A5089038228- Innovative Microfluidic and Catalytic Techniques Innovation
- Fluorine in Organic Chemistry
- Microfluidic and Capillary Electrophoresis Applications
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Chemical Synthesis and Analysis
- Electrowetting and Microfluidic Technologies
- Herpesvirus Infections and Treatments
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Toxin Mechanisms and Immunotoxins
- Chemical Synthesis and Reactions
- Chemical synthesis and alkaloids
- Oxidative Organic Chemistry Reactions
- Catalytic Cross-Coupling Reactions
- RNA and protein synthesis mechanisms
- Asymmetric Hydrogenation and Catalysis
- Microbial Natural Products and Biosynthesis
- Asymmetric Synthesis and Catalysis
- Mass Spectrometry Techniques and Applications
- Hemophilia Treatment and Research
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Biotin and Related Studies
Multiple Sclerosis Society
2023
Sphere Medical (United Kingdom)
2020-2021
Babraham Institute
2016-2018
Webb Institute
2016-2018
University of Cambridge
2013
GlaxoSmithKline (United Kingdom)
2002-2009
University of Leicester
2004
University of Birmingham
2002
University of Hawaii System
2000
University of Pittsburgh
1993
We report a microfluidic droplet-based approach enabling the measurement of chemical reactions individual enzyme molecules and its application to single-molecule-counting immunoassay. A device is used generate manipulate <10 fL droplets at rates up 1.3 × 10(6) per second, about 2 orders magnitude faster than has previously been reported. The femtodroplets produced with this can be encapsulate single biomolecular complexes tagged reporter enzyme; their small volume enables fluorescent product...
The ICP4 protein of herpes simplex virus can either increase or decrease the rate transcription mediated by RNA polymerase II, depending on target promoter. interplay DNA-protein and protein-protein contacts determining function has yet to be characterized, consequently molecular mechanism which acts remains unclear. transactivate minimal promoters containing only TATA homologies, therefore it is reasonable hypothesize that works influencing TATA-dependent assembly general factors via...
One billion bacteria screened in picodroplets.
Charybdotoxin is a high-affinity specific inhibitor of the high-conductance Ca2+-activated K+ channel found in plasma membranes many vertebrate cell types.Using channels reconstituted into planar lipid bilayer as an assay, we have purified toxin from venom scorpion Leiurus quinquestriatus by two-step procedure involving chromatofocusing on SP-Sephadex, followed reversed-phase high-performance liquid chromatography.Charybdotoxin shown to be highly basic protein with mass 10 kDa.Under our...
High- and ultrahigh-throughput label-free sample analysis is required by many applications, extending from environmental monitoring to drug discovery industrial biotechnology. HTS methods predominantly are based on a targeted workflow, which can limit their scope. Mass spectrometry readily provides chemical identity abundance for complex mixtures, here, we use microdroplet generation microfluidics supply picoliter aliquots at rates up including 33 Hz. This demonstrated small molecules,...
The primary goal of bioprocess cell line development is to obtain high product yields from robustly growing and well-defined clonal lines in timelines measured weeks rather than months. Likewise, high-throughput screening B cells hybridomas required for most engineering workflows. A substantial bottleneck these processes detecting isolating rare with the characteristics. Traditionally, this was achieved by resource-intensive method limiting dilution cloning, more recently aided semiautomated...
Supramolecular self‐assembly offers routes to challenging architectures on the molecular and macroscopic scale. Coupled with microfluidics it has been used make microcapsules—where a 2D sheet is shaped in 3D, encapsulating volume within. In this paper, versatile methodology direct accumulation of capsule‐forming components droplet interface using electrostatic interactions described. approach, charged copolymers are selectively partitioned microdroplet by complementary surfactant for...
ts701 is a temperature-sensitive mutant of herpes simplex virus type 1 strain KOS induced by hydroxylamine mutagenesis (C.T. Chu, D. S. Parris, R. A. F. Dixon, E. Farber, and P. Schaffer, Virology 98:168-181, 1979). In the present study, mutation rendering temperature sensitive was mapped to coordinates 0.609 through 0.614 in UL region genome. At nonpermissive temperature, (i) failed induce synthesis viral DNA, (ii) exhibited dramatically reduced ability drive replication plasmid containing...
