate late. Impediments to replication, referred as stress, can stall forks triggering activation the ATR kinase and downstream pathways. While there substantial literature on local consequences replisome stalling–double strand breaks, reversed forks, or genomic rearrangements–there limited understanding determinants stalling vs. continued progression. Although many...
Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed adult neurons. However, its function the dendritic growth of neurons hippocampus remains poorly understood. Here, we confirmed that Cic was hippocampal during main period dendritogenesis, suggesting has dendrite growth. Loss-of-function and gain-of assays indicated plays central role inhibition morphogenesis spines vitro . Further studies showed overexpression reduced...
Glioblastoma (GBM) is the most common aggressive primary malignant brain tumor, and patients with GBM have a median survival of 20 months. Clinical therapy resistance challenging barrier to overcome. Tumor genome stability maintenance during DNA replication, especially ability respond replication stress, highly correlated drug resistance. Recently, we identified protective role for RECQ1 under stress conditions. acts at forks, binds PCNA, inhibits single-strand formation nascent strand...
Baraitser-Winter Cerebrofrontofacial syndrome (BWCFF, OMIM: 243310) is a rare congenital developmental disorder marked by distinct facial dysmorphisms, coloboma, diminutive stature, and cognitive impairment, as initially described Baraitser Winter in 1988. Here, we derived human induced pluripotent stem cells (hiPSCs) from 4-year-old male patient diagnosed with harbouring mutation the ACTB gene. The newly established hiPSC line exhibited normal karyotypes demonstrated capacity to...