A5102913327- Sperm and Testicular Function
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Reproductive Biology and Fertility
- Proteins in Food Systems
- Aquaculture disease management and microbiota
- Fish biology, ecology, and behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Biology and Ecology Studies
- Growth Hormone and Insulin-like Growth Factors
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Protein Interaction Studies and Fluorescence Analysis
- Identification and Quantification in Food
- Birth, Development, and Health
- Zebrafish Biomedical Research Applications
- Parasite Biology and Host Interactions
- Ichthyology and Marine Biology
- RNA Interference and Gene Delivery
- DNA and Biological Computing
- Muscle Physiology and Disorders
- Research on Leishmaniasis Studies
- Agricultural and Food Production Studies
- RNA Research and Splicing
National University of Rosario
2010-2022
Centro Científico Tecnológico Mar del Plata
2021
Instituto de Biología Molecular y Celular de Rosario
2008-2018
Consejo Nacional de Investigaciones Científicas y Técnicas
2000-2016
Universidade de Santiago de Compostela
2012
Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I valid of 79 freshwater from the Lower Paraná River. Neighbour-joining analysis K2P genetic distances formed non-overlapping clusters almost all with ≥99% bootstrap support each. Identification was successful 97.8% as minimum distance to nearest neighbour exceeded maximum intraspecific in...
Abstract This article documents the addition of 268 microsatellite marker loci to M olecular E cology R esources D atabase. Loci were developed for following species: A lburnoides bipunctatus, C hamaerops humilis, hlidonias hybrida, yperus papyrus, F usarium graminearum, L oxigilla barbadensis, acrobrachium rosenbergii, O dontesthes bonariensis, P elteobagrus vachelli, osidonia oceanica, otamotrygon motoro, hamdia quelen, S arotherodon melanotheron heudelotii, ibiraea angustata, T akifugu...
Plasma membrane hyperpolarization is crucial for mammalian sperm to acquire acrosomal responsiveness during capacitation. Among the signaling events leading capacitation, immediate activation of protein kinase A plays a pivotal role, promoting subsequent stimulation tyrosine phosphorylation that associates with fertilizing capacity. We have shown previously mice deficient in cSrc are infertile and exhibit improper cauda epididymis development. It therefore not clear whether lack...
The present study evaluates the influence of continuous light on phenotypic sex ratios in Chirostoma estor , a temperature determination animal model. Relative gene expression levels 5 day old larvae were performed two early gonad differentiation genes ( sox9 and foxl2 ), stress axis activation gcr1 crf ) four reactive oxygen species (ROS) antagonist effector sod2, ucp2, gsr cat ). Two treatments applied from fertilization; control (12L:12D) simulated natural photoperiod illumination...
Abstract Zebrafish ( Danio rerio ) is increasingly employed for evaluating toxicity and drug discovery assays. Commonly experimental approaches biotoxicity assessment are based on visual inspection or video recording. However, these techniques limited large‐scale assays, as they demand either a time‐consuming detailed of the animals intensive computing resources in order to analyze considerable amount screenshots. Recently, we have developed simple methodology tracking locomotor activity...
Currently, the storage of fish spermatozoa through cryopreservation is widely used.Although it common knowledge that process freezing / thawing generates DNA fragmentation spermatozoa, consequences this on embryonary development are unknown.There great interest in developing methods for vitro fertilization species Prochilodus lineatus using cryopreserved semen.In paper we study embryonic fish, to lay groundwork observation abnormalities embryos derived from criopreserved spermatozoa.The eggs...
Abstract Nucleic-acid barcoding is an enabling technique for many applications, but its use remains limited in emerging long-read sequencing technologies with intrinsically low raw accuracy. Here, we apply so-called NS-watermark barcodes, whose error correction capability was previously validated silico, a proof of concept where synthesize 3840 barcodes and them to asymmetrically tag simultaneously sequence amplicons from two evolutionarily distant species (namely Bordetella pertussis...
Twenty polymorphic microsatellite markers were developed for the Neotropical fish Leporinus obtusidens using a next generation sequencing approach and tested in two other characifomes species, Schizodon platae Prochilodus lineatus . Microsatellite loci alleles L. ranged between 2 20 per locus (mean = 5·7), with expected heterozygosity values ranging from 0·097 to 0·956 0·578) observed 0·000 0·800 0·400) sample of specimens lower Paraná River (Argentina). Most these will be valuable tool...
