A5010989324- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Micro and Nano Robotics
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Lattice Boltzmann Simulation Studies
- Material Dynamics and Properties
- Ionic liquids properties and applications
- Spectroscopy and Quantum Chemical Studies
- Cell Adhesion Molecules Research
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- 3D Printing in Biomedical Research
- Advanced Thermodynamics and Statistical Mechanics
- Electrostatics and Colloid Interactions
- Characterization and Applications of Magnetic Nanoparticles
- Phase Equilibria and Thermodynamics
- Pickering emulsions and particle stabilization
- Theoretical and Computational Physics
- NMR spectroscopy and applications
- Epoxy Resin Curing Processes
- Electrochemical Analysis and Applications
- Catalytic Processes in Materials Science
- Crystallization and Solubility Studies
- Asymmetric Hydrogenation and Catalysis
Rudjer Boskovic Institute
2015-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2024
Instituto de Física Teórica
2014-2024
University of Zagreb
2014-2023
Cornell University
2021
Center for Theoretical Physics
2020
Eindhoven University of Technology
2019
Bar-Ilan University
2019
Southern Cross University
2014
University of Stuttgart
2005-2011
Abstract Partitioning space into cells with certain extreme geometrical properties is a central problem in many fields of science and technology. Here we investigate the Quantizer problem, defined as optimisation moment inertia Voronoi cells, i.e., similarly-sized ‘sphere-like’ polyhedra that tile are preferred. We employ Lloyd’s centroidal diagram algorithm to solve this find it converges disordered states associated deep local minima. These universal sense their structure factors...
In living cells, adhesion structures have the astonishing ability to grow and strengthen under force. Despite rising evidence of importance this phenomenon, little is known about underlying mechanism. Here, we show that force-induced adhesion-strengthening can occur purely because thermodynamic response elastic deformation membrane, even in absence actively regulated cytoskeleton cell, which was hitherto deemed necessary. We impose pN-forces on two fluid membranes, locally pre-adhered by...
It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description such cell tiling. particularly useful approach so called Voronoi tessellation, built from centers mass nuclei (CMVT), which commonly used for estimating morphology epithelial tissues. However, a study providing statistically sound analysis this method's accuracy not available literature. We addressed issue here by...
The Stokes-Einstein-Sutherland (SES) equation is at the foundation of statistical physics, relating a particle's diffusion coefficient and size with fluid viscosity, temperature, boundary condition for particle-solvent interface. It assumed that it relies on separation scales between particle solvent, hence expected to break down diffusive transport molecular scale. This assumption however challenged by number experimental studies showing remarkably small, if any, violation, while...
We present a comprehensive model for the nucleation of domains in membrane adhesion. determine critical number bonds nucleus and calculate probability distribution time from discrete master equation. The latter is characterized by only four effective rates, which account cooperative effects between bonds. validate our finding excellent agreement with extensive Langevin simulations. In range parameters typical cell adhesion, we find to be small. Furthermore, characteristic separation at...
Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack tools accurately record them. Here we introduce novel technique--dynamic optical displacement spectroscopy (DODS), measure stochastic with unprecedented combined spatiotemporal resolution 20 nm and 10 μs. The technique validated by measuring bending model membranes....
We present a synergistic combination of simulations and experimental data on the dynamics membrane adhesion. show that change in either density or strength bonds results very different dynamics. Such behavior is explained by introducing an effective binding affinity emerges as result competition between chemical environment defined fluctuating membrane.
Dendritic cells (DCs) are professional antigen-presenting of the immune system. Upon sensing pathogenic material in their environment, DCs start to mature, which includes cellular processes, such as antigen uptake, processing and presentation, well upregulation costimulatory molecules cytokine secretion. During maturation, detach from peripheral tissues, migrate nearest lymph node, find way into correct position net node microenvironment meet interact with respective T cells. We hypothesize...
