Identification of risk factors is important for the design control programmes mastitis in cows. Information about farms and management was collected at a farm visit. California Mastitis Test (CMT) performed to assess sub-clinical mastitis, cows, udder milk were examined clinical mastitis. A total 347 lactating cows from 83 dry season (November - February) 388 89 wet (June October) studied. The overall prevalence 19.9% 44.8% seasons, respectively. mild 17.3% 40.7%, whereas that moderate 2.6%...

The productivity as well earnings from a lamb farm largely depends on the pre-weaning survivability of lamb. This study was intended to assess factors affecting in field conditions. Data total 133 lambs produced 82 mother ewes collected beneficiary farmers three North-western districts (Gaibandha, Bogura, and Rajshahi) Bangladesh. Various non-genetic i.e. dam age, suckling, mothering behavior, sex, birth weight (g), body condition score (BCS), types were determined form weaning. results...

Sheep play a vital role in alleviating poverty and providing protein for the people of Bangladesh they are popular their mutton production. This study aimed to explore polymorphisms BMP15 gene among various Bangladeshi ram breeds devising genetic improvement strategies boost output help reduce poverty. We collected blood samples from 25 rams, including Indigenous, Mozaffarnagri, Dorper breeds, all raised traditional farming system. After extracting DNA, we amplified at Exon-1 Exon-2 using...

A study was conducted to establish a sustainable and effective manual freezing technique for cryopreservation of Bangladeshi ram semen. Three diluents techniques were tested, both as treatment combinations (diluent × technique) fixed effects or on post-thaw sperm motility (SM), viability (SV), plasma membrane integrity (SPMI) acrosome (SAI). Ten rams selected, based semen evaluation. Eight ejaculates used each combination. Semen samples diluted using two-step protocol home-made Tris-based...

Glutathione (GSH) at concentrations of 0.0 (control), 0.5, 1.0, 2.0 and 3.0 mM was added to chilled bull semen determine its effects on the keeping quality used for artificial insemination (AI). The preserved with egg yolk citrate extender. All samples were stored 4-8 degrees C 5 days. Sperm motility proportion abnormal acrosome assessed daily. significantly (p < 0.01) higher in treated 0.5 glutathione than untreated each day. optimum sperm (>or=50%) AI retained 3 days 0.0, 1.0 semen,...

The relationship between the scrotal circumference and semen volume, sperm concentration number of per ejaculate 12 crossbred bulls [4 Local × Friesian, 4 Sahiwal] was studied. Semen three consecutive collections at seven day intervals evaluated. age first collection varied 15 20 months. Soon after collection, concentration, spermatozoa initial motility fresh were measured. Scrotal correlated with in both groups. A significant (P&lt;0.04) positive correlation (r = 0.72) observed volume...

The objectives of the study were to determine prevalence repeat breeding and its associated risk factors in cows at selected areas Bangladesh followed by determination an effective treatment regime for those cows. Data collected interviewing owner from 172 farms having 1524 breedable located one Upazila Mymensingh, Chittagong, Khulna, Satkhira, Bogra Dhaka districts. A total 122 treated randomly distributed into 5 experimental groups different regimes. regimes (i) single AI with injection...

The aim of this study was to standardize the feeding regimen and body condition score (BCS) for maximum superovulatory responses in indigenous zebu cows. Ten regularly cycling 5–8‐year‐old dry cows, weighing 176–260 kg with BCS 2.5–4.5 were divided into two equal groups at random. maintained on either a good‐nutrition or high‐nutrition diet. feedstuffs analysed by proximate feed analysis metabolizable energy content estimated. After 3 months feeding, individual cows injected (i.m) 1500 IU...

Contents To determine the number of ejaculates which can be collected within a 20‐min period after smallest days sexual rest, and good diluent to preserve semen for routine AI, five mature Black Bengal bucks were used in three experiments. In experiment 1, from by using artificial vagina at homosexual mounts as many times possible during 20 min. The ejaculate numbers 3 4 (or 5 when buck could produce it) examined important characteristics. mean volume, density, mass activity, sperm motility,...

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards production of embryos.In the present study, these parameters were investigated ovaries prepubertal (n = 31) pubertal 61) black Bengal goats obtained from a slaughterhouse.Nuclear was evaluated upon aspiration following IVM TCM-199 (Earle's salt with L-glutamine sodium bicarbonate) for 27 h at 39 o C under 5% CO2 humidified air.The oocytes retrieval (mean ± SD) per 5.2 0.6 6.8 0.6,...

