The main goal of our research was to examine (1S,4R,5R)-4-(4-phenyl-1H-1,2,3-triazol-1-yl)-2-((S)-1-phenylethyl)-2-azabicyclo[3.2.1]octane (L) and its complex-forming abilities with platinum(II) ions. Herein, we present three new square planar complexes the general formulas trans-[PtCl2L2] (1), cis-[PtCl2(DMSO)(L)] (2) [Pt(DMSO)(L)(mal)] (3), where DMSO: dimethyl sulfoxide; mal: malonate. Based on experimental spectroscopic results (1H, 13C, 15N, 195Pt NMR, IR, X-ray analyses) density...

ABSTRACT The objective of this study was to evaluate complex biological properties human stem cells isolated from adipose tissue (ASCs) harvested utilizing different methods: surgical resection (R), power‐assisted liposuction (PAL), and laser‐assisted (LAL). ASCs were healthy donors, due resection, power‐, liposuction. Isolated characterized by their clonogenicity, proliferation rate, doubling time, multilineage differentiation, senescence potential. average number 1g/1 ml solid...

Hydrogels as biomaterials possess appropriate physicochemical and mechanical properties that enable the formation of a three-dimensional, stable structure used in tissue engineering 3D printing. The integrity hydrogel composition is due to presence covalent or noncovalent cross-linking bonds. Using various methods agents crucial for adjusting specific biomedical applications, e.g., direct bioprinting. research subject was mixtures gel-forming polymers: sodium alginate gelatin. polymers were...

The growing interest of oncologists in natural compounds such as polyphenols and flavonoids is encouraging the development innovative efficient carriers for delivery those drugs. This study examines carboxymethyl chitosan-based microcapsules created by spray drying a method delivering biologically active isolated from Cistus herb. Effects sterilization encapsulation on polyphenol flavonoid content extract were investigated to optimize production process. Furthermore, vitro studies carried...

This paper explores the therapeutic perspectives of polyphenols and chitosan as potential anticancer agents in mouthwash formulations. Taking into account high incidence squamous cell carcinoma (SCC) among oral cancers, this discussion will concentrate on advantages these compounds care, focusing their impact improving health cancer prevention. According to data, it appears that mixture BACs extract may increase efficiency apoptosis cells while reducing undesired side effects. The...

Because consumers are nowadays focused on their health and appearance, natural ingredients novel delivery systems one of the most developing fields pharmacy, medicine, cosmetics. The main goal this study was to design, prepare, characterize composite materials obtained by incorporation microspheres into porous polymer consisting collagen, gelatin, hydroxyethyl cellulose. Microspheres, based gellan gum xanthan with encapsulated Calendula officinalis flower extract, were produced two methods:...

The anticancer properties of fluoroquinolones and the high concentrations they achieve in urine may help bladder cancer therapy. This study aimed to analyze 4 as potential candidates for supportive treatment cancer.Comparative analyses were performed on cytotoxic effects norfloxacin, enrofloxacin, moxifloxacin, ofloxacin normal urothelial cell lines. In 2D culture, evaluated using MTT assay, real-time growth analysis, fluorescence light microscopy, flow cytometry, molecular analysis. 3D...

Drug modification with nanomaterials is a new trend in pharmaceutical studies and shows promising results, especially considering carbon-based solutions. Graphene its derivatives have attracted much research interest for their potential applications biomedical areas as drug modifiers. The following work comprehensive study regarding the toxicity of ciprofloxacin (CIP) modified by graphene oxide (GO). influence on morphology, viability, cell death pathway proliferation T24 786-0 cells was...

Rhodanine-3-acetic acid derivatives are attractive compounds with versatile effects. What is very important that of this type have many biological properties. They tested, among others, as fluorescent probes for bioimaging and aldose reductase inhibitors. also antibacterial, antifungal anticancer activity. The presented work demonstrates a slight change in the five-membered heterocyclic substituent significantly affects properties under consideration. Three rhodanine-3-acetic (A-1–A-3) were...

