A5010277096- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Biochemical and biochemical processes
- Plant Pathogens and Fungal Diseases
- Plant Diversity and Evolution
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant-Microbe Interactions and Immunity
- Plant Genetic and Mutation Studies
- Bioenergy crop production and management
- Polysaccharides and Plant Cell Walls
- Forest Biomass Utilization and Management
- Lignin and Wood Chemistry
- interferon and immune responses
- Genetic Mapping and Diversity in Plants and Animals
- Oil Palm Production and Sustainability
Nanjing Forestry University
2018-2025
University of Georgia
2014-2025
Piedmont Athens Regional
2015-2024
State Key Laboratory of Tree Genetics and Breeding
2023-2024
Oak Ridge National Laboratory
2020
Sichuan University
2018
West China Hospital of Sichuan University
2018
Chinese Academy of Sciences
2008-2012
Shanghai Institutes for Biological Sciences
2008-2012
Center for Excellence in Molecular Plant Sciences
2008-2012
The genus Liriodendron belongs to the family Magnoliaceae, which resides within magnoliids, an early diverging lineage of Mesangiospermae. However, phylogenetic relationship magnoliids with eudicots and monocots has not been conclusively resolved thus remains be determined
Small RNAs (sRNAs) are common and effective modulators of gene expression in eukaryotic organisms. To characterize the sRNAs expressed during rice seed development, massively parallel signature sequencing (MPSS) was performed, resulting obtainment 797 399 22-nt sequence signatures, which 111 161 distinct ones. Analysis on distributions chromosomes showed that most originate from interspersed repeats mainly consist transposable elements, suggesting major function seeds is transposon...
Abstract Abscisic acid (ABA) regulates plant development and is crucial for responses to biotic abiotic stresses. Studies have identified the key components of ABA signaling in Arabidopsis (Arabidopsis thaliana), some which regulate by transcriptional regulation downstream genes. Here, we report functional identification rice (Oryza sativa) ABI5-Like1 (ABL1), a basic region/leucine zipper motif transcription factor. ABL1 expressed various tissues induced hormones indole-3-acetic stress...
Summary Poplar trees synthesize flavan‐3‐ols (catechin and proanthocyanidins) as a defense against foliar rust fungi, but the regulation of this response is poorly understood. Here, we investigated role hormones in regulating flavan‐3‐ol accumulation poplar during infection. We profiled levels hormones, signaling genes, metabolites black leaves at different stages Hormone were manipulated by external sprays, genetic engineering, drought to reveal their fungal defenses. Levels salicylic acid...
Abstract Almost all plants in the genus Populus are dioecious (i.e. trees either male or female), but it is unknown whether dioecy evolved a common ancestor independently different subgenera. Here, we sequence small peritelomeric X- and Y-linked regions of P. deltoides chromosome XIX. Two genes present only region. One duplication non-Y-linked, female-specifically expressed response regulator, which produces siRNAs that block this gene’s expression, repressing femaleness. The other an...
Background The development of rice (Oryza sativa) seed is closely associated with assimilates storage and plant yield, fine controlled by complex regulatory networks. Exhaustive transcriptome analysis developing embryo endosperm will help to characterize the genes possibly involved in regulation provide clues yield quality improvement. Principal Findings Our showed that metabolism regulation, hormone response cellular organization processes are predominantly expressed during development....
Salicylic acid (SA) has long been implicated in plant responses to oxidative stress. SA overproduction Arabidopsis thaliana leads dwarfism, making planta assessment of effects difficult this model system. We report that transgenic Populus tremula × alba expressing a bacterial synthase hyperaccumulated and conjugates without negative growth consequences. In the absence stress, endogenously elevated elicited widespread metabolic transcriptional changes resembled those wild-type plants exposed...
Sucrose transporters (SUTs) are essential for the export and efficient movement of sucrose from source leaves to sink organs in plants. The angiosperm SUT family was previously classified into three or four distinct groups, Types I, II (subgroup IIB) III, with dicot-specific Type I monocot-specific IIB functioning phloem loading. To shed light on underlying drivers evolution, Bayesian phylogenetic inference undertaken using 41 sequenced plant genomes, including seven basal lineages at key...
The lignin biosynthetic pathway is highly conserved in angiosperms, yet manipulations give rise to a variety of taxon-specific outcomes. Knockout lignin-associated 4-coumarate:CoA ligases (4CLs) herbaceous species mainly reduces guaiacyl (G) and enhances cell wall saccharification. Here we show that CRISPR-knockout 4CL1 poplar (Populus tremula × alba) preferentially reduced syringyl (S) lignin, with negligible effects on biomass recalcitrance. Concordant S-lignin was downregulation ferulate...
