A5103017865- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Microbial Natural Products and Biosynthesis
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Forest Insect Ecology and Management
- Mycorrhizal Fungi and Plant Interactions
- Insect and Pesticide Research
- Photosynthetic Processes and Mechanisms
- Biochemical and Structural Characterization
- Cholinesterase and Neurodegenerative Diseases
- Entomopathogenic Microorganisms in Pest Control
- Fungal Infections and Studies
- Glycosylation and Glycoproteins Research
- Insect symbiosis and bacterial influences
- Pharmacogenetics and Drug Metabolism
- Legume Nitrogen Fixing Symbiosis
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- Transgenic Plants and Applications
- Forest Ecology and Biodiversity Studies
- Synthesis and biological activity
- Plant responses to elevated CO2
- Genomics, phytochemicals, and oxidative stress
National Institute of Chemistry
2013-2024
Karolinska Institutet
2015
University of Ljubljana
2008
The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens humans, animals and crops, a source potent carcinogenic contaminants food, an genetic model. genome sequences eight aspergilli have already been explored investigate aspects biology, raising questions about evolution specialization within this genus.We generated for ten novel, highly diverse species compared these in detail sister more distant genera....
Aims CYP53A15, from the sorghum pathogen Cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate aromatic compound metabolism fungi. Because this enzyme unique to fungi, it promising drug target fungal pathogens other eukaryotes. Methods and Results In our work, we showed high antifungal activity seven cinnamic acid derivatives against C. lunatus two Aspergillus niger Pleurotus ostreatus. To elucidate mechanism action with most potent properties, studied...
Summary Cytochromes P450 (CYPs) catalyse diverse reactions and are key enzymes in fungal primary secondary metabolism, xenobiotic detoxification. CYP enzymatic properties substrate specificity determine the reaction outcome. However, CYP‐mediated may also be influenced by their redox partners. Filamentous fungi with numerous CYPs often possess multiple microsomal partners, cytochrome reductases (CPRs). In plant pathogenic ascomycete Cochliobolus lunatus we recently identified two CPR...
Cytochrome P450 monooxygenases (CYPs/P450s) are heme-thiolate proteins whose role as a drug target against pathogenic microbes has been explored because of their stereo- and regio-specific oxidation activity. We aimed to assess the CYP53 family's common alternative animal (including human) plant fungi its in fungal-mediated wood degradation. Genome-wide analysis fungal species revealed presence members ascomycetes basidiomycetes. Basidiomycetes had higher number genomes than ascomycetes....
A novel cytochrome P450, CYP53A15, was identified in the pathogenic filamentous ascomycete Cochliobolus lunatus. The protein, classified into CYP53 family, capable of para hydroxylation benzoate. Benzoate is a key intermediate metabolism aromatic compounds fungi and yet basically toxic to organism. To guide functional analyses, protein structure predicted by homology modeling. Since many naturally occurring antifungal phenolic are structurally similar CYP53A15 substrates, we tested their...
Abstract 17β‐Hydroxysteroid dehydrogenase (17β‐HSDcl) from the filamentous fungus Curvularia lunata (teleomorph Cochliobolus lunatus ) catalyzes NADP(H)‐dependent oxidoreductions of androgens and estrogens. Despite detailed biochemical structural characterization 17β‐HSDcl, its physiological function remains unknown. On basis amino acid sequence alignment, phylogenetic studies, recent identification substrates homologous MdpC Aspergillus nidulans AflM parasiticus , we propose an...
Fungal CYP53 enzymes are highly conserved proteins, involved in phenolic detoxification, and have no homologues higher eukaryotes, rendering them favorable drug targets. Aiming to discover novel inhibitors, we employed two parallel virtual screening protocols evaluated highest scoring hit compounds by analyzing the spectral binding interactions, surveying antifungal activity, assessing inhibition of catalytic activity. On basis combined results, selected 3-methyl-4-(1H-pyrrol-1-yl)benzoic...
Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This decreased significantly during the time growth (1-3 days). Moreover, when A. was carbon-deprived medium, efficiency this ethanolic much lower than carbon-enriched and became almost undetectable after 3 days medium. The lipid composition these analysed by chromatography-electrospray ionisation tandem mass spectrometry. can be mainly...
Proteins of the aegerolysin family have a high abundance in Fungi. Due to their specific binding membrane lipids, and membrane-permeabilization potential concert with protein partner(s) belonging membrane-attack-complex/perforin (MACPF) superfamily, they were proposed as useful tools different biotechnological biomedical applications. In this work, we performed functional studies on expression genes encoding MACPF-like proteins Aspergillus niger. Our results suggest sporulation process being...