Despite the primary role of cell proliferation in tissue development and homeostatic maintenance, interplay between density, mechanoresponse, growth division is not yet understood. In this article, we address issue by reporting on an experimental investigation all time- length-scales a model tissue, grown collagen-coated glass or deformable substrates. Through extensive data analysis, demonstrate relation mechanoresponse probability for division, as function local density. Motivated these...
The competition between adhesion and tether formation in bound vesicles is investigated. A theoretical model developed which tethers are induced by the application of a pulling force to top strongly adhered vesicle. critical onset identified where spontaneously appears as part first order shape transition. Further growth initiates detachment process that culminates continuous unbinding vesicle at finite force. Both forces, well all parameters, calculated function reduced volume strength...
Giant unilamellar vesicles (GUVs) adhering to supported bilayers were used as a model system mimic ligand–receptor mediated cell-cell adhesion. We present the effect of varying concentration receptors (neutravidin on bilayer) and ligands (biotin vesicle) GUV adhesion organization in zone. At high concentrations both receptors, is strong, all available membrane adhered are accumulated under up geometrical limit close packing. low (<0.5%), an arbitrary (≥0.1%), does not proceed completion:...
Understanding the molecular-level behavior of ionic liquids (ILs) at IL–solid interfaces is fundamental importance with respect to their application in, for example, electrochemical systems and electronic devices. Using a model system, consisting an imidazolium-based IL ([C2Mim][NTf2]) in contact sapphire substrate, we have approached this problem using complementary combination high-resolution X-ray reflectivity measurements atomistic molecular dynamics (MD) simulations. Our strategy...
In this analytical study we demonstrate the richness of behaviour exhibited by bead-spring micro-swimmers, both in terms known yet not fully explained effects such as synchronisation, and hitherto undiscovered phenomena existence two transport regimes where swimmer shape has fundamentally different on velocity. For purpose employ a micro-swimmer model composed three arbitrarily-shaped rigid beads connected linearly springs. By analysing forces beads, determine optimal kinematic parameters...
A theoretical approach has been undertaken in order to model the thermodynamic equilibrium of a 3D vesicle adhering flat substrate. The is treated canonical description with fixed number sites. finite these sites are occupied by mobile ligands that capable interacting discrete receptors immobilized on Explicit consideration bending energy shape shown problem can be decoupled from determination optimum allocation over vesicle. bound and free determined as function size contact zone,...
Prior to establishing tight contact with the endothelium, cells such as leukocytes or cancer use recognition between sialyl-LewisX ligands and E-selectin receptors establish weak, reversible adhesion roll along vessel wall. We study physical aspects of this process by constructing a mimetic system that consists giant fluid vesicle incorporated lipid-anchored molecules bind is immobilized on flat substrate. The vesicles also carry certain fraction repelling PEG2000 molecules. analyze...
Patchy particles comprise regions of differing material or chemical functionality on otherwise isotropic cores. To meet the great potential these anisotropic structures in a wide range application fields, completely new approaches are sought for scalable and tunable production patchy particles, particularly those with nanoscale dimensions. In this paper synthesis via simple colloidal route is investigated. Using surfactant-free cationic polystyrene nanospheres as core gold patches produced...
It is well established that MDCK II cells grow in circular colonies densify until contact inhibition takes place. Here, we show this behavior only typical for developing on hard substrates and report a new growth phase of soft gels. At the onset, characterized by small, three-dimensional droplets attached to substrate. When area between agglomerate substrate becomes sufficiently large, very dense monolayer nucleates center colony. This monolayer, surrounded belt three-dimensionally packed...
Supported ionic liquid phase (SILP) catalysis enables a highly efficient, Ru-based, homogeneously catalyzed water-gas shift reaction (WGSR) between 100 °C and 150 °C. The active Ru-complexes have been found to exist in imidazolium chloride melts under operating conditions dynamic equilibrium, which is dominated by the [Ru(CO)3 Cl3 ]- complex. Herein we present state-of-the-art theoretical calculations elucidate mechanism more detail. We show that includes intermediate formation degradation...