A5101724350- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Genetic and phenotypic traits in livestock
- Cellular Mechanics and Interactions
- Animal Behavior and Welfare Studies
- CRISPR and Genetic Engineering
- Hypothalamic control of reproductive hormones
- Agriculture and Biological Studies
- Animal Genetics and Reproduction
- Phytoestrogen effects and research
- Inflammatory mediators and NSAID effects
- Glycosylation and Glycoproteins Research
- Skin and Cellular Biology Research
- Bone health and osteoporosis research
- Hormonal and reproductive studies
- Pregnancy-related medical research
- Effects of Environmental Stressors on Livestock
- Heat shock proteins research
- Escherichia coli research studies
- Animal health and immunology
- Nonlinear Optical Materials Studies
- Alkaline Phosphatase Research Studies
- Growth Hormone and Insulin-like Growth Factors
Weizmann Institute of Science
1968-2022
Delft University of Technology
2022
Max Planck Institute for the Physics of Complex Systems
2017
Ministry of Agriculture and Rural Development
1988-2000
Omron (Japan)
1990
Cornell University
1975-1984
Hachaklait (Israel)
1981
Tel Aviv University
1974
Veterinary Research Institute
1971
Steroidal hormones produced by humans and animals are constantly excreted into the environment in their active forms. The primary steroid progesterone, estrone, estradiol, testosterone, cortisol, all of which lipophilic poorly soluble water. steroids major concern estrone estradiol-17β, since they exert physiological effects at a lower concentration than other can be found concentrations above LOEL for fish plants (10 ng/l). readily measured run-off, soil, groundwater, but each has its...
Scallops possess a visual system comprising up to 200 eyes, each containing concave mirror rather than lens focus light. The hierarchical organization of the multilayered is controlled for image formation, from component guanine crystals at nanoscale complex three-dimensional morphology millimeter level. layered structure tuned reflect wavelengths light penetrating scallop's habitat and tiled with mosaic square crystals, which reduces optical aberrations. forms images on double-layered...
Prostaglandins F (PGF) were measured in uterine vein, ovarian artery, and jugular vein plasma the endometrial tissues at various times during bovine estrous cycle, compared to peripheral progesterone levels. Four groups of heifers days 1-5, 10-14, 15-17 20-0 cycle studied. Low levels PGF (48 plus or minus 12 ng/g dry tissue endometrium on 1-14 cycle. Higher values (131 9.0) found 15 until day estrus (p less than 0.001). Similarly, very low observed (0.162) 0.044) ng/mlM S.E.), whereas ranged...
Progesterone levels in jugular venous blood are being measured by us cycling (unmated) and inseminated (pregnant or non-pregnant) cows as part of a comparative study with normal difficult breeding histories.Hawk, Wiltbank, Kidder & Casida (1955) reported high incidence embryonic death during the period 16 to 25 days after insemination.The 3rd week gestation may indeed be critical phase regulation luteal function, since towards end this regression corpus luteum normally occurs absence...
Mucus is made of enormous mucin glycoproteins that polymerize by disulfide crosslinking in the Golgi apparatus. QSOX1 a catalyst bond formation localized to Golgi. Both and mucins are highly expressed goblet cells mucosal tissues, leading hypothesis catalyzes disulfide-mediated polymerization. We found knockout mice lacking had impaired mucus barrier function due production defective mucus. However, an investigation on molecular level revealed normal polymerization related glycoproteins....
Transgenic bovine sperm were produced by restriction enzyme mediated insertion (REMI).REMI utilizes lipofection of linearized pEGFP and the corresponding for integration into genomic DNA.The transgenic used in IVF to produce morula expressing GFP.When AI two cows, resultant calves expressed exogenous DNA their lymphocytes as determined (a) PCR RT-PCR; (b) specific emission green fluorescence GFP; (c) Southern blot analysis.Data demonstrate that REMI is an efficient method production...
Dispersed bovine placental cells (fetal cotyledon and maternal caruncle) were shown to synthesize progesterone. To determine if their steroidogenic activity could be modulated by a cyclic nucleotide-mediated process, we added luteinizing hormone, 8-bromoadenosine 3',5'-monophosphate, 8-bromoguanosine adenosine, or cholera toxin dispersed from placentomes of 100-283 days gestational age examined progesterone synthesis during 3-to 16-hr incubation periods. Net production, defined as the amount...
