A5020589396- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Eosinophilic Disorders and Syndromes
- Cervical Cancer and HPV Research
- Plant Virus Research Studies
- Vaccine Coverage and Hesitancy
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Nuclear Structure and Function
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Genital Health and Disease
- Cancer Genomics and Diagnostics
- Viral Infections and Immunology Research
- Pneumonia and Respiratory Infections
- Emergency and Acute Care Studies
- CRISPR and Genetic Engineering
- Ion channel regulation and function
- Tumors and Oncological Cases
- Polyomavirus and related diseases
- Viral gastroenteritis research and epidemiology
- bioluminescence and chemiluminescence research
- Virology and Viral Diseases
- Retinoids in leukemia and cellular processes
In December 2020, the United Kingdom (UK) reported a SARS-CoV-2 Variant of Concern (VoC) which is now named B.1.1.7. Based on initial data from UK and later other countries, this variant was estimated to have transmission fitness advantage around 40-80 % (Volz et al., 2021; Leung Davies 2021).
Genome sequences from evolving infectious pathogens allow quantification of case introductions and local transmission dynamics. We sequenced 11,357 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes Switzerland in 2020-the sixth largest effort globally. Using a representative subset these data, we estimated viral to their persistence over the course 2020. contrasted estimates with simple null models representing absence certain public health measures. show that...
The rapid spread of the SARS-CoV-2 lineages B.1.1.7 (N501Y.V1) throughout UK, B.1.351 (N501Y.V2) in South Africa, and P.1 (B.1.1.28.1; N501Y.V3) Brazil has led to definition variants concern (VoCs) recommendations for lineage specific surveillance. In Switzerland, during last weeks December 2020, we established a nationwide screening protocol across multiple laboratories, focusing first on epidemiological microbiological definitions. January 2021, validated implemented an N501Y-specific PCR...
The number of predictive biomarkers that will be necessary to assess in clinical practice increase with the availability drugs target specific molecular alterations. Therefore, diagnostic laboratories are confronted new challenges: costs, turn-around-time and amount material required for testing tests performed on a sample. Our consortium European research set out test if semi-conductor sequencing provides solution these challenges. We designed multiplex PCR targeting 87 hotspot regions 22...
In a double-blind, randomised, placebo-controlled trial of hospitalised patients with community-acquired pneumonia (CAP), we demonstrated shorter time to clinical stability (TTCS) adjunct corticosteroid therapy compared placebo. We did pre-planned, exploratory analysis any association between microbiological diagnosis, antibiotic treatment and procalcitonin level effect prednisone on TTCS, mortality, CAP complications (n=726 participants, enrolled December 2009 May 2014). Multiplex viral...
Accumulating evidence regarding the causative role of human papillomavirus (HPV) in a wide range malignant and nonmalignant diseases highlights importance HPV testing. This study describes discusses efficacy characteristics 4 well-established commercially available tests. Here, 181 cytologic specimens from cervical smears were analyzed using SIGN PQ (Diatech) Linear Array (Roche) method. Discrepant results further studied with Real Time High-Risk (Abbott) method INNO-LiPA (Fujirebio) Of...
Abstract SARS-CoV-2, the virus responsible for current COVID-19 pandemic, is evolving into different genetic variants by accumulating mutations as it spreads globally. In addition to this diversity of consensus genomes across patients, RNA viruses can also display within individual hosts, and co-existing viral may affect disease progression success medical interventions. To systematically examine intra-patient we processed a large cohort 3939 publicly-available deeply sequenced with...
Hutchinson-Gilford progeria syndrome (HGPS) is a genetic disorder displaying features reminiscent of premature senescence caused by germline mutations in the LMNA gene encoding lamin A and C, essential components nuclear lamina. By studying family with homozygous mutation (K542N), we showed that HGPS can also be affecting both isoforms, C. Here, aimed to elucidate molecular mechanisms underlying pathogenesis both, A- (sporadic) C-related (hereditary) HGPS. For this, performed detailed...
Abstract Pathogen genomes provide insights into their evolution and epidemic spread. We sequenced 1,439 SARS-CoV-2 from Switzerland, representing 3-7% of all confirmed cases per week. Using these data, we demonstrate that no one lineage became dominant, pointing against towards general lower virulence. On an epidemiological level, report evidence cryptic transmission before the first case. find many early viral introductions Germany, France, Italy recent Germany France. Over summer, quantify...
Abstract Background In December 2020, the United Kingdom (UK) reported a SARS-CoV-2 Variant of Concern (VoC) which is now named B.1.1.7. Based on initial data from UK and later other countries, this variant was estimated to have transmission fitness advantage around 40-80% [1, 2, 3]. Aim This study aims estimate effective reproductive number B.1.1.7 through time based Switzerland. Methods We generated whole genome sequences 11.8% all confirmed cases in Switzerland between 14 2020 11 March...
Abstract Genome sequences from evolving infectious pathogens allow quantification of case introductions and local transmission dynamics. We sequenced 11,357 SARS-CoV-2 genomes Switzerland in 2020 - the 6th largest effort globally. Using a representative subset these data, we estimated viral to their persistence over course 2020. contrast estimates with simple null models representing absence certain public health measures. show that Switzerland’s border closures de-coupled incidence...
Abstract The rapid spread of the SARS-CoV-2 lineages B.1.1.7 (N501Y.V1) throughout UK, B.1.351 (N501Y.V2) in South Africa, and P.1 (B.1.1.28.1; N501Y.V3) Brazil has led to definition variants concern (VoCs) recommendations by European Center for Disease Prevention Control (ECDC) World Health Organization (WHO) lineage specific surveillance. In Switzerland, during last weeks December 2020, we established a nationwide screening protocol across multiple laboratories, focusing first on...
Background Many current methods for the detection of gene variants relevant inherited disorders like malignant hyperthermia (MH) rely on polymerase chain reaction (PCR). A positive PCR result genetic region interest is required downstream analysis sequencing, restriction digestion or probe hybridization. However, it sometimes overlooked that in order to obtain a true genotype, both paternal and maternal alleles must be represented product. We present case preferential amplification one...
Abstract Low‐dose interferon‐α 2a treatment may be considered as an alternative to cytoreductive therapy with hydroxyurea or regularly dosed interferon in high‐risk polycythemia vera patients.
Early treatment of polycythemia vera with ultra-low-dose interferon-α 2a is well tolerated and results in complete hematologic major molecular remission a strong reduction all symptoms, especially pruritus fatigue.