Redwood trees (Sequoioideae), including Metasequoia glyptostroboides (dawn redwood), Sequoiadendron giganteum (giant sequoia), and Sequoia sempervirens (coast are threatened widely recognized iconic tree species on the earth. The genomic resources of redwood would provide clues to their evolutionary relationship. Here, we report 8-Gb reference genome M. a comparative analysis with two relative species. More than 62% is composed repetitive sequences. clade-specific bursts long terminal repeat...
The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application biallelic mutations in stably transformed Populus, extending species range this powerful woody perennials. An underappreciated obstacle editing outcrossing frequent occurrence sequence polymorphisms that can render unproductive. discuss experimental evidence as well genome-wide computational analysis...
Plant tissue regeneration is critical for genetic transformation and genome editing techniques. During the process, changes in epigenetic modifications accompany cell fate transition. However, how allele-specific DNA methylation two haplotypes contributes to transcriptional dynamics during remains elusive. Here we applied an inter-species hybrid poplar (
Transgenic Populus tremula x alba (717-1B4) plants with reduced expression of a tonoplast sucrose efflux transporter, PtaSUT4, exhibit shoot growth compared to wild type (WT) under sustained mild drought. The present study was undertaken determine whether SUT4-RNAi directly or indirectly altered poplar predisposition and/or response changes in soil water availability. While and hexose levels were constitutively elevated organs, responses drought most the root tips plants. Prior any...
Abstract In Populus and many other tree species, photoassimilate sucrose diffuses down a concentration gradient via symplastically connected mesophyll cells to minor vein phloem for long‐distance transport. There is no evidence apoplastic phloem‐loading in . However, plasma membrane transporters (SUT1 SUT3) orthologous those associated with loading are expressed vascular tissues of poplar. While SUT3 functions import into developing xylem, the role SUT1 remains unclear. Here, we...
The success of CRISPR genome editing studies depends critically on the precision guide RNA (gRNA) design. Sequence polymorphisms in outcrossing tree species pose design hazards that can render ineffective. Despite recent advances sequencing with haplotype resolution, sequence polymorphism information remains largely inaccessible to various functional genomics research efforts. Populus VariantDB v3.2 addresses these challenges by providing a user-friendly search engine query heterozygous...
Manipulation of genes controlling sex differentiation, flower development, and flowering in poplar is pivotal to shortening the juvenile phase for a speed breeding system or eliminate reduce dispersions polluting pollens hairy seeds. The sex-determining gene ( PtARR17 / PdFERR ) some core transcriptional regulators, such as MADS-box AGMOUS , have been identified Populus . However, interactions among them not explored well. Here, we integrated RNA-seq, small Bisulfite-seq characterize...
Abstract In Arabidopsis (Arabidopsis thaliana), overproduction of salicylic acid (SA) increases disease resistance and abiotic stress tolerance but penalizes growth. This growth–defense trade-off has hindered the adoption SA-based management strategies in agriculture. However, investigation how SA inhibits plant growth been challenging because many SA-hyperaccumulating mutants have developmental defects due to pleiotropic effects underlying genes. Here, we heterologously expressed a...
Colletotrichum gloeosporioides is a hemibiotrophic pathogen causing significant losses to economically important crops and forest trees, including Liriodendron. To explore the interaction between C. Liriodendron identify candidate genes determining pathogenesis, we sequenced assembled whole genome of Lc1 (CgLc1) using PacBio Illumina next generation sequencing performed comparative genomic analysis CgLc1 Cg01, latter being described endophytic species complex. Gene structure prediction...
The CRISPR-Cas9 system has been deployed for precision mutagenesis in an ever-growing number of species, including agricultural crops and forest trees. Its application to closely linked genes with extremely high sequence similarities less explored. In this study, we used mutagenize a tandem array seven Nucleoredoxin1 (NRX1) spanning ∼100 kb Populus tremula × alba. We demonstrated efficient multiplex editing one single guide RNA 42 transgenic lines. mutation profiles ranged from small...
Cortical microtubules are integral to plant morphogenesis, cell wall synthesis, and stomatal behaviour, presumably by governing cellulose microfibril orientation. Genetic manipulation of tubulins often leads abnormal development, making it difficult probe additional roles cortical in biogenesis. Here, is shown that expressing post-translational C-terminal modification mimics α-tubulin altered characteristics guard dynamics transgenic Populus tremula x alba otherwise appear normal. 35S...