A5027311410- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Tuberculosis Research and Epidemiology
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Microbial Natural Products and Biosynthesis
- 3D Printing in Biomedical Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Plant tissue culture and regeneration
- Pneumonia and Respiratory Infections
- Toxin Mechanisms and Immunotoxins
- Cytomegalovirus and herpesvirus research
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Cancer Research and Treatments
Trinity College Dublin
2023
Texas A&M University System
2002-2010
Ollscoil na Gaillimhe – University of Galway
2006-2008
University of Oxford
2005-2006
Mesenchymal stem cells (MSCs) have been proposed for use in combinatorial gene and cell therapy protocols the treatment of disease promotion repair. The efficacy such a therapeutic approach depends on determination which vectors give maximal transgene expression with minimal death. study was carried out bone-marrow derived rat MSCs, range tested same preparation. Adenovirus, adeno-associated virus (AAV; serotypes 1, 2, 4, 5, 6), lentivirus, nonviral were compared. Lentivirus proved to be...
Intravenous delivery of adenovirus vectors requires that the virus is not inactivated in bloodstream. Serum neutralizing activity well documented, but we show here type 5 also interacts with human blood cells. Over 90% a typical dose binds to (but murine) erythrocytes ex vivo, and samples from patient administered clinical trial showed over 98% viral DNA was cell associated. In contrast, nearly all genomes murine bloodstream are free plasma. Adenovirus bound cells fails infect A549 lung...
Abstract Lung cancer is a major public health problem in the western world, and gene therapy strategies to tackle this disease systemically are often impaired by inefficient delivery of vector tumour tissue. Some main factors inhibiting systemic found blood stream form red white cells (WBCs) serum components. Mesenchymal stem (MSCs) have been shown home sites could potentially act as shield vehicle for tumouricidal vector. Here, we describe ability an adenoviral expressing TRAIL (Ad.TR)...
Growth and a number of differentiated characteristics cultured epidermal cells from the rainbow trout Oncorhynchus mykiss were compared using two commercially available serum–free media, dermal substrate/serum free kit serum–containing medium which had been previously optimized for cell culture. Each supported short term growth over 15 days. Only supplied substrate culture longer periods. This was use with collagen/stromal but gave good cultures even without substrate. Differentiation,...
Alveolar macrophages are likely the first cell type to encounter Mycobacterium tuberculosis in a pulmonary infection, resulting production of chemokines. In order evaluate this response, alveolar harvested from nonvaccinated and bovis BCG-vaccinated guinea pigs were infected vitro with live M. H37Ra or H37Rv (multiplicity 1:1) cultured lipopolysaccharide (10 micro g/ml) for 3, 12, 24 h. Interleukin-8 (IL-8) monocyte chemoattractant protein 1 (MCP-1) mRNA expression was determined by...