Animals with external fertilization, as amphibians, store their sperm in a quiescent state the testis. When spermatozoa are released into natural fertilization media, hypotonic shock triggers activation of motility. Rhinella (Bufo) arenarum immotile artificial seminal plasma (ASP, resembling testicular tonicity) but acquire situ flagellar beating upon dilution. However, if components from egg shelly coat added to this medium, motility shifts progressive pattern. Recently, we have shown that...
Different strains of Nile tilapia can be found worldwide. To successfully use them in breeding programs, they must genetically characterized. In this study, four - UFLA, GIFT, Chitralada and Red-Stirling were characterized using 10 noncoding microsatellite loci two microsatellites located the promoter first intron growth hormone gene (GH). The GH identified at positions -693 to -679 [motif (ATTCT)8 ] 1 +140 +168 (CTGT)7 ]. Genetic diversity was measured as mean numbers alleles expected...
Sperm from the toad Bufo arenarum must penetrate egg jelly before reaching vitelline envelope (VE), where acrosome reaction is triggered. When coat removed, sperm still bind to VE, but acrosomal exocytosis not promoted. Our previous work demonstrated that diffusible substances of coat, termed "egg water" (EW), triggered capacitation-like changes in B. sperm, promoting acquisition a transient fertilizing capacity. In present work, we correlated this capacity with ability undergo reaction,...
Many fish species display compensatory growth (CG), a phenomenon by which fasted grow faster during refeeding. However, most studies use group-housed approach that could be problematic in social when interaction between individuals is not considered or eliminated. Additionally, the hormone (GH)/insulin-like factors' (IGF-1 and IGF-2) axis implicated postnatal vertebrates, but its relevance CG fully understood. Thus, aim of this work was to determine whether occurs fish, Cichlasoma dimerus,...
In order to improve the understanding of pejerrey Odontesthes bonariensis , growth hormone (Gh)‐insulin‐like factor‐1(Igf1) axis, O. receptor type 1 ( ghr1 ) and 2 ghr2 mRNA sequences were obtained. Both transcripts ubiquitously expressed except in kidney, encephalon anterior intestine. Alternative both receptors found muscle. Interestingly, two different with alternative polyadenylation APA sites located long 3′ untranslated region UTR‐APA also liver. Hepatic insulin‐like factor igf1...
L-HGP is a highly glycosylated protein from Bufo arenarum egg-jelly coat that diffuses into the surrounding medium when strings of oocytes are incubated in saline solutions. was purified egg water and estimated percentage L-HGP/total 30%. In present study we examine, by indirect immunofluorescence, effect on acrosome status homologous spermatozoa. A high (77%) sperm lost 10% Ringer solution buffered with 10 mM Tris-HCl, pH 7.6, during 60 min, condition resembles osmolarity. The addition to...
Abstract The vitelline envelope (VE) participates in sperm‐egg interactions during the first steps of fertilization. In Bufo arenarum , this is composed at least four glycoproteins, with molecular masses 120, 75, 41, and 38 kDa molar ratio 1:1.3:7.4:4.8, respectively. These components were isolated covalently coupled to silanized glass slides order study their sperm‐binding capacity. When considering glycoproteins egg‐envelope assuming that each protein monovalent for sperm, assay showed...
Background information . The role of the jelly coat that surrounds amphibian oocytes has been widely discussed, but is poorly understood. presence essential for fertilization. However, structure and function molecules comprise have not thoroughly documented. L‐HGP (low‐molecular‐mass highly glycosylated protein) a protein present in toad, Bufo arenarum , diffuses to surrounding media. L‐HGP, when purified from egg water, protects sperm acrosome breakdown induced by hypotonic solutions....
Growth hormone (GH) can be orally administrated to fish in order increase growth rates. Fish is characterized by the hyperplasia and hypertrophy of muscle fibre throughout adult life. In this respect, GH could affect directly indirectly (by metabolic factors) development fibres. Recombinant pejerrey (r-pjGH) was expressed Escherichia coli refolded a highly efficient batch dilution system, obtaining 0.1 g L−1 without protein precipitation during refolding procedure. Orally administered...