A5026108279- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Nanoparticles: synthesis and applications
- S100 Proteins and Annexins
- interferon and immune responses
- Atherosclerosis and Cardiovascular Diseases
- Environmental remediation with nanomaterials
- Protein purification and stability
- Virology and Viral Diseases
- SARS-CoV-2 detection and testing
- Advancements in Transdermal Drug Delivery
- Nanoparticle-Based Drug Delivery
- Respiratory viral infections research
- Heavy Metal Exposure and Toxicity
- Neonatal Respiratory Health Research
- Influenza Virus Research Studies
- Cervical Cancer and HPV Research
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
University of California, San Diego
2020-2023
Autonomous University of Aguascalientes
2017-2023
Due to the emergence of multi-drug resistant strains, development novel antibiotics has become a critical issue. One promising approach is use transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawback metals their toxicity eukaryotic Here, we show that barriers them as therapeutic agents could be mitigated by combining with silver. We demonstrate synergism combinatorial treatments (Silver/transition...
The COVID-19 pandemic highlights the need for platform technologies enabling rapid development of vaccines emerging viral diseases. current target SARS-CoV-2 spike (S) protein and thus far have shown tremendous efficacy. However, cold-chain distribution, a prime-boost administration schedule, emergence variants concern (VOCs) call diligence in novel vaccine approaches. We studied 13 peptide epitopes from identified three neutralizing that are highly conserved among VOCs. Monovalent trivalent...
Cowpea mosaic virus (CPMV) is a potent immunogenic adjuvant and epitope display platform for the development of vaccines against cancers infectious diseases, including coronavirus disease 2019. However, proteinaceous CPMV nanoparticles are rapidly degraded in vivo. Multiple doses therefore required to ensure long-lasting immunity, which not ideal global mass vaccination campaigns. Therefore, we formulated injectable hydrogels achieve slow particle release prolonged immunostimulation. Liquid...
Prophylactic vaccines capable of preventing human papillomavirus (HPV) infections are still inaccessible to a vast majority the global population due their high cost and challenges related multiple administrations performed in medical setting. In an effort improve distribution administration, we have developed dissolvable microneedles loaded with thermally stable HPV vaccine candidate consisting Qβ virus-like particles (VLPs) displaying highly conserved epitope from L2 protein (Qβ-HPV)....
Metastatic cancer accounts for 90% of all cancer-related deaths and continues to be one the toughest challenges in treatment. A growing body data indicates that S100A9, a major regulator inflammation, plays central role progression metastasis, particularly lungs, where S100A9 forms premetastatic niche. Thus, we developed vaccine against derived from plant viruses virus-like particles. Using multiple tumor mouse models, demonstrate effectiveness candidates preventing seeding within lungs...
Cardiovascular disease is the number one cause of death globally. Lowering cholesterol levels in plasma mainstay therapy; however lifelong treatment and adverse effects call for improved therapeutic interventions. We developed a trivalent vaccine candidate targeting proprotein convertase subtilisin/kexin-9 (PCSK9), apolipoprotein B (ApoB), cholesteryl ester transfer protein (CETP). Vaccine candidates were using bacteriophage Qβ-based virus-like particles (VLPs) displaying antigens PCKS9,...
The development of vaccines against coronaviruses has focused on the spike (S) protein, which is required for recognition host-cell receptors and thus elicits neutralizing antibodies. Targeting conserved epitopes S protein offers potential pan-beta-coronavirus that could prevent future pandemics. We displayed five B-cell epitopes, originally identified in convalescent sera from recovered severe acute respiratory syndrome (SARS) patients, surface cowpea mosaic virus (CPMV) evaluated these...
Human papillomavirus (HPV) is a globally prevalent sexually-transmitted pathogen, responsible for most cases of cervical cancer. HPV vaccination rates remain suboptimal, partly due to the need multiple doses, leading lack compliance and incomplete protection. To address drawbacks current vaccines, we used scalable manufacturing process prepare implantable polymer–protein blends single-administration with sustained delivery. Peptide epitopes from HPV16 capsid protein L2 were conjugated...
Ovarian cancer ranks fifth in deaths amongst women, and most patients are diagnosed with late-stage disseminated diseases. Surgical debulking chemotherapy remove of the tumor burden provide a short period remission; however, experience relapse eventually succumb to disease. Therefore, there is an urgent need for development vaccines prime anti-tumor immunity prevent its recurrence. Here we developed vaccine formulations composed mixture irradiated cells (ICCs, providing antigen) cowpea...
Abstract Our lab demonstrated that intratumoral Cowpea mosaic virus (CPMV) is a potent antitumor immunotherapy when used as in situ vaccine. As we pave the way for human clinical translation, formulation chemistry needs to be optimized long‐term storage of drug candidate. In this work, CPMV was nanoengineered with Pluronic F127 realize liquid and gel formulations which mitigate structural changes RNA release during storage. We evaluated CPMV‐F127 their stability biological activity through...
Due to the recent emergence of multi-drug resistant strains, development novel antimicrobial agents has become a critical issue. The use micronutrient transition metals is promising approach overcome this problem since these compounds exhibit significant toxicity at low concentrations in prokaryotic cells. In work, we demonstrate that lower than their minimal inhibitory and combination with different antibiotics, it possible mitigate barriers employ metallic micronutrients as therapeutic...
The standard therapy for cardiovascular disease (CVD) is the administration of statins to reduce plasma cholesterol levels, but this requires lifelong treatment. We developed a CVD vaccine candidate that targets pro-inflammatory mediator calprotectin by eliciting antibodies against S100A9 protein. vaccine, based on bacteriophage Qβ virus-like particles (VLPs) displaying peptide epitopes, was formulated as slow-release PLGA:VLP implant hot-melt extrusion. single-dose elicited S100A9-specific...
Conventional therapies and surgery remain the standard treatment for breast cancer. However, combating eventual development of metastasis is still a challenge. Newcastle disease virus (NDV) one various species viruses under clinical evaluation as vector oncolytic, gene-, immune-stimulating therapies. The purpose this study was to evaluate antitumor activity recombinant NDV (rNDV-P05) in cancer murine model.Tumors were induced by injecting cellular suspension (4T1 cell line) subcutaneously....
Atherosclerosis is a progressive cardiovascular disease in which cholesterol-rich plaques build up within arteries, increasing the risk of thrombosis, myocardial infarction, and stroke. One promising therapeutic approach use high-density lipoprotein (HDL) biomimetic formulations based on ApoAI peptides that promote cholesterol efflux from plaques, ultimately leading to excretion. Here, we describe multivalent display surface protein nanotubes derived plant virus tobacco mosaic (TMV)...
Antitumor conventional treatments including chemo/radiotherapy result in several side effects and non-specificity. Therapies the use of oncolytic viruses, particularly Newcastle disease virus (NDV), have emerged as an attractive alternative due to their capacity kill cancer cells directly or through stimulation immune system. In present study, a commercial vaccine composed recombinant attenuated NDV strain P05 (rNDV-P05) was assessed for antitumor immunostimulatory activity. Firstly,...
Abstract Plant virus nanoparticles are recognized as a platform technology for potential applications in nanomedicine. While non-infectious toward mammals, many plant by the immune system and act potent adjuvants, both classical setting of vaccination cancer immunotherapy. In context immunotherapy, we have demonstrated efficacy virus, particularly cowpea mosaic (CPMV) when applied intratumorally; CPMV signals through activates multiple toll-like receptors, which primes innate activation...