Lucía Martínez-Fresneda

ORCID: 0000-0002-0967-8593
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About
Contact & Profiles
Research Areas
  • Sperm and Testicular Function
  • Reproductive Biology and Fertility
  • Reproductive biology and impacts on aquatic species
  • Reproductive Physiology in Livestock
  • Effects of Environmental Stressors on Livestock
  • Genetic and phenotypic traits in livestock
  • Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
  • Plant Reproductive Biology
  • Animal Genetics and Reproduction
  • Animal Virus Infections Studies
  • Agriculture and Biological Studies

Institute of Agrifood Research and Technology
2017-2021

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2017-2021

University of Bonn
2018-2021

Centro Regional de Selección y Reproducción Animal
2021

Universidad de Murcia
2018-2021

University of Limerick
2016-2018

The aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with head area seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout year, which could interfere spermatogenesis cryopreservation. Ejaculates, testicular biopsies blood were collected middle at end RS (Middle-RS vs End-RS) periods high low in Merino Mouflon rams. Fresh frozen-thawed quality, morphometry, tubule morphometry...

10.1071/rd18511 article EN Reproduction Fertility and Development 2019-01-01

Abstract Spermatozoa must undergo the process of capacitation to fertilize egg which involves a cell destabilizing process. Capacitation-like changes such as protein tyrosine phosphorylation (PTP) are associated with cryopreservation. The aim this study was compare cryoresistance and response epididymal ejaculated sperm European mouflon ( Ovis musimon ). Post-thaw parameters were analysed from samples cryopreserved by slow-freezing or ultrarapid-freezing for comparison. Sperm status assessed...

10.1038/s41598-019-52057-0 article EN cc-by Scientific Reports 2019-10-30

In small ruminants, testosterone and prolactin plasma concentrations show circannual fluctuations as an adaptation mechanism to their seasonal breeding behavior. Sperm resistance the freezing–thawing process shows fluctuation throughout year, with lower sperm freezability at beginning of season when levels reach maximum concentration. Nevertheless, whether these hormones directly affect post-thaw quality parameters is still unclear. The objective was study effect or added in vitro on...

10.1016/j.domaniend.2019.06.004 article EN cc-by-nc-nd Domestic Animal Endocrinology 2019-07-05

Abstract The journey of spermatozoa through the female genital tract is facilitated by rheotaxis, or cell’s preference to swim against a flow, as well thigmotaxis, wall tracking behaviour, which guides them site fertilisation. aim this study was characterise rheotactic and thigmotactic response stallion sperm within microfluidic channel. Stallion rheotaxis assessed channel with regard to: (i) A range flow velocities, (ii) Varying media viscosity (iii) Sperm hyperactivation. distribution...

10.1038/s41598-018-34973-9 article EN cc-by Scientific Reports 2018-11-09

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied threatened related species from family Phoenicopteridae, and permit genetic material banking. Current study sought develop effective sperm protocols through examining influences two permeating cryoprotectants seminal plasma removal. During consecutive years (April), samples were collected frozen flamingos. In first year, effect cryoprotectants, DMA (dimethylacetamide)...

10.3390/ani11010203 article EN cc-by Animals 2021-01-15

Sperm cryopreservation protocols are not well defined in many wild species such as the mouflon. The aim was to study effect of different concentrations EY on mouflon epididymal sperm cryosurvival. Samples were collected by flushing method and cryopreserved conventional freezing technique straws using a TEST (TES, Tris, glucose) 5% v/v glycerol medium containing either 6% (n=16) or 12% (n=13) clarified EY. membrane integrity, acrosome motility, morphological abnormalities assessed fresh...

10.5424/sjar/2018163-13268 article EN Spanish Journal of Agricultural Research 2018-10-03

Aim: This work examined the influence of induced changes in prolactin (PRL) secretion on sperm cryoresistance ibex and mouflon. Materials Methods: PRL was modified a first experiment by use bromocriptine (BCR, dopamine agonist) during non-breeding season, second sulpiride (SLP, D2-receptor antagonist) rutting season. Slow ultra-rapid freezing protocols were used to cryopreserve samples. Results: BCR decreased blood plasma concentrations, whereas SLP increased them. Cryoresistance ratios...

10.1089/bio.2021.0129 article EN Biopreservation and Biobanking 2022-06-08

Gonadotrophin deficiency, a condition usually present from birth, can cause delayed puberty in humans and domestic animals, but this has not been confirmed wild mountain ruminants.During the rutting season (December), signs of sexual immaturity were detected 2.5 yearold male Iberian ibex (Capra pyrenaica) maintained captivity.Cryptorchidism right testicle was diagnosed, along with complete adherence urethral process to prepuce.The animal treated five injections 500 UI hCG, administered...

10.17140/vmoj-2-116 article EN Veterinary Medicine - Open Journal 2017-05-26
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