A5037906367- Tissue Engineering and Regenerative Medicine
- Urological Disorders and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Bladder and Urothelial Cancer Treatments
- Mesenchymal stem cell research
- Urinary Tract Infections Management
- Ureteral procedures and complications
- Microfluidic and Bio-sensing Technologies
- Telomeres, Telomerase, and Senescence
- Helicobacter pylori-related gastroenterology studies
- Pelvic floor disorders treatments
- Xenotransplantation and immune response
- Immunotherapy and Immune Responses
- Eosinophilic Esophagitis
- Cancer Cells and Metastasis
- Galectins and Cancer Biology
- Hernia repair and management
- Urinary Bladder and Prostate Research
- Cancer therapeutics and mechanisms
Nicolaus Copernicus University
2015-2023
Collegium Medicum in Bydgoszcz
2017-2023
The tissue engineering of urinary bladder advances rapidly reflecting clinical need for a new kind therapeutic solution patients requiring replacement. Majority the augmentation studies have been performed in small rodent or rabbit models. Insufficient number examining regenerative capacity tissue-engineered graft large animal model does not allow successful translation this technology to setting. aim study was evaluate role adipose-derived stem cells (ADSCs) regeneration clinically...
Abstract The use of an ileal segment is a standard method for urinary diversion after radical cystectomy. Unfortunately, utilization this can lead to numerous surgical and metabolic complications. This study aimed assess the tissue-engineered artificial conduit in porcine model. Tissue-engineered tubular polypropylene mesh scaffolds were used right ureter incontinent urostomy Eighteen male pigs divided into three equal groups: Group 1 (control ureterocutaneostomy), 2 (the ureter-artificial...
Abstract Tissue engineering allows to combine biomaterials and seeded cells experimentally replace urinary bladder wall. The normal wall however, includes branched neuronal network propagating signals which regulate urine storage voiding. In this study we introduced a novel biocomposite built from amniotic membrane (Am) graphene created interface between external stimuli replacing network. Graphene layers were transferred without modifying Am surface. Applied method allowed preserve the...
The anticancer properties of fluoroquinolones and the high concentrations they achieve in urine may help bladder cancer therapy. This study aimed to analyze 4 as potential candidates for supportive treatment cancer.Comparative analyses were performed on cytotoxic effects norfloxacin, enrofloxacin, moxifloxacin, ofloxacin normal urothelial cell lines. In 2D culture, evaluated using MTT assay, real-time growth analysis, fluorescence light microscopy, flow cytometry, molecular analysis. 3D...
Molecular mechanisms underlying the regenerative process induced by stem cells in tissue-engineered urinary bladder are poorly explained. The study was performed to explore pathways associated with regeneration reconstructed adipose tissue-derived mesenchymal stromal (ASCs).Rat bladders were acellular matrix (BAM) (n = 52) or BAM seeded (ASCs) 52). of healing analyzed at 7, 30, 90, and 180 days postoperatively using macroscopic histologic molecular techniques. Gene expression microarrays...
Many experimental approaches have been conducted in order to isolate urothelial cells from bladder tissue biopsies, but each method described has utilized different protocols and sources of tissue. In this study, we compared the methods cell isolation available literature together with standardized obtain more unified results. Five for primary porcine culture establishment were compared: explants four enzymatic utilizing collagenase II, dispase combination II trypsin, trypsin alone. The...
A key requirements for therapy utilizing the tissue engineering methodologies is use of techniques which have capability to yield a high number cells, from small biopsy in relatively short time. Up date there was no optimal methods isolation and expansion urinary bladder smooth muscle cells (UB-SMCs). The aim this study compare UB-SMCs select most repeatable efficient one.Five protocols porcine UB- SMCs including enzymatic explant three were compared. Isolation effectiveness evaluated using...
Long-term culture of mesenchymal stromal/stem cells in vitro leads to their senescence. It is very important define the maximal passage which maintain regenerative properties and can be used for cellular therapies construction neo-organs clinical application. Adipose-derived were isolated from porcine adipose tissue. Immunophenotype, population doubling time, viability using bromodeoxyuridine assay, MTT clonogencity, β-galactosidase activity, specific senescence-associated gene expression,...
Current strategies in urinary bladder augmentation include use of gastrointestinal segments, however, the technique is associated with inevitable complications. An acellular biologic scaffold seems to be a promising option for augmentation. The aim this study was evaluate utility matrix (BAM) reconstruction clinically significant large wall defects long-term porcine model. Urinary bladders were harvested from 10 pig donors. Biological scaffolds prepared by chemically removing all cellular...
Tissue engineering is focusing research effort on search for new biomaterials that might be applied to create artificial urinary conduit. Nevertheless, the demanding biomechanical characteristics necessary proper conduit function difficult replicated. In this study, we are introducing novel marine biomaterial obtained by decellularization of squid mantle derived from Loligo vulgaris. Squid mantles underwent according developed dynamic flow two-staged procedure. Efficacy method was confirmed...
Melanoma is the most dangerous type of skin cancer. Cancer stem cells (CSCs) are suspected to be responsible for cancer recurrence and in consequence therapy failure. CD133 a potential marker detection melanoma CSCs. Experiments were performed on B16-F10 mouse cell line. CD133+ isolated using an immunomagnetic sorting technique. After isolation proliferative clonogenic CD133+, CD133- CD133+/- evaluated. The tumor induction was conducted C57BL/6J model. Three different quantities (100, 1000,...
Abstract Urinary tract regeneration using tissue engineering is one of the most challenging issues in field reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after implantation. The outcome urinary bladder depended ability these cells survive, proliferate, and regenerate. aim this study was compare a sensitivity three different cell lines vitro. Three were isolated from porcine (urothelial cells, UCs smooth muscle SMCs) adipose (adipose‐derived stem ADSCs)....
H. pylori gastritis is strongly associated with the upregulation of expression several matrix metalloproteinases (MMPs) in gastric mucosa. However, role MMP-2 and MMP-9, their inhibitors (tissue -TIMPs) produced by immune cells infected children have not been clearly defined. Moreover, effects eradication therapy on MMPs TIMPs production has evaluated. A total 84 were studied: 24-with newly diagnosed gastritis, 25-after (17 them after successful therapy), pylori-negative 11-controls. Plasma...
Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, self-renewing potential porcine urothelial cells compare expression selected adhesion tight junction molecules, stem markers for clone types. were 10 bladders. Three different types: holoclone-, meroclone-and paraclone-like colonies identified based on their morphology. To characterize clones...