A5073704129- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Listeria monocytogenes in Food Safety
- Artificial Intelligence in Healthcare
- Nutrition and Health in Aging
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Diabetes Management and Research
- Global Maternal and Child Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Health disparities and outcomes
- Insect and Pesticide Research
- Vibrio bacteria research studies
- Health, Environment, Cognitive Aging
- Global Health Care Issues
- Escherichia coli research studies
- Health and Well-being Studies
- Colorectal Cancer Screening and Detection
- Probiotics and Fermented Foods
- Mobile Health and mHealth Applications
- Nutritional Studies and Diet
- Metabolomics and Mass Spectrometry Studies
- CRISPR and Genetic Engineering
University Kebangsaan Malaysia Medical Centre
2021-2025
Virginia Tech
2024
Shanghai Jiao Tong University
2021-2024
National University of Malaysia
2023
VRprofile2 is an updated pipeline that rapidly identifies diverse mobile genetic elements in bacterial genome sequences. Compared with the previous version, three major improvements were made. First, user-friendly visualization could aid users investigating antibiotic resistance gene cassettes conjunction various multiple region mosaic structure. compare predicted to collected known similar architecture. A new mobilome indicator was proposed give overall estimation of size individual...
Abstract TADB 3.0 (https://bioinfo-mml.sjtu.edu.cn/TADB3/) is an updated database that provides comprehensive information on bacterial types I to VIII toxin–antitoxin (TA) loci. Compared with the previous version, three major improvements are introduced: First, aid of text mining and manual curation, it records details 536 TA loci experimental support, including 102, 403, 8, 14, 1, 3 4 VIII, respectively; Second, by leveraging upgraded prediction tool TAfinder 2.0 a stringent strategy,...
ABSTRACT Background Emerging evidence suggests that the creatinine‐to‐body weight (Cre/BW) ratio is a predictor for incident diabetes in Asian population. This study examined association between Cre/BW and diabetes, as well relationship skeletal muscle body fat mass multiethnic Malaysian cohort. Methods A total of 13 047 eligible participants were selected from 119 560 The Cohort participants. Of these, 750 who developed cases, while 3750 controls chosen randomly healthy nested case–control...
Understanding how bacterial pangenomes originate and evolve is crucial for predicting evolutionary trajectories uncovering ecological interactions. Bacterial pangenome fluidity has been attributed to adaptive evolution, yet its specific drivers remain be elucidated. Listeria monocytogenes (Lm), a foodborne pathogen causing fatal listeriosis, serves as an ideal model studying the mechanisms underlying due high divergence, versatility, public health relevance. Through analysis of 177 Lm...
Soil is an important reservoir of antibiotic resistance genes (ARGs) and understanding how corresponding environmental changes influence their emergence, evolution, spread crucial. The soil-dwelling bacterial genus Listeria, including L. monocytogenes, the causative agent listeriosis, serves as a key model for establishing this understanding. Here, we characterize ARGs in 594 genomes representing 19 Listeria species that previously isolated from soils natural environments across United...
There is an increasing prevalence of chronic kidney disease (CKD) in Malaysia, hence identifying factors associated with the early stage CKD crucial for preventive measures. This study investigated association between various and their interaction a multi-ethnic Malaysian cohort. A nested case-control analysis was conducted on 3160 eligible participants renal profile data from The Cohort project. status determined by estimated glomerular filtration rate (eGFR) using Chronic Kidney Disease...
Background: Colorectal cancer (CRC) is one of the most common cancers globally, with immunochemical faecal occult blood test (iFOBT) frequently used for population level screening. This study evaluated CRC screening uptake among urban-poor individuals aged 40–65, assessed their knowledge risk factors and symptoms before after an educational programme, identified determinants polyps within this group. Methods: A cross-sectional recruited 577 from seven People’s Residential Project (PPR) areas...
Hypervirulent
Abstract Soil has been identified as an important reservoir of antibiotic resistance genes (ARGs) and there is a need to understand how corresponding environmental changes influence their emergence, evolution, spread. As soil-dwelling bacterial genus containing pathogens, Listeria, including L. monocytogenes , the causative agent listeriosis in humans, could serve key model for establishing this understanding. Notably, acquired among isolated from foods environment observed some regions over...
The attrition rate of longitudinal study participation remains a challenge. To date, the Malaysian Cohort (TMC) follow-up was only 42.7%. This objective is to identify cause among TMC participants and measures curb it. A total 19,343 from Kuala Lumpur Selangor that due for were studied. two most common reasons are undergoing medical treatment at another government or private health center (7.0%) loss interest in participating project (5.1%). Those who inclined drop out mostly Chinese, aged...
Glycated haemoglobin (HbA1c) is a standard indication for screening type 2 diabetes that also has been widely used in large-scale epidemiological studies. However, its long-term quality (in terms of reproducibility) stored liquid nitrogen still unknown. This study aimed to evaluate the stability and reproducibility HbA1c measurements from frozen whole blood samples kept at -196°C more than 7 years.A total 401 with fresh measurement were randomly selected The Malaysian Cohort's (TMC) biobank....
Abstract Background Bacterial toxin-antitoxin (TA) modules respond to various stressful conditions. The Gcn5-related N-acetyltransferase-type toxin (GNAT) protein encoded by the GNAT-RHH TA locus is involved in antibiotic tolerance of Klebsiella pneumoniae. Objectives To investigate transcriptional mechanism operon kacAT under stress. Methods level K. pneumoniae was measured quantitative real-time (qRT) PCR assay. degradation antitoxin KacA examined western blot and fluorescent protein....
The management of extensive longitudinal data in cohort studies presents significant challenges, particularly middle-income countries like Malaysia where technological resources may be limited. These challenges include ensuring integrity, security, and scalability storage solutions over extended periods.
Toxin-antitoxin (TA) modules containing a Gcn5-related N-acetyltransferase (GNAT) toxin domain regulate bacterial physiology under adverse environmental stresses. Multiple GNAT-ribbon-helix-helix (RHH) TA loci have been identified in single genomes. However, their diversity and interactions are still obscure. Our previous analysis showed that the GNAT of Klebsiella pneumoniae, KacT, introduces antibiotic tolerance toxicity is neutralized by KacA, an RHH antitoxin. We here present...