A5059133010- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Advanced Electron Microscopy Techniques and Applications
- Cancer Research and Treatments
- Electron and X-Ray Spectroscopy Techniques
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- Polyomavirus and related diseases
McGill University
2018-2025
University of KwaZulu-Natal
2024
McGill University Health Centre
2023-2024
Max Planck Society
1994-2020
Max Planck Institute of Biochemistry
2016-2020
Weatherford College
2018
Harvard University
2011-2017
University of Otago
2015
Boston University
2014
Harvard University Press
2013-2014
Nuclear factor kappa B (NF-κB) has been implicated in the regulation of cell proliferation, transformation, and tumor development. We provide evidence for a direct link between NF-κB activity cycle regulation. was found to stimulate transcription cyclin D1, key regulator G1checkpoint control. Two binding sites human D1 promoter conferred activation by as well growth factors. Both levels kinetics expression during G1phase were controlled NF-κB. Moreover, inhibition caused pronounced reduction...
Journal Article Identification of differentially expressed mRNA species by an improved disply technique (DDRT-PCR) Get access David Bauer, Bauer 1Max-Plsnck-Gesellschaft and Humboldt University Search for other works this author on: Oxford Academic PubMed Google Scholar Heiko Muüller, Muüller Jens Reich, Reich 2Max Delbruück Center Molecualr MedicineRobert-Roössle-str. 10, O-1115 Berlin-Buch Germany Heidemarie Riedel, Riedel vibeke Ahrenkiel, Ahrenkiel 3Danish Cancer Soceity, Division cancer...
Cyclin D1 is a cell-cycle regulator essential for G1 phase progression and candidate proto-oncogene implicated in pathogenesis of several human tumour types, including breast carcinomas. In spite the accumulating genetic evidence, however, there are no data regarding abundance properties cyclin protein cancer. We now report aberrant nuclear overexpression/accumulation about half 170 primary carcinoma specimens analyzed by monoclonal antibody immunohistochemistry, indicating that frequency...
Viral vectors are the most efficient tools for gene delivery, and search tissue-specific infecting viruses is important development of in vivo therapy strategies. The baculovirus Autographa californica nuclear polyhedrosis virus widely used as a vector expression foreign genes insect cells, its host specificity supposed to be restricted arthropods. Here we demonstrate that recombinant A. efficiently taken up by human hepatocytes via an endosomal pathway. High-level reporter from heterologous...
We used electron cryotomography to study the molecular arrangement of large respiratory chain complexes in mitochondria from bovine heart, potato, and three types fungi. Long rows ATP synthase dimers were observed intact cristae membrane fragments all species that examined. The dimer found exclusively on tightly curved edges. distance between along varied, but within F(1) heads was constant. angle monomers 70° or above. Complex I appeared as L-shaped densities tomograms reconstituted...
Abstract Electrically conductive pili from Geobacter species, termed bacterial nanowires, are intensely studied for their biological significance and potential in the development of new materials. Using cryo-electron microscopy, we have characterized nanowires G. sulfurreducens preparations that composed solely head-to-tail stacked monomers six-heme C-type cytochrome OmcS. The unique fold OmcS — closely wrapped around a continuous stack hemes can serve as an uninterrupted path electron...
To elucidate the regulator-versus-target relationship in cyclin D1/cdk4/retinoblastoma protein (pRB) pathway, we examined fibroblasts from RB-1 gene-deficient and wild-type littermate mouse embryos (ME) human tumor cell lines that differed status of gene. The RB+/+ RB-/- ME expressed similar levels D-type cyclins, cdk4, cdk6, showed analogous spectra abundance cellular proteins complexed with cdk4 and/or cyclins D1 D2, exhibited comparable associated kinase activities. Of two established...
The cytomegalovirus (CMV) major immediate-early promoter/enhancer is active in many cell culture systems and considered to be one of the strongest promoters vitro. However, when this promoter was used vivo approaches gene therapy, it silenced within a few weeks several organs including liver. In study, we demonstrated transcriptional inactivation CMV mouse contrast promoter, hybrid consisting minimal enhancer II hepatitis B virus for at least 11 While investigating reason shutdown did not...
The retinoblastoma gene product (pRB) participates in the regulation of cell division cycle through complex formation with numerous cellular regulatory proteins including potentially oncogenic cyclin D1. Extending current view emerging functional interplay between pRB and D-type cyclins, we now report that D1 expression is positively regulated by pRB. Cyclin mRNA protein specifically downregulated cells expressing SV40 large T antigen, adenovirus E1A, papillomavirus E7/E6 oncogene products...
Conditional mutant mice equipped with heterologous recombination systems (Cre/lox or Flp/frt) are promising for studying tissue-specific gene function and designing better models of human diseases. The utility these depends on the cell target specificity, efficiency control over timing (in)activation. We have explored adenoviral vectors transgenic expressing Cre under promoters to achieve Cre/lox-mediated somatic LacZ reporter gene, using a newly generated flox mouse strain. When adeno...
Silicon-based MEMS techniques dominate sub-millimeter scale manufacturing, while a myriad of conventional methods exist to produce larger machines measured in centimeters and beyond. So-called mesoscale devices, existing between these length scales, remain difficult manufacture. We present versatile fabrication process, loosely based on printed circuit board manufacturing techniques, for creating monolithic, topologically complex, three-dimensional parallel at the millimeter centimeter...
Glucose homeostasis and growth essentially depend on the hormone insulin engaging its receptor. Despite biochemical structural advances, a fundamental contradiction has persisted in current understanding of ligand-receptor interactions. While biochemistry predicts two distinct binding sites, 1 2, recent analyses have resolved only site 1. Using combined approach cryo-EM atomistic molecular dynamics simulation, we present structure entire dimeric receptor ectodomain saturated with four...
Poliovirus infection is initiated by attachment to a receptor on the cell surface called Pvr or CD155. At physiological temperatures, catalyzes an irreversible expansion of virus form expanded capsid 135S particle. This results in externalization myristoylated protein VP4 and N-terminal extension VP1, both which become inserted into membrane. Structures forms poliovirus several related viruses have recently been reported. However, until now, it has unclear how binding triggers viral at...
Repression of genes by Polycomb requires that PRC2 modifies their chromatin trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure dinucleosomes reveals how binding its catalytic subunit EZH2 to nucleosomal DNA orients N-terminus via an extended network interactions place H3K27 into site. Unmodified occupies a critical position in EZH2-DNA interface. Mutation arginine or alanine inhibits...