A5006700378- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Carbon dioxide utilization in catalysis
- biodegradable polymer synthesis and properties
- Asymmetric Hydrogenation and Catalysis
- Protein Structure and Dynamics
- Vanadium and Halogenation Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Catalytic C–H Functionalization Methods
- Oxidative Organic Chemistry Reactions
- Polysaccharides and Plant Cell Walls
- Enzyme Structure and Function
Tianjin Institute of Industrial Biotechnology
2020-2025
Chinese Academy of Sciences
2020-2025
Intelligent Synthetic Biology Center
2025
State Key Laboratory of Medicinal Chemical Biology
2017
Nankai University
2017
Starches, a storage form of carbohydrates, are major source calories in the human diet and primary feedstock for bioindustry. We report chemical-biochemical hybrid pathway starch synthesis from carbon dioxide (CO2) hydrogen cell-free system. The artificial anabolic (ASAP), consisting 11 core reactions, was drafted by computational design, established through modular assembly substitution, optimized protein engineering three bottleneck-associated enzymes. In chemoenzymatic system with spatial...
Formolase catalyzes the carboligation of C1 compound formaldehyde, providing a unique opportunity for producing multicarbon compounds with formaldehyde as sole substrate. Elucidating molecular rationale determining activity formolase, especially at low concentrations is essential improving its catalytic performance in conversion. In this study, channel-modulating helix formed zipper structure neighboring that was identified to regulate formolaseBFD, formaldehyde. Up 24.1-fold increased...
Bromide-mediated intermolecular annulation of phenylethanone derivatives with alkynes has been developed, which allows for the regioselective formation polysubstituted 1-naphthols. The usage readily available bromine catalyst, broad substrate scope, and mild conditions make this protocol very practical. Mechanistic investigations reveal that bromination occurs to yield bromo-substituted intermediates, react in situ furnish desired
Enzymatic carboligation coupled with selective oxidation converts formaldehyde to glycolic acid, a monomer of biodegradable polymers.
A green chemoenzymatic reaction converts C1 compound formaldehyde into lactic acid using a newly identified formolase variant and NaOH as catalysts.
Mechanosensitive amino acid exporters have drawn increasing attention due to their important roles in extracellular accumulation of the target acids. Protein engineering is a powerful approach tailor properties and illustrate structure-function relationships. Here we report first protein effort on mechanosensitive glutamate exporter MscCG2 from Corynebacterium glutamicum for improved excretion efficiency understanding relationship. was engineered through directed evolution computer-assisted...
Here, we report an enzymatic cascade reaction converting high concentration of formaldehyde into L-threitol. The starts with the carboligation catalyzed by formolase and fructose-6-phosphate aldolase, generating...