Michael Huber
A5062903597- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- HIV-related health complications and treatments
- Viral Infections and Immunology Research
- Infection Control and Ventilation
- T-cell and B-cell Immunology
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- COVID-19 epidemiological studies
- interferon and immune responses
- Infection Control in Healthcare
- Pneumocystis jirovecii pneumonia detection and treatment
- Virus-based gene therapy research
- Hepatitis C virus research
University of Zurich
2016-2025
RWTH Aachen University
2015-2025
University Hospital of Zurich
2006-2024
Inserm
2024
University of Lausanne
2019-2024
Université Claude Bernard Lyon 1
2024
University of St. Gallen
2023-2024
University of Bern
2017-2023
University of Basel
2018-2023
University Hospital Regensburg
1997-2023
The membrane-proximal external region (MPER) of HIV-1, located at the C terminus gp41 ectodomain, is conserved and crucial for viral fusion. Three broadly neutralizing monoclonal antibodies (bnMAbs), 2F5, 4E10, Z13e1, are directed against linear epitopes mapped to MPER, making this an important potential vaccine target. However, no MPER have been definitively shown provide protection HIV challenge. Here, we show that both MAbs 2F5 4E10 can complete mucosal simian-human immunodeficiency virus...
Repurposing existing, clinically approved, antiviral drugs as COVID-19 therapeutics is a rapid way to help combat the SARS-CoV-2 pandemic. Interferons (IFNs) usually form part of body’s natural innate immune defenses against viruses, and they have been used with partial success treat previous new viral threats, such HIV, hepatitis C virus, Ebola virus. Nevertheless, IFNs can undesirable side effects, recent reports indicate that upregulate expression host ACE2 (a critical entry receptor for...
Abstract The HIV-1 reservoir is the major hurdle to a cure. We here evaluate viral and host characteristics associated with size long-term dynamics in 1,057 individuals on suppressive antiretroviral therapy for median of 5.4 years. At population level, decreases diminishing differences over time, but increases 26.6% individuals. Viral blips low-level viremia are significantly slower decay. Initiation ART within first year infection, pretreatment load, ethnicity affect size, less so dynamics....
Background and Purpose: Case series indicating cerebrovascular disorders in coronavirus disease 2019 (COVID-19) have been published. Comprehensive workups, including clinical characteristics, laboratory, electroencephalography, neuroimaging, cerebrospinal fluid findings, are needed to understand the mechanisms. Methods: We evaluated 32 consecutive critically ill patients with COVID-19 treated at a tertiary care center from March 9 April 3, 2020, for concomitant severe central nervous system...
To examine longitudinal changes in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence and to determine the clustering of children who were seropositive within school classes canton Zurich, Switzerland from June November 2020.Prospective cohort study.Switzerland had one highest second waves SARS-CoV-2 pandemic Europe autumn 2020. Keeping schools open provided a moderate high exposure environment study infections. Children randomly selected classes, stratified by...
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in variety sample types and infectious syndromes. mHTS still its early stages translating into clinical application. To support development, implementation standardization procedures virus diagnostics, European Society Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim ENNGS to bring together...
Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety sample types from patients with infectious syndromes. mNGS still its early stages broader translation into clinical applications. To further support the development, implementation, optimization and standardization procedures virus diagnostics, European Society Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The...
Abstract Background Advancements in access to antiretroviral therapy (ART) and human immunodeficiency virus (HIV) care have led a decline AIDS-related deaths among people with HIV (PWH) Switzerland. However, data on the ongoing changes causes of death PWH over past 15 years are scarce. Methods We investigated all reported Swiss Cohort Study between 2005 2022. Causes were categorized using Coding Death protocol. The statistical analysis included demographic stratification identify time trends...
Autoantibodies neutralizing type I interferons (IFN-Is) can underlie infection severity. Here, we trace the development of these autoantibodies at high-resolution using longitudinal samples from 1,876 well-treated individuals living with HIV over a 35-year period. Similar to general populations, ∼1.9% acquired anti-IFN-I as they aged (median onset ∼63 years). Once detected, persisted lifelong, and titers increased decades. Individuals developed distinct non-neutralizing autoantibody...
