A5005654617- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant and Fungal Interactions Research
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Bioenergy crop production and management
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Phytoplasmas and Hemiptera pathogens
- Microtubule and mitosis dynamics
- Extracellular vesicles in disease
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Transgenic Plants and Applications
- Plant Diversity and Evolution
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
Nanjing Forestry University
2019-2024
State Key Laboratory of Tree Genetics and Breeding
2023
University of California, Riverside
2023
Zhejiang University
2022
Nanjing Agricultural University
2013-2020
Cotton Research Institute
2013-2020
University of North Carolina at Chapel Hill
2019
Institute of Vegetables and Flowers
2017
Chinese Academy of Agricultural Sciences
2017
Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, rearrangement, gene evolution and fiber biology. Upland is a model for polyploid crop domestication transgenic improvement. Here we sequenced allotetraploid hirsutum L. acc. TM-1 by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end genotype-by-sequencing genetic maps. We assembled annotated 32,032 A-subgenome genes 34,402 D-subgenome...
Allotetraploid cotton is an economically important natural-fiber-producing crop worldwide. After polyploidization, Gossypium hirsutum L. evolved to produce a higher fiber yield and better survive harsh environments than barbadense, which produces superior-quality fibers. The global genetic molecular bases for these interspecies divergences were unknown. Here we report high-quality de novo–assembled genomes two cultivated allotetraploid species with pronounced improvement in...
Cross-kingdom small RNA trafficking between hosts and microbes modulates gene expression in the interacting partners during infection. However, whether other RNAs are also transferred is unclear. Here, we discover that host plant Arabidopsis thaliana delivers mRNAs via extracellular vesicles (EVs) into fungal pathogen Botrytis cinerea. A fluorescent aptamer reporter Broccoli system reveals EVs recipient cells. Using translating ribosome affinity purification profiling polysome analysis,...
Summary Natural antisense transcripts ( NAT s) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved gene regulation plants. In this research, we investigated the function small RNA derived from fiber cell development. Using map‐based cloning strategy for first time tetraploid cotton, cloned naked seed mutant N 1 ) encoding MYBMIXTA ‐like transcription factor 3 MML )/ Gh MYB 25‐like chromosome A12, 3_A12 , that is...
Cotton, with cellulose-enriched mature fibers, is the largest source of natural textiles. Through a map-based cloning strategy, we isolated an industrially important lint fiber development gene (Li3 ) that encodes MYB-MIXTA-like transcription factor (MML) on chromosome D12 (GhMML4_D12). Virus-induced silencing or decreasing expression GhMML4_D12 in n2 NSM plants resulted significant reduction epidermal cell prominence and production. arranged tandem GhMML3, another MIXTA responsible for fuzz...
Abstract Background SNPs are the most abundant polymorphism type, and have been explored in many crop genomic studies, including rice maize. SNP discovery allotetraploid cotton genomes has lagged behind that of other crops due to their complexity polyploidy. In this study, genome-wide detected systematically using next-generation sequencing efficient genotyping methods, used construct a linkage map characterize structural variations polyploid genomes. Results We an ultra-dense inter-specific...
Cotton has been cultivated and used to make fabrics for at least 7000 years. Two allotetraploid species of great commercial importance, Gossypium hirsutum barbadense, were domesticated after polyploidization are worldwide. Although the overall genetic diversity between these two studied with limited accessions, their population structure variations remain largely unknown. We resequence genomes 147 cotton including diverse wild relatives, landraces, modern cultivars, construct a comprehensive...
Abstract Almost all plants in the genus Populus are dioecious (i.e. trees either male or female), but it is unknown whether dioecy evolved a common ancestor independently different subgenera. Here, we sequence small peritelomeric X- and Y-linked regions of P. deltoides chromosome XIX. Two genes present only region. One duplication non-Y-linked, female-specifically expressed response regulator, which produces siRNAs that block this gene’s expression, repressing femaleness. The other an...
Extracellular vesicles (EVs) are membrane-enclosed nanometer-scale particles that transport biological materials such as RNAs, proteins, and metabolites. EVs have been discovered in nearly all kingdoms of life a form cellular communication across different cells between interacting organisms. EV research has primarily focused on EV-mediated intra-organismal mammals, which led to the characterization plethora contents from diverse cell types with distinct impactful physiological effects. In...
The cotton fiber, as a single-celled trichome, is biological model system for studying cell differentiation and elongation. However, the complexity of gene expression regulation in fiber complicates genetic research. In this study, we investigated genome-wide transcriptome profiling Texas Marker-1 (TM-1) five naked seed or fuzzless mutants (three dominant two recessive) during fuzz initial development stage. More than three million clean tags were generated from each sample representing data...
To maintain tissue homeostasis, cells transition between cell cycle quiescence and proliferation. An essential G1 process is minichromosome maintenance complex (MCM) loading at DNA replication origins to prepare for S phase, known as origin licensing. A p53-dependent licensing checkpoint normally ensures sufficient MCM before phase entry. We used quantitative flow cytometry live imaging compare during the long first upon entry shorter phases in second subsequent cycles. discovered that...
Map-based gene cloning is a vital strategy for the identification of quantitative trait loci or genes underlying important agronomic traits. The conventional map-based method powerful but generally time-consuming and labor-intensive. In this context, we introduce an improved bulked segregant analysis in combination with virus-induced silencing (VIGS) rapid reliable mapping, functional verification. This was applied to multiple recessive marker line upland cotton, Texas 582 (T582), identified...
