Abstract Incidence of enteric viruses in sewage, the efficacy wastewater treatment plants to remove these viruses, and health effects from their release into surface water are very important environmental issues microbiology field. One most pathogenic is adenovirus which can cause a serious disease such as gastroenteritis with low grade fever mild dehydration humans. In this study we performed qualitative polymerase chain reaction (PCR) analysis HAdV on 60 stool samples children acute...

<b>Background and objective</b> Medicinal mushrooms, as well lichens are potential sources of bioactive compounds against many lethal diseases. Therefore, in-vitro evaluations the biological activities 80% methanolic extracts two Japanese originated samples, namely, mushroom <i>Ganoderma applanatum</i>, lichen <i>Candelariella vitellina</i> have been performed.<br><b>Materials methods</b> Extraction <i>C. <i>G. applanatum</i> was conducted using methanol. The resulted were examined for their...

Abstract HAdV and HBoV are frequently found in different water sources throughout the year. Due to their health impact on humans, continuous monitoring of these viruses is considered an important tool evaluate circulation within society. This study investigates prevalence environmental samples collected from western Nile Delta Egypt. In total, 264 were treated untreated sewage (48), sludge (24), drainage (96), sediment (96) December 2019 November 2021. Molecular detection was performed...

The bacterial profiles of natural household biofilm have not been widely investigated. majorities these lineages are cultivable. Thus, this study aims (i) to enumerate some potential using culture based method within samples and confirmed Biolog GEN III polymerase chain reaction (PCR). (ii) To investigate the communities in two next generation sequencing (NGS). Forty were cultured colonies each selected prevailing (E. coli, Salmonella entrica, Pseudomonas aeruginosa, Staphylococcus aureus...

Abstract Wastewater plays a major role in water pollution causing transmission of several viral pathogens, including Aichi virus (AiV) and human bocavirus (HBoV), associated with gastrointestinal illness humans. In this study, we investigated the presence AiV HBoV aquatic, sludge, sediment matrices collected from Abu-Rawash wastewater treatment plant (WWTP), El-Rahawy drain, Rosetta branch River Nile Egypt by conventional polymerase chain reaction (PCR). RNA was detected 16.6% (2/12), 8.3%...

This paper aims to quantify human adenovirus (HAdV), rotavirus species A (RVA), and hepatitis virus (HAV) in surface water sediments determine the viability of HAdV these samples. Water sediment samples were collected, HAdV, RVA, HAV quantified by real-time polymerase chain reaction (PCR); was also evaluated for infectivity a plaque assay (PA). For samples, detected 70.8% summer collections, with 82.4% containing infectious HAdV; incidence winter 62.5%. 37.5% 66.7% 37.5%. RVA 20.8 45.8%...

A chalcone bearing naphthalene–benzofuran rings was synthesized. The behavior of this towards some nitrogen and carbon nucleophiles with formation pyrazole, isooxazole, thiazole, pyrimidine, pyran, pyridine studied. Also, acetohydrazide synthesized utilized as a key starting material in the synthesis 2,4‐dioxothiazolidine 2,4‐dioxoquinazolin derivatives. structures all products obtained were illustrated from their analytical spectral data. Some new compounds selected evaluated antiviral...

Abstract Sewage discharge is considered to be the primary source of viral contamination in aquatic environments. This study was conducted evaluate impact El-Rahawy wastewater on water quality Rosetta branch River Nile (Rosetta Nile) through detection astrovirus (AstV) and norovirus (NoV) sediments both sites. For this purpose, we collected 72 12 sediment samples from drain, river before after mixing with between April 2017 March 2018. AstVs NoVs were identified (40.2% versus 25%), (41.6%...

AbstractFor the first time in this study, silver nanoparticles (AgNPs) and gold (AuNPs) were green synthesized by cost-effective eco-friendly procedure using Cotton seed meal Fodder yeast extracts. The biosynthesized NPs characterized UV–Vis spectroscopy, dynamic light scattering analysis (DLS), transmission electron microscopy (TEM), selected area diffraction (SAED), fourier-transform infrared (FTIR) spectroscopy. Furthermore, tested vitro against biofilm formation some pathogenic negative...

In this study, the prevalence of astrovirus (AstV), norovirus (NoV), and group C rotaviruses (RVC) were described in urban sewage, sewage sludge, river water, sediment samples from Egypt. AstV, NoV, RVC detected 58.3%, 33.3%, 25% raw samples; 25%, 16.6% treated 66.6%, 16.6%, 8.3% sludge samples, respectively. On other hand, water 8.3%, 0% The study revealed that AstV genogroup B NoV I are most frequent genotypes Egyptian environment. Furthermore, peak was found winter months however there no...

Rotavirus group A (RV-A) and Cryptosporidium spp.are recognized as important pathogens causing gastroenteritis, even with very low doses in all age groups representing a public health risk.Seventy-two wastewater samples were collected concentrated for detecting spp.and RV-A.PCR was performed identification of genus using generic primers species specific parvum / hominis.Multiplex seminested RT-PCR to identify the genotypes P G RV-A.Our findings showed that rotavirus genome detected 29...

Background and objective Medicinal mushrooms are mines of various biologically active compounds. Therefore, chemical analysis in-vitro evaluation some biological activities the Japanese originated mushroom Ganoderma spp. were conducted. Materials methods Extraction fruiting bodies was accomplished using 80% methanol. This extract investigated for its cholesterol-lowering activity, anti-rotavirus effect, anti-human colon cancer influence. Moreover, a gas chromatography–mass spectrometry this...

Group C rotavirus (RVC) has been identified as important enteric pathogen associated with acute gastroenteritis in human, especially outbreaks. To evaluate the presence of RVC environmental samples, one-year study was performed Giza, Egypt. A total 64 sewage (raw and treated) 32 sludge samples from Zenin wastewater treatment plant (WWTP) 72 drainage water 24 sediment El-Rahawy drain which receives effluent WWTP were collected. After concentration collected semi-nested RT-PCR using specific...