Identifying HIV-1 envelope (Env) traits associated with neutralization cross-reactivity is crucial for vaccine design. Variable loops 1 and 2 (V1V2), positioned at the Env trimer apex, are key regions linked to neutralization. We describe non-canonical cysteine (Cys) residues in V1 that enriched individuals elite breadth. Analyzing over 65,000 sequences from CATNAP database, AMP trials, longitudinal cohorts (SHCS, ZPHI, CAPRISA), we found variants extra Cys present low levels fluctuate time....
Abstract Mast cells develop from the myeloid lineage and are released bone marrow as immature cells, which then differentiate at destination tissue based on cues local environment. In liver, mast recruited in diseased states to fibrogenic surroundings rich TGF-β1. The aim of this study was investigate TGF-β1 signaling primary permanent identify common unique mechanisms. TGF-β receptor repertoire is similar among with high expression type I II receptors very low III (Betaglycan Endoglin)....
Background To explore the possibility that antibody-mediated complement lysis contributes to viremia control in HIV-1 infection, we measured activity of patient plasma mediating autologous primary virus. Methods and Findings Sera from two groups patients—25 with acute infection 31 chronic infection—were used this study. We developed a novel real-time PCR-based assay strategy allows reliable sensitive quantification virus by complement. Plasma derived at time isolation induced isolate...
Antiretroviral therapy has transformed HIV infection from a deadly into chronic condition. Aging people with (PWH) are at higher risk of polypharmacy, potential drug-drug interactions (DDIs), and potentially inappropriate medications (PIMs). This study aims to compare prescribed drugs, DDIs between young (<65 years old) elderly (≥65 PWH. The prevalence PIMs was assessed in elderly.PWH 2 centers within the Swiss Cohort Study were asked fill form all their current medications. Polypharmacy...
Metagenomic sequencing is increasingly being used in clinical settings for difficult to diagnose cases. The performance of viral metagenomic protocols relies a large extent on the bioinformatic analysis. In this study, European Society Clinical Virology (ESCV) Network NGS (ENNGS) initiated benchmark pipelines currently virological laboratories.Metagenomic datasets from 13 samples patients with encephalitis or respiratory infections characterized by PCR were selected. analyzed different...
Since entering the human population, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2; causative agent of Disease 2019 [COVID-19]) has spread worldwide, causing >100 million infections and >2 deaths. While large-scale sequencing efforts have identified numerous genetic variants in SARS-CoV-2 during its circulation, it remains largely unclear whether many these changes impact adaptation, replication, or transmission virus. Here, we characterized 14 different low-passage...
Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) its collection is simple, non-invasive amenable mass- home testing, but rigorous validation, particularly in children, missing. We conducted a large-scale head-to-head comparison of detection by RT-PCR saliva NPS 1270 adults children reporting outpatient centers emergency unit. In total, 273 individuals were tested positive...
Abstract Determination of SARS-CoV-2 antibody responses in the context pre-existing immunity to circulating human coronavirus (HCoV) is critical for understanding protective immunity. Here we perform a multifactorial analysis and HCoV pre-pandemic ( N = 825) SARS-CoV-2-infected donors 389) using custom-designed multiplex ABCORA assay. seroprofiling, when combined with computational modeling, enables accurate definition seroconversion prediction neutralization activity, reveals intriguing...
BACKGROUND. Neutralizing antibodies are considered a key correlate of protection by current SARS-CoV-2 vaccines. The manner in which human infections respond to therapeutic antibodies, including convalescent plasma therapy, remains be fully elucidated.
Genome sequences from evolving infectious pathogens allow quantification of case introductions and local transmission dynamics. We sequenced 11,357 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes Switzerland in 2020-the sixth largest effort globally. Using a representative subset these data, we estimated viral to their persistence over the course 2020. contrasted estimates with simple null models representing absence certain public health measures. show that...