A5101837563- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Historical and Literary Studies
- Congenital limb and hand anomalies
- Renaissance Literature and Culture
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Historical and Literary Analyses
- Advanced biosensing and bioanalysis techniques
- Carcinogens and Genotoxicity Assessment
- French Literature and Criticism
- Cancer-related Molecular Pathways
- DNA and Nucleic Acid Chemistry
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Bioactive Compounds and Antitumor Agents
- Reconstructive Surgery and Microvascular Techniques
- Estrogen and related hormone effects
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
Leiden University
2023
Cardiff University
2011-2022
Institut de Recherche et d'Histoire des Textes
2022
Textron Systems (United Kingdom)
2022
University College London
1997-2017
Cancer Research UK
2009-2017
London Cancer
2017
The Portland Hospital
2012-2017
Great Ormond Street Hospital
1994-2015
Tenovus Cancer Care
2008-2015
Several genes conferring susceptibility to breast and ovarian cancer, notably BRCA1 BRCA2, have been identified. The majority of the familial aggregation cancer is, however, not explained by these genes. We previously derived, using segregation analysis, a model (BOADICEA, Breast Ovarian Analysis Disease Incidence Carrier Estimation Algorithm) in which is mutations BRCA2 together with polygenic component reflecting joint multiplicative effect multiple small on risk. Here, we consider...
Abstract The argyrophilic staining (AgNOR) technique, novel in histopathology, was applied to a series of 20 non‐Hodgkin's lymphomas (NHL) established Kiel subtype. method demonstrates nucleolar organizer regions (NORs) by virtue sulphydryl groups on their associated proteins and the enumeration AgNOR foci has been previously shown discriminate between NHL low‐ high‐grade histological types. This finding confirmed results were compared with those obtained means DNA flow cytometry performed...
A method for DNA histogram analysis is described that depends only on the simple assumption data are normally distributed and a requirement G1 peak present. probability density function was derived from extracted S-phase component whole histogram. The model tested with simulated data, good agreement between predicted known proportions in G1, S, + M found. Good also found duplicates of experimentally data. Some systematic errors present certain types histograms. However, these result small...
Macrophage (MØ) biology is routinely modelled in the peritoneal cavity, a vascular tissue readily infiltrated by leukocytes during inflammation. After several decades of study, no consensus has emerged regarding importance situ proliferation versus peripheral monocyte recruitment for maintenance resident MØs. By applying specific measures mitosis, we have monitored MØ newborn development, adulthood and acute resolving inflammation young adult mice. Despite nature ease leukocyte entry, MØs...
Introduction to the analysis of cross-over designs - basic principles and some useful tools Latin square 2-treatment, 2-period, 2-sequence design modifications with variance balance lacking categorical data from ordinary least squares estimation versus other criteria justification for using methodology presented in this book topics designs.
With increasing interest being shown in nucleolar organizer regions (NORs) pathology, it was considered of great importance to evaluate the effect some more commonly used and specialized fixatives on demonstration these moieties. NORs can be demonstrated paraffin sections by a silver technique (AgNOR method) which developed from method cytogeneticists for chromosome spreads. The degree staining is dependent fixation regime employed results may vary greatly one fixative another. schedules...
Abstract Using normal human fibroblasts we have determined the ability of far (254 nm), mid (310 nm) or near (365 UV radiation to: (i) induce pyrimidine dimers (detected as endonuclease sensitive sites) and DNA single‐strand breaks in alkali); (ii) elicit excision repair, monitored unscheduled synthesis (UDS); (iii) reduce colony‐forming ability. Unscheduled studies were also performed on dimer excision‐defective xeroderma pigmentosum (XP) cells, survival extended to include XP Bloom's...
Polysialic acid (polySia), an α-2,8-glycosidically linked polymer of sialic acid, is a developmentally regulated post-translational modification predominantly found on NCAM (neuronal cell adhesion molecule). Whilst high levels are expressed during development, peripheral adult organs do not express polySia-NCAM. However, tumours neural crest-origin re-express polySia-NCAM: its occurrence correlates with aggressive and invasive disease poor clinical prognosis in different cancer types,...
Using a newly developed, sensitive, and specific RIA, we measured the serum concentrations of inhibin, together with those FSH, LH, sex steroids, throughout puberty in 99 boys 102 girls attending suburban Melbourne school. Serum inhibin levels rose from geometric mean level 161 U/L (range, 87–310; 67% confidence interval) at stage I to 442 300–626) V, while corresponding values were 97 46–204) 231 187–372), respectively. strongly correlated age testosterone, estradiol; all hormones increased...
Single-stranded DNA (ssDNA)-binding protein (SSB) protects ssDNA from degradation and recruits other proteins for replication repair. Escherichia coli SSB is the prototypical eubacterial in a family of tetrameric SSBs. It consists structurally well-defined binding domain (OB-domain) disordered C-terminal (C-domain). The eight-residue segment (C-peptide) mediates to many different SSB-binding proteins. Previously published nuclear magnetic resonance (NMR) data monomeric state at pH 3.4 showed...
Current evidence suggests that the complex nature of mammalian chromatin can result in concealment DNA damage from repair enzymes and their co-factors. Recently it has been proposed acetylation histone proteins may provide a surveillance system whereby damaged regions become exposed due to changes accessibility. This hypothesis tested by: (i) using n-butyrate induce hyperacetylation human adenocarcinoma (HT29) cells; (ii) monitoring enzymatic accessibility permeabilised (iii) measuring u.v....