A5020455340- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Plant Virus Research Studies
- T-cell and Retrovirus Studies
- Influenza Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Respiratory viral infections research
- Cervical Cancer and HPV Research
- Microbial infections and disease research
- Veterinary Equine Medical Research
- Viral-associated cancers and disorders
- Animal Behavior and Welfare Studies
University of Bern
2008-2024
Federal Department of Home Affairs
2024
University of Zurich
2014-2023
Moredun Research Institute
2013-2020
Institute of Virology of the Slovak Academy of Sciences
2020
Friedrich-Loeffler-Institut
2019
Swiss Institute of Equine Medicine
2019
Agroscope
2019
Since 2016, A(H5Nx) high pathogenic avian influenza (HPAI) virus of clade 2.3.4.4b has become one the most serious global threats not only to wild and domestic birds, but also public health. In recent years, important changes in ecology, epidemiology, evolution this have been reported, with an unprecedented diffusion variety affected birds mammalian species. After two consecutive devastating epidemic waves Europe 2020-2021 2021-2022, second recognized as largest epidemics recorded so far,...
Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety sample types from patients with infectious syndromes. mNGS still its early stages broader translation into clinical applications. To further support the development, implementation, optimization and standardization procedures virus diagnostics, European Society Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The...
Metagenomic sequencing is increasingly being used in clinical settings for difficult to diagnose cases. The performance of viral metagenomic protocols relies a large extent on the bioinformatic analysis. In this study, European Society Clinical Virology (ESCV) Network NGS (ENNGS) initiated benchmark pipelines currently virological laboratories.Metagenomic datasets from 13 samples patients with encephalitis or respiratory infections characterized by PCR were selected. analyzed different...
Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle worldwide. Infection a pregnant animal may lead to persistent infection the foetus and birth persistently infected (PI) calf that sheds throughout its life. However, BVD viruses are not strictly species specific. BVDV has been isolated from many domesticated wild ruminants. This practical importance as reservoirs in non-bovine hosts hamper control cattle. A goat given social companion PI gave kid. In order...
Shotgun metagenomics using next generation sequencing (NGS) is a promising technique to analyze both DNA and RNA microbial material from patient samples. Mostly used in research setting, it now increasingly being the clinical realm as well, notably support diagnosis of viral infections, thereby calling for quality control implementation ring trials (RT) benchmark pipelines ensure comparable results. The Swiss NGS virology community therefore decided conduct RT 2018, order current metagenomic...
Equine parvovirus-hepatitis (EqPV-H) research is in its infancy. Information regarding prevalence, geographical distribution, genetic diversity, pathogenesis and risk factors enhances understanding of this potentially fatal infection.Determining the prevalence EqPV-H Austrian equids. Investigating increasing probability infection, liver-associated biochemistry parameters, concurrent equine hepacivirus (EqHV) infection phylogenetic analysis variants.Cross-sectional study.Sera from 259 horses...
The value of next-generation sequencing (NGS)-based applications for testing purposes in human medicine is widely recognized. Although NGS-based metagenomic screening may be interest veterinary medicine, particular intensively farmed livestock species such as pigs, there a lack protocols tailored to requirements, likely because the high diversity and samples. Therefore, we developed an protocol use virology present here different porcine medicine. To develop protocol, each step sample...
Influenza A viruses (IAV) are likely candidates for pandemics. This report summarizes the results of Swiss national program surveillance influenza in pigs and transmissions to humans between 2010 2022. Challenges optimization options discussed. Nasal swabs or lung tissue samples from with influenza-like signs (e.g. fever, cough) were screened by real-time RT-PCR swine virus (SIV) genomes, including that 2009 pandemic strain A(H1N1)pdm09; positive subtyped H1, N1, H3 N2 Sanger sequencing. In...
In Europe, highly pathogenic avian influenza (HPAI) virus circulates in wildlife, undergoing frequent reassortment, sporadic introductions domestic birds, and spillover to mammals. An H5N1 clade 2.3.4.4b reassortant, EA-2023-DG, affecting wild birds was detected western Europe November 2023. Six of its RNA segments came from the EA-2021-AB genotype, but polymerase basic 2 acidic originated low pathogenicity viruses. Discrete phylogeographic analyses concatenated genomes single suggested...
The purpose of this study was to investigate whether sheep grazing communal alpine pastures with cattle can transmit Border disease virus (BDV) cattle.A total 1170 and 923 were tested for BDV using RT-PCR (sheep) pestivirus antibodies an ELISA (cattle), respectively, before being moved one 4 (A, B, C D).Eight from pasture viraemic.396 examined the season seronegative.The latter re-examined after 99 seropositive or indeterminate.Antibody specificity determined in 25 these a serum...
As the causative agent of Infectious Bovine Rhinotracheitis (IBR) and Pustular Vulvovaginitis/Balanoposthitis (IPV/IPB), alphaherpesvirus 1 (BoHV-1) is responsible for high economic losses in cattle industry worldwide. This study aimed to establish a fast, colorimetric loop-mediated isothermal amplification (LAMP) assay detection viral DNA. Phenol red used as pH-sensitive readout, relying on distinct color change from pink yellow case positive reaction. LAMP reactions with different primers...
We present here the full-length genome sequences of two hepatitis E virus genotype 3 (HEV-3) isolates from a human stool sample patient with acute and raw sausage containing pig liver. Sequence analysis implies that Swiss HEV may form novel subgroup HEV-3 viruses.
The purpose of this study was to examine the occurrence sheep persistently infected with Border disease virus (BDV) on 76 mixed cattle and farms whether seroconversion BDV infection occurred in these farms.Seroprevalence bovine viral (BVDV) also investigated.Quantitative RT-PCR for pestivirus detection an ELISA detect antibodies were used 2'384 2'291 ovine blood samples, respectively.Another 27 seropositive from ten flocks underwent serum neutralization testing differentiate between BVDV...
Abstract Hepatitis E virus (HEV) is the causative agent of an acute and in most cases self‐limiting hepatitis. Of four major HEV genotypes that infect humans, genotype 3 4 are zoonotic have been identified humans but predominantly pigs wild boar, which considered main reservoirs. However, known host range may be increasing to comprise additional species, including companion animals. Several studies contact with dogs as a risk factor for infection yet information on occurrence Swiss lacking....
Bovine viral diarrhoea virus (BVDV) is an important pathogen of cattle that can naturally infect a wide range even-toed ungulates. Non-bovine hosts may represent reservoirs for the have potential to hamper BVDV eradication programs usually focused on cattle. Rabbits are very abundant in countries such as United Kingdom or Australia and often living near livestock pastures. Earlier reports indicated rabbits propagate upon intravenous exposure natural infection with occur but experimental...
Cattle persistently infected with a noncytopathic Bovine viral diarrhea virus (BVDV) are at risk of developing fatal "mucosal disease" (MD). The authors investigated the role various apoptosis pathways in pathogenesis lesions animals suffering from MD. Therefore, they compared expression caspase-3, caspase-8, caspase-9, and Bcl-2L1 (Bcl-x) tissues 6 BVDV-free control animals, 7 (PI) that showed no signs MD (non-MD PI animals), 11 correlated staining localization mucosal lesions. Caspase-3 -9...