The aim of the study was to extend potential use human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic samples were collected during amniocentesis 165 women pregnancy. proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression surface differentiate into adipose, bone cartilage hAFSCs analyzed. Obtained results revealed that mesenchymal could be derived successfully fluid, which...

Development of biotechnology, esthetic medicine and cosmetology can enable us to slow down or delay the skin aging process. Currently, much attention is aimed at treatments using substances plant origin. They have been proven exhibit antioxidant, antibacterial antifungal properties, accelerate wound healing, moisturize skin, enhance renewal processes protect against UV radiation. Biologically active plant-derived compounds, however, are often produced by plants in very small amounts. A...

Personalized medicine is an extension of traditional based on a highly individual approach to each patient. One the most important tools that allow this targeted therapy. It focuses mainly blocking cancer cell's proliferation and angiogenesis capabilities by interfacing with specific molecules are involved in growth progression tumour. Small-molecule inhibitors monoclonal antibodies main drugs currently use order affect biochemical pathways cells. However, likewise any other therapies,...

The tumour microenvironment (TME) is the broadly understood environment around tumour. Cancer-associated fibroblasts (CAFs), Mesenchymal Stromal Cells (MSCs), and molecules synthesized by them are part of it. Both CAFs MSCs can promote progression induction an immunosuppressive, pro-inflammatory environment. Through indirect direct cell-to-cell interactions they also have a pro-proliferative, pro-angiogenic, pro-invasive effect on cancer cells. They lead to aggressive formation. At this...

Stem cell-based therapies are considered one of the most promising disciplines in biomedicine. Bladder cancer patients could benefit from directed to promote healing after invasive surgeries or lessen urinary incontinence, a common side effect both itself and treatment. However, local delivery cells producing large amounts paracrine factors may alter interactions within microenvironment. For this reason, reconstructive cellular for with history carry potential risk tumor reactivation. We...

The search for safe, cheap, and repeatable diagnostic methods is a fundamental research goal. Currently, great hope placed on fluorescence imaging. However, the development of this method mainly depends efficient fluorescent probes. Designing obtaining new probes with potential applications in imaging very difficult because compounds type must meet several requirements related to their properties. Therefore, article attempted obtain study phenanthro[9,10-d]-imidazole derivatives (PK1–PK3)...

According to the American Cancer Society, roughly 54,000 new cases of oral cavity or oropharyngeal cancers have been detected in United States America 2021, and they will cause about 10,850 deaths. The main therapies for cancer management, such as surgery radio- chemotherapy, some own benefits, albeit are often destructive surrounding tissues; thus, deep investigations into non-surgical treatments cavities needed. Biologically active compounds (BACs) extracted from European Spruce needles...

Biological tests performed using 3T3 fibroblasts indicated low cytotoxicities for the complexes mer-[Cr(pic)3] and trans(S,S)-[Cr(Cys)2]−, where pic = picolinate anion Cys cysteine. Oxidation of these by hydrogen peroxide was studied in NaOH NaHCO3 media. Electronic (UV–Vis) EPR spectroscopies were used to monitor reaction course. Hydrogen oxidizes chromium(III) both [CrV(O2)4]3− CrVIO4 2− anions alkaline media practically completely CrO4 bicarbonate solution. The reactions follow...

Background: Cardiovascular diseases are the serious clinical problem, especially loss of viable myocytes. A new approach, which provides a novel method for treatment, is tissue engineering and regenerative medicine. One current cell types used as source to improve cardiac repair, MSCs. The aim this study was check if 5’azacitidine growth factors from cardiomyocyte line initiate differentiation MSCs toward cardiomyocytes. Material methods: Bone marrow were isolated their biological features...

Background: Cartilage is an avascular tissue that has limited regenerative capacity. Stem cells, especially mesenchymal stem cells (MSCs), have been regarded as a promising cell source for cartilage repair due to their multi-lineage differentiation potential. Induction of into functional chondrocytes may resolve the above problems in repair. However, imperative develop effective strategies chondrogenic differentiation. The aim this study evaluate if induction adipose-derived (ADSCs) toward...