Testosterone, progesterone, estradiol-17β, prostaglandin F (PGF) and E (PGE) secretion were measured by radioimmunoassay in cultured early, bovine fetal ovaries (n = 40) testes 62) from preimplantation age (32–35 days, 1.8–2.5 crown-rump, C-R length) postimplantation embryos (age 36–85 2.6–12.4 cm length). First measurable amounts of estradiol-17β secreted testosterone found gonads 3.3 length (approximately 42 days age). The range mean values the different groups for was 0.39–2.33...
SUMMARY Changes in the peripheral plasma level of oestradiol during bovine oestrous cycle were determined by a competitive protein-binding assay, following chromatographic purification steroid. Oestradiol concentration increased 3 days preceding oestrus and attained its peak value [17 ± 1·9 ( s.e.m. ) ng/100 ml] 4 h before behaviour could be detected. The declined steeply day to reach nadir (0·8 0·11) time ovulation. A minor rise was observed on more sustained increase 10–13, with 11 (8·1...
High-affinity LH/hCG binding sites have been characterized in porcine, lepine, and murine uteri. In the present study, LH/ hCG were bovine endometrium. Radioreceptor assays performed with membrane homogenates of endometrial tissues analyzed for site specificity capacity. There was little competition receptor occupancy between ovine FSH (5%) or prolactin (< 0.1% ), but there a 20% cross-reaction eCG. no affinity crude preparations kidney, skeletal muscle, vascular tissues. Concentrations...
Injections of arachidonic acid directly into the corpora lutea normal cattle at 12th or 13th day estrous cycle resulted in (a) a decline jugular vein plasma progesterone levels from an average 6 ng/ml to 2.5 (P < 0.05), (b) immediate rise PGF ovarian venous plasma, and (c) sharp estrogen levels. Jugular peaks were associated with each peak animal; LH did not change. These results show that bovine ovary can convert administered corpus luteum partial regression luteum. appears stimulate...
The hypothesis that FSH regulates the bovine cervical prostaglandin E2 (PGE2) synthesis is known to be associated with relaxation and opening at time of estrus was investigated. Cervical tissue from pre-estrous/estrous, luteal, postovulatory cows were examined for 1) presence (b) receptor (R) its corresponding mRNA 2) effect on PGE2 regulatory pathway in vitro. bFSHR cervix (maximal during pre-estrus/estrus) demonstrated by expression a reverse transcription (RT) polymerase chain reaction...
Summary. A rapid radiometric assay based on thin-layer-chromatography and the competition by progesterone for binding sites corticosterone-binding globulin of human plasma was used to determine changes in level peripheral bovine during oestrous cycle. The blood (ng/ml, mean ± S.E.) hormone minimal (0·6± 0·15 0·8± 0·20) from Day −2 +2 cycle (Day 0 = day oestrus), rose steeply between 3 7, either continued rise very slowly or maintained a plateau fluctuating about 5·4± 0·16 8 10 days, before...
Bone homeostasis is continuously regulated by the coordinated action of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between these two cell populations leads to pathological bone diseases such as osteoporosis osteopetrosis. Osteoclast functionality relies on formation sealing zone (SZ) rings that define resorption lacuna. It commonly assumed structure dynamic properties SZ depend physical chemical substrate. Considering unique complex native bone, elucidation relevant...
The presence of placental gonadotropin activity in early pregnancy was studied the cow. Cotyledons from first trimester gestation were used to isolate a chorionic gonadotropin-like (CG-like) protein by means ammonium sulfate precipitations (0.17-4.5 M) and gel filtration on Sephadex G-100 DEAE-Sephadex. CG-like measured radioreceptor assay (RRA) specific for human (hCG). Luteotrophic also assessed two separate vitro bioassays using cultures bovine granulosa rat Leydig cells. It found that 3...
Plasma and milk progesterone concentrations in pregnant sheep (18--22 days after mating) were similar, about 3.7 ng/ml whereas values non-pregnant less than 1 ng/ml. Lambing results indicated identical accuracy for both methods (82 84% 2 flocks). The was 92--100% ewes diagnosed the breeding season, but tested non-breeding season diagnosis of non-pregnancy according to levels only 50% accurate.
Bone remodeling relies on the coordinated functioning of osteoblasts, bone-forming cells, and osteoclasts, bone-resorbing cells. The effects specific chemical physical bone features osteoclast adhesive apparatus, sealing zone ring, their relation to resorption functionality are still not well-understood. We designed implemented a correlative imaging method that enables monitoring same area surface by time-lapse light microscopy, electron atomic force microscopy before, during, after exposure...