Microfluidic microdroplets have increasingly found application in biomolecular sensing as well nanomaterials growth. More recently the synthesis of plasmonic nanostructures has led to surface‐enhanced Raman spectroscopy (SERS)‐based applications. However, study nanoassembly previously been hindered by lack on‐chip characterization tools, particularly at early timescales. Enabled a refractive index matching microdroplet formulation, dark‐field is exploited directly track formation...
Micron-scale droplets isolated by an immiscible liquid can provide miniaturised reaction vessels which be manipulated in microfluidic networks, and has seen a rapid growth development. In many experiments, the precise volume of these microdroplets is critical parameter influenced external factors. this work, we demonstrate combination imaging-based feedback pressure driven pumping to accurately control size produced device. The use fast-response, pressure-driving pumps allows flow quickly...
Herpes simplex virus type 1 infected cell polypeptide 4 (HSV-1 ICP4) is a multifunctional phosphoprotein that essential for viral infection. It both repressor and an activator of gene expression depending upon the promoter. ICP4 represses transcription from its own In present study, we used general factors HeLa nuclear extracts, recombinant TATA binding protein (TBP) TFIIB, transcriptional Sp1 to reconstitute in vitro promoter examine effects purified on transcription. was able effectively...
4-Deoxy-4,4-difluoro-glycosides have been synthesised for the first time via a direct sequence involving ring-closing metathesis and indium-mediated difluoroallylation with 1-bromo-1,1-difluoropropene in water. Two protecting group strategies were explored, one to allow protection of primary C-6 hydroxyl throughout sequence, while second was intended deprotection after RCM before dihydroxylation. The benzyl ether could be used role, pivaloyl is effective second. Dihydroxylations highly...
Droplet microfluidics coupled with mass spectrometry is evolving for high throughput screening. A commercial solution that can readily interface to existing instrumentation would be highly desirable and allow the technology become accessible those without experience in droplet microfluidics. Here, we demonstrate a reinjection workflow infuse samples into three common spectrometers, each different electrospray ionisation (ESI) source configurations. The ease of chip-mass (MS) coupling...
The use of the pentafluorophenyl (PFP) group as a sulfonic acid protecting has allowed synthesis new biaryl- and heterobiaryl-PFP-sulfonate esters by Suzuki−Miyaura reaction. successful employment novel inorganic base, anhydrous sodium tetraborate, was crucial to give products in excellent yields. PFP-sulfonate ester been previously shown be an alternative sulfonyl chlorides sulfonamides.
Building block methodology from trifluoroethanol and ring-closing metathesis using a Fürstner modification of Grubbs' conditions allows the rapid synthesis novel difluorinated cyclooctenones.
Vero cells were biochemically transformed with the gene encoding ICP4 of herpes simplex virus type 2 (HSV-2). These used as permissive hosts to isolate and propagate HSV-2 mutants defective in this gene. Two mutants, designated hr259 hr79, isolated. Neither mutant grew nontransformed cells, but both near wild-type levels ICP4-expressing cells. contains a deletion about 0.6 kilobases which eliminates mRNA start site hr79 mutation maps by marker rescue portion carboxy-terminal half protein....
A (bromodifluoromethyl)alkyne has been deployed in a stereoselective route to difluorinated aldonic acid analogues, which Sharpless asymmetric dihydroxylation reaction and diastereoisomer separation set the stage for phenyl group oxidation.
Journal Article Solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine Get access Clive A. Smith, Smith 1 Department Biochemistry, University College and Middlesex School MedicineGower St. London WCIE 6BT, UK 1Present address: Cruachem Ltd, West Scotland Science Park, Acre, Road, Glasgow, Search for other works by this author on: Oxford Academic PubMed Google Scholar Yao-Zhong Xu, Xu Peter F. Swann Carcinogenesis, Volume 11, Issue 5, May 1990, Pages 811–816,...