Expansins are a group of cell wall enzyme proteins that help to loosen walls by breaking hydrogen bonds between cellulose microfibrils and hemicellulose. essential plant involved in several key processes, including seed germination, the growth pollen tubes root hairs, fruit ripening abscission processes. Currently, there is lack knowledge concerning role expansins woody plants. In this study, we analyzed expansin genes using Populus genome as study target. Thirty-six members gene family were...
Although the CRISPR/Cas9 system has been widely used for crop breeding, its application genetic improvement of trees limited, partly because outcrossing nature and substantial genomic heterozygosity trees. Shanxin yang (Populus davidiana × P. bolleana), is a commercially important poplar clone that grown in northern China. An established transformation protocol this interspecific hybrid enables researchers to simultaneously investigate efficiency specificity CRISPR/Cas9-mediated manipulation...
In planta, a vital regulatory complex, MYB-basic helix-loop-helix (bHLH)-WD40 (MBW), is involved in trichome development and synthesis of anthocyanin proanthocyanin Arabidopsis. Usually, WD40 proteins provide scaffold for protein-protein interaction between MYB bHLH proteins. Members subgroup 9 the R2R3 transcription factors, which includes MYBMIXTA-Like (MML) genes important plant cell differentiation, are unable to interact with bHLH. this study, we report that cotton (Gossypium hirsutum)...
The MYB transcription factor (TF) family is one of the largest plant gene playing vital roles in growth and development, including defense, cell differentiation, secondary metabolism, responses to biotic abiotic stresses. As a model tree species woody plants, recent years, identification functional prediction certain members poplar genome have been reported. However, date, characterization poplar’s sister willow has not done, nor are differences similarities between genomes understood. In...
Salix wilsonii is an important ornamental willow tree widely distributed in China. In this study, integrated circular chloroplast genome was reconstructed for S. based on the reads screened from whole-genome sequencing data generated with PacBio RSII platform. The obtained pseudomolecule 155,750 bp long and had a typical quadripartite structure, comprising large single copy region (LSC, 84,638 bp) small (SSC, 16,282 separated by two inverted repeat regions (IR, 27,415 bp). encoded 115 unique...
Manipulation of genes controlling sex differentiation, flower development, and flowering in poplar is pivotal to shortening the juvenile phase for a speed breeding system or eliminate reduce dispersions polluting pollens hairy seeds. The sex-determining gene ( PtARR17 / PdFERR ) some core transcriptional regulators, such as MADS-box AGMOUS , have been identified Populus . However, interactions among them not explored well. Here, we integrated RNA-seq, small Bisulfite-seq characterize...
The morphology of cotton leaves varies considerably. Phenotypes, including okra, sea-island, super-okra, and broad leaf, are controlled by a multiple allele locus, L2 Okra leaf (L2°) is an incomplete mutation that alters shape increasing the length lobes with deeper sinuses. Using map-based cloning strategy, we cloned locus gene, which encodes LATE MERISTEM IDENTITY 1 (LMI1)-like transcription factor (GhOKRA). Silencing GhOKRA leads to change in phenotype from okra leaf. Overexpression...
Salix triandra belongs to section Amygdalinae in genus Salix, which is a different from the willow species sex determination has been well studied. Studying distantly related will help clarify whether sexes of willows arise through common system. For this purpose, we generated an intraspecific full-sib F1 population for S. and constructed high-density genetic linkage maps crossing parents using restriction site-associated DNA sequencing following two-way pseudo-testcross strategy. With...
Cotton is the source of most important, renewable natural textile fiber and oil in world. MicroRNAs (miRNAs) are endogenous, non-coding, approximately 18-24 nucleotides long RNAs function negative regulation their target genes. Two mostly overlapping libraries small RNA molecules were constructed sequenced, served as repetition sets data to identify miRNAs involved initiation seed development. The D genome sequence Gossypium raimondii was used conjunction with EST sequences predict miRNA...
Fasciclin-like arabinogalactan proteins (FLAs) are a subclass of (AGPs) containing both AGP-like glycated domains and fasciclin (FAS) domains, which involved in plant growth development synthesis the cell wall. However, these have not been identified or analyzed willow, Salix, sister genus Populus. In this study, we performed whole genome study FLA gene family Salix suchowensis compared it with Populus deltoides. The results showed presence 40 46 genes P. deltoides S. suchowensis,...
Summary Although many vector‐borne plant pathogens can alter vector behaviour to the pathogen's benefit, how plants might counter such manipulation is unknown. In Tomato yellow leaf curl virus (‘ TYLCV ’)– Bemisia tabaci –tomato interaction, ‐mediated changes in feeding improves viral uptake and transmission. We tested jasmonic acid JA ’), a central regulator of antiherbivore defences, affected ability (A) manipulate behaviour; (B) infect plants. Viruliferous fed much more than virus‐free...
Identification of the particular genes in an R supercluster underlying resistance to rust fungus Melampsora larici-populina poplar genome remains challenging. Based on de novo assembly Populus deltoides genome, all detected major genetic loci conferring M. were confined a 3.5-Mb region chromosome 19. The transcriptomes resistant and susceptible genotypes sequenced for timespan from 0 168 hours postinoculation. By mapping differentially expressed target genomic region, we identified two...