A5100688568- Extracellular vesicles in disease
- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Plant and Fungal Interactions Research
- Nanoparticles: synthesis and applications
- Bacterial biofilms and quorum sensing
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Gene expression and cancer classification
- Kruppel-like factors research
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
- Environmental remediation with nanomaterials
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Microbial Fuel Cells and Bioremediation
- Cell death mechanisms and regulation
- Heavy metals in environment
- Plant-Microbe Interactions and Immunity
- Cancer Research and Treatments
- Circular RNAs in diseases
- Transgenic Plants and Applications
- Genetic Syndromes and Imprinting
Dana-Farber Cancer Institute
2024
University of California, Riverside
2022-2023
University of California, Los Angeles
2023
Stanford University
2022
University of California, San Diego
2021
The University of Texas at Austin
2017-2021
National Sun Yat-sen University
2006
Summary Spray‐induced gene silencing (SIGS) is an innovative and eco‐friendly technology where topical application of pathogen gene‐targeting RNAs to plant material can enable disease control. SIGS applications remain limited because the instability RNA, which be rapidly degraded when exposed various environmental conditions. Inspired by natural mechanism cross‐kingdom RNAi through extracellular vesicle trafficking, we describe herein use artificial nanovesicles (AVs) for RNA encapsulation...
Small RNAs (sRNAs) of the fungal pathogen Botrytis cinerea can enter plant cells and hijack host Argonaute protein 1 (AGO1) to silence immunity genes. However, mechanism by which these sRNAs are secreted remains unclear. Here, we demonstrate that B. utilizes extracellular vesicles (EVs) secrete Bc-sRNAs, then internalized through clathrin-mediated endocytosis (CME). The tetraspanin protein, Punchless (BcPLS1), serves as an EV biomarker plays essential role in pathogenicity. We observe...
The COVID-19 pandemic highlights the need for platform technologies enabling rapid development of vaccines emerging viral diseases. current target SARS-CoV-2 spike (S) protein and thus far have shown tremendous efficacy. However, cold-chain distribution, a prime-boost administration schedule, emergence variants concern (VOCs) call diligence in novel vaccine approaches. We studied 13 peptide epitopes from identified three neutralizing that are highly conserved among VOCs. Monovalent trivalent...
One of the most promising tools for control fungal plant diseases is spray-induced gene silencing (SIGS). In SIGS, small interfering RNA (siRNA) or double-stranded (dsRNA) targeting essential virulence-related pathogen genes are exogenously applied to plants and postharvest products trigger interference (RNAi) targeted genes, inhibiting growth disease. However, SIGS limited by unstable nature under environmental conditions. The use layered double hydroxide clay particles as carriers deliver...
Extracellular vesicles (EVs) are membrane-enclosed nanometer-scale particles that transport biological materials such as RNAs, proteins, and metabolites. EVs have been discovered in nearly all kingdoms of life a form cellular communication across different cells between interacting organisms. EV research has primarily focused on EV-mediated intra-organismal mammals, which led to the characterization plethora contents from diverse cell types with distinct impactful physiological effects. In...
Extracellular vesicles (EVs) in plants have emerged as key players cell-to-cell communication and cross-kingdom RNAi between pathogens by facilitating the exchange of RNA, proteins, other molecules. In addition to their role intercellular communication, plant EVs also show promise potential therapeutics indicators health. However, exhibit significant heterogeneity protein markers, size, biogenesis pathways, strongly influencing composition functionality. While mammalian can be generally...
The biological synthesis of metal nanoparticles has been examined in a wide range organisms, due to increased interest green and environmental remediation applications involving heavy ion contamination.
Spray-Induced Gene Silencing (SIGS) is an innovative and eco-friendly technology where topical application of pathogen gene-targeting RNAs to plant material can enable disease control. SIGS applications remain limited because the instability dsRNA, which be rapidly degraded when exposed various environmental conditions. Inspired by natural mechanism cross-kingdom RNAi through extracellular vesicle trafficking, we describe herein use artificial nanovesicles (AVs) for dsRNA encapsulation...
Small RNAs (sRNAs) of the fungal pathogen Botrytis cinerea can enter plant cells and hijack host Argonaute protein 1 (AGO1) to silence immunity genes. However, mechanism by which these sRNAs are secreted remains unclear. Here, we demonstrate that B. utilizes extracellular vesicles (EVs) secrete Bc-sRNAs, then internalized through clathrin-mediated endocytosis (CME). The tetraspanin protein, Punchless (BcPLS1), serves as an EV biomarker plays essential role in pathogenicity. We observe...
Abstract It is known that Fas death domain‐associated protein (Daxx) possesses both putative nuclear and cytoplasmic functions. However, the transport mechanism largely unknown. This study examined location signal (NLS) of Daxx whether was mediated by small ubiquitin‐related modifier (SUMO). Two NLS motifs Daxx, leucine (L)‐rich export (NES)‐like motif ( 188 IXXLXXLLXL 197 ) C‐terminal lysine (K) rich 2 (amino acids 627–634) motif, were identified K 630 631 on characterized as major...
The tolerance of the extremophile, Deinococcus radiodurans , to numerous oxidative stresses makes it ideal for bioremediation applications, but many tools necessary metabolic engineering are lacking in this organism compared model bacteria. Although native and engineered promoters have been used drive gene expression protein production D. very few well characterized. Informed by bioinformatics, study expands repertoire well-characterized via thorough characterization eight putative with...
Genetic engineering of nanoparticle biosynthesis in bacteria could help facilitate the production nanoparticles with enhanced or desired properties. However, this process remains limited due to lack mechanistic knowledge regarding specific enzymes and other key biological factors. Herein, we report on ability small noncoding RNAs (sRNAs) affect silver (AgNP) using supernatant from bacterium Deinococcus radiodurans. Deletion strains 12 sRNAs potentially involved oxidative stress response were...
Abstract BACKGROUND The transcription factor PLAG1, ordinarily active during development, has been identified as overexpressed in several pediatric brain tumor entities, including H3 K27-altered diffuse midline glioma. Additionally, our group recently a novel type of embryonic CNS tumors with amplification the related genes PLAGL1 and PLAGL2. How this aberrant PLAG family gene activation drives formation is unknown. We hypothesize that activity these factors impedes normal differentiation,...
There is rising interest in nanoparticle biosynthesis using bacteria due to the potential for applications bioremediation, catalysis, or as antimicrobials. However, remains limited by inability control morphology and size lack of knowledge regarding explicit molecular mechanisms. Due their importance antimicrobials, we focus our discussion on silver, gold, copper nanoparticles. We discuss recent efforts elucidate reduction mechanisms that have identified generic enzymes metal resistance...