A5028891323- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Plant Pathogens and Resistance
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Plant Parasitism and Resistance
- Plant Disease Management Techniques
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Banana Cultivation and Research
- Entomopathogenic Microorganisms in Pest Control
- Cassava research and cyanide
- Soybean genetics and cultivation
- Ginger and Zingiberaceae research
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Phytochemistry and biological activities of Ficus species
- Microbial Community Ecology and Physiology
University of Pretoria
2015-2024
Forestry and Agricultural Biotechnology Institute
2016
James Hutton Institute
2006
Type VI secretion systems (T6SSs) are a class of macromolecular machines that recognized as an important virulence mechanism in several gram-negative bacteria. The genome Pantoea ananatis LMG 2665(T), pathogen pineapple fruit and onion plants, carries two gene clusters whose predicted products have homology with T6SS-associated from other Nothing is known regarding the role these T6SS-1 T6SS-3 biology P. ananatis. Here, we present evidence plays pathogenicity 2665(T) while strain lacking...
The ability to adapt adverse conditions permits many bacterial species be virtually ubiquitous and survive in a variety of ecological niches. This is particular importance for plant pathogenic bacteria that should able exist, except their host plants, different environments e.g. soil, water, insect-vectors etc. Under some these conditions, encounter absence nutrients persist, acquiring new properties related resistance stress factors (cross-protection). Although studies describe the...
Long noncoding RNAs (lncRNAs) represent a class of RNA molecules that are implicated in regulation gene expression both mammals and plants. While much progress has been made determining the biological functions lncRNAs mammals, functional roles plants still poorly understood. Specifically, long intergenic nocoding (lincRNAs) plant defence responses yet to be fully explored.In this study, we used strand-specific sequencing identify 1113 lincRNAs potato (Solanum tuberosum) from stem tissues....
Summary Pectobacterium carotovorum ssp. brasiliense 1692 ( Pcb1692 ) is an important emerging pathogen of potatoes causing blackleg in the field and soft rot during post‐harvest storage. Blackleg diseases involve bacterial colonization vascular tissue formation aggregates, also known as biofilms. To understand role quorum sensing by , we generated a Δ expI mutant strain. Inactivation led to reduced production plant cell wall‐degrading enzymes (PCWDEs), inability produce acyl homoserine...
Unprecedented insights into the biology and functions of bacteria have been continue to be gained through studying bacterial secretion systems in isolation. This method, however, results our understanding being primarily based on idea that they operate independently, ignoring subtleties downstream interconnections. Gram-negative are naturally able adapt navigate their frequently varied dynamic surroundings, mostly because covert connections between systems. Therefore, comprehend some linked...
Abstract Background The Type VI secretion system (T6SS) has been identified in several different bacteria, including the plant pathogen Pantoea ananatis . Previous silico analyses described three T6SS loci present pathogenic strain of P. LMG 20103. This initial investigation extended to include an additional seven sequenced strains together with 39 from ecological niches. Comparative and phylogenetic were used investigate distribution, evolution, intra-strain variability operon structure...
Pectobacterium carotovorum subsp. brasiliense is a newly identified member of the potato soft rot enterobacteriaceae. The pathogenesis this pathogen still poorly understood. In study, an mCherry-P. brasiliense-tagged strain was generated to study P. brasiliense-potato plant interactions. Prior use, tagged evaluated for in vitro growth, plasmid stability, and virulence on tubers shown be similar wild type. Four cultivars were stem-based resistance against brasiliense. Confocal laser-scanning...
Soft-rot Enterobacteriaceae (SRE), typified by Pectobacterium and Dickeya genera, are phytopathogenic bacteria inflicting soft-rot disease in crops worldwide. By combining genomic information from 100 SRE with whole-transcriptome data sets, we identified novel transcriptional associations among key pathogenicity themes this group. Comparative genomics revealed solid linkage between the type I secretion system (T1SS) carotovoricin bacteriophage (Ctv) conserved 96.7% of genomes. Moreover,...
The complexity of plant microbial communities provides a rich model for investigating biochemical and regulatory strategies involved in interbacterial competition. Within these niches, the soft rot Enterobacteriaceae (SRE) represents an emerging group plant-pathogens causing rot/blackleg diseases resulting economic losses worldwide variety crops. A preliminary screening using next-generation sequencing 16S rRNA comparatively analyzing healthy diseased potato tubers, identified several taxa...
Phytopathogenic oomycetes are known to successfully infect their hosts due ability secrete effector proteins. Of interest many researchers effectors with the N-terminal RxLR motif (Arginine-any amino acid-Leucine-Arginine). Owing advances in genome sequencing, we can now comprehend high level of diversity among oomycete effectors, and similarly, conservation within species referred here as "core" (CREs). Currently, there is a considerable number CREs that have been identified oomycetes....
A molecular‐based assay was employed to analyse and accurately identify various root‐knot nematodes ( M eloidogyne spp.) parasitizing potatoes S olanum tuberosum ) in South Africa. Using the intergenic region IGS 28S D2–D3 expansion segments within ribosomal DNA (r ), together with between cytochrome oxidase subunit II COII 16S r RNA gene of mt , 78 composite potato tubers collected from seven major growing provinces were analysed all species present identified. During this study, ....