A5075603539- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Avian ecology and behavior
- Animal Nutrition and Physiology
- Insect and Arachnid Ecology and Behavior
- Wildlife Ecology and Conservation
- Plant Reproductive Biology
- Reproductive Health and Technologies
- Animal Behavior and Welfare Studies
- Bee Products Chemical Analysis
- Animal Genetics and Reproduction
- Signaling Pathways in Disease
- Ovarian function and disorders
- Livestock and Poultry Management
- Meat and Animal Product Quality
- Effects of Environmental Stressors on Livestock
Junta de Castilla y León
2023-2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2015-2022
Institute of Agrifood Research and Technology
2019
Centro Regional de Selección y Reproducción Animal
2015-2016
Instituto de Biología Molecular y Celular de Rosario
2009-2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2009-2014
National University of Rosario
2008-2014
The aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with head area seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout year, which could interfere spermatogenesis cryopreservation. Ejaculates, testicular biopsies blood were collected middle at end RS (Middle-RS vs End-RS) periods high low in Merino Mouflon rams. Fresh frozen-thawed quality, morphometry, tubule morphometry...
This work examines the effects of subsequent cycles freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, assesses whether density-gradient centrifugation (DGC) can increase number this withstand. A sample was collected by electroejaculation from a mature subjected to five cycles. Although repeated negatively affected (P < 0.05) motility membrane integrity, in both nonselected DCG-selected samples, >60% cells treatments showed acrosome integrity even after...
In small ruminants, testosterone and prolactin plasma concentrations show circannual fluctuations as an adaptation mechanism to their seasonal breeding behavior. Sperm resistance the freezing–thawing process shows fluctuation throughout year, with lower sperm freezability at beginning of season when levels reach maximum concentration. Nevertheless, whether these hormones directly affect post-thaw quality parameters is still unclear. The objective was study effect or added in vitro on...
Animals with external fertilization, as amphibians, store their sperm in a quiescent state the testis. When spermatozoa are released into natural fertilization media, hypotonic shock triggers activation of motility. Rhinella (Bufo) arenarum immotile artificial seminal plasma (ASP, resembling testicular tonicity) but acquire situ flagellar beating upon dilution. However, if components from egg shelly coat added to this medium, motility shifts progressive pattern. Recently, we have shown that...
The domestication process has resulted in profound changes the reproductive physiology of animals that might have affected sperm characteristics and thus their sensitivity to handling cryopreservation procedures. This work assesses response domestic wild ungulates a cooling storage at 15°C for 20 h followed by incubation 38.5°C, 5% CO 2 , h. In addition, this paper examines most representative traits assess responsiveness these stress conditions. Sperm samples were collected from ancestor...
Sperm from the toad Bufo arenarum must penetrate egg jelly before reaching vitelline envelope (VE), where acrosome reaction is triggered. When coat removed, sperm still bind to VE, but acrosomal exocytosis not promoted. Our previous work demonstrated that diffusible substances of coat, termed "egg water" (EW), triggered capacitation-like changes in B. sperm, promoting acquisition a transient fertilizing capacity. In present work, we correlated this capacity with ability undergo reaction,...
Abstract Background The Cantabrian capercaillie ( Tetrao urogallus cantabricus ) is critically endangered. This subspecies has the lowest genetic variability and it in regression. It belongs to Phasianidae family; therefore, domestic chicken Gallus gallus domesticus could be a good model for developing reproductive technologies use populations with low availability of animals. Objectives In this study, we analyzed response sperm freezing–thawing process contributing development semen...
This work examines the effect of equilibration time with extender on ultra-rapidly frozen-thawed wild ruminant epididymal (origin: Iberian ibex) and ejaculated mouflon) sperm variables. Sperm samples were prepared either without prior equilibration, or equilibrated for 30 min before freezing. Higher quality (p < 0.05) spermatozoa obtained when was allowed, in terms motility, acrosome apical ridge integrity, viability, percentage normal cells, linearity straightness movement. The head area,...
Animal capture is essential in conservation and management programs, both for captive wild species. This paper describes the effect of midazolam sedation domestic phasianids with respect to dorso-abdominal massage obtaining semen samples, on quality sperm thus collected. Three experiments were performed. Experiment 1 - Determination, quality, optimum treatment chicken. involved sedating birds either: 1) 2 mL saline (control [C]), 2) mg/kg (low dose [LD]), 3) 6 (high [HD]), or 4) waiting 10...
The use of condoms could provide a means collecting high-quality spermatozoa from different species under physiological ejaculation conditions. However, certain condom materials may affect sperm functionality. This study examined the spermiotoxicity commercial towards ram and goat spermatozoa. Sperm samples were diluted in Tyrode's medium placed contact with piece material (polyurethane, polyisoprene or latex) incubated for 30 90 min. Contact time latex treatments affected some variables; no...
With the threat of extinction looming over many species, development assisted reproduction techniques for use in conservation programmes is imperative. This work explores feasibility and efficacy artificial insemination capercaillie (Tetrao urogallus), a species critical danger extinction. Nine young, male birds were used as sperm donors five young females. Three females laid 19 viable eggs, which 13 fertilized (68%). research contributes to scientific understanding capercaillie's...