The study was designed to determine the effects of breed, age, parity, feeding practice, Body Condition Score (BCS), timing insemination, postpartum insemination interval and milk yield on first service pregnancy rates in cows (n = 308).The demographic factors were recorded by interviewing farmers.All examined for diagnosis rectal palpation genital tract at 60-80 days post Artificial Insemination (AI).The rate different groups compared analyzed Z test using SPSS software version 17.The...

The objective of this study was to determine if the transfection human prourokinase (ProU) gene and passage number transfected ear fibroblasts affected in vitro development bovine transgenic nuclear transfer (NT) embryos. An expression plasmid for ProU constructed by inserting a beta-casein promoter, green fluorescent protein (GFP) marker into pcDNA3 using lipid mediated method. Abattoir derived oocytes were enucleated at 18-20 hr post maturation single donor cell transferred perivitelline...

The present study was conducted to establish an efficient production system for bovine transgenic somatic cell nuclear transfer (SCNT) embryos, the effect of various conditions donor cells including type, size, and passage number on developmental competence SCNT embryos were examined with their expression rates a marker gene. An plasmid human prourokinase constructed by inserting beta-casein promoter, green fluorescent protein (GFP) gene, target gene into pcDNA3 plasmid. Three types two...

The aim of the present study was to improve cytoplasmic maturation porcine oocytes by addition lycopene into in vitro (IVM) media. We designed six experimental groups; IVM medium supplemented with 10 IU/ml FSH, FSH and human chorionic gonadotrophin (hCG), or 7 μm first half culture (0–22 h) followed further (22–44 without hCG. media delayed interruption communication between an oocyte cumulus cells. Although meiotic competence similar among groups, glutathione level matured significantly...

Ensuring a promising conception rate of non-descript zebu cows maybe an appropriate approach for cattle development and rural livelihood enhancement in Bangladesh.The study was designed to determine the factors affecting local (n = 307) under Artificial Insemination (AI) program using frozen semen.The demographic were recorded by interviewing farmers.All examined pregnancy diagnosis rectal palpation genital tract at 60-80 days post AI.The overall 57.7% required service per 2.19.The that...

This study was designed to determine and compare the serum glucose, urea nitrogen, cholesterol, total protein (TP) level in crossbred repeat breeder (RB) normally cyclic cows find out relationship of these metabolic factors with breeding syndrome (RBS).A 592 breedable from 34 farms were examined Mymensingh Chittagong districts. Seventy identified as RB another 10 randomly selected control for this study. Blood sample each cow collected separated. The samples analyzed by auto blood...

In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic nuclear transfer (iSCNT) to produce sei whale embryos and improve their developmental capacity by investigating effect osmolarity macromolecules in culture medium on vitro capacity. Hybrid produced fetal fibroblasts enucleated porcine oocytes were cultured modified zygote medium-3 examine effects serum More than 66% cells successfully fused following electrofusion. A...

The objectives of this study were to choose an effective embryo reconstruction method and post-activation agent for in vitro production sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment iSCNT embryos was performed improve the development. In Experiment 1, fusion rate significantly higher (88.1%) reconstructed using intracytoplasmic injection (ICI) than that (48.7%) subzonal insertion (SUZI) counterpart....

Reproductive diseases of crossbred dairy cows significantly reduce productivity which is great concern producers worldwide. The objectives this study were to determine the prevalence reproductive and its associated risk factors in selected areas Bangladesh. was conducted at eight villages Ullapara Upazila under Sirajganj district. A total 197 farms having 488 breedable (395 + 93 heifers) randomly selected. questionnaire as well examination made collect data on occurrence diseases. overall...

Ultrasonography and a structured questionnaire were used in cross-sectional study to gather data on the prevalence risk factors for anestrus crossbred cows at =>60 days postpartum 273 smallholder farms. The of was 18%. odds ratio (OR) true 17.52 2.81 times higher (P < 0.05) with poor (=3.5) body condition score (BCS), respectively, compared those optimal BCS (2.5-3.5), 2.82 suckled than nonsuckled = 0.03), 2.53 that calved during cold season hot 0.03). OR 1.62 0.017) managed by an employee...