The biologically active components of the methanol extracts R. mucronata were identified using GC/MS. anticancer effects each extract from leaves and stem evaluated against cancer non-cancer cell lines. MTT assay was used in order to evaluate viability, IC50 selectivity indices calculated relation a positive control (doxorubicin). results showed that 11 8 different chemical compounds found stems mucronata, respectively. constituents had colon (CaCo-2), with levels 127 ± 4 μg/mL 107 6 μg/mL,...

Alzheimer's disease is regarded as a common neurodegenerative that may lead to dementia and the loss of memory. We report here nootropic anti-amnesic effects both peppermint rosemary oils using rat model scopolamine-induced amnesia-like AD. Rats were administered orally with two doses (50 100 mg/kg) each single oil combined oils. The positive group used donepezil (1 mg/kg). In therapeutic phase, rats scopolamine through oral administration During showed significant (p < 0.05) decrease in...

Anti-tumor compounds from natural products are being investigated as possible alternatives for cancer chemotherapeutics that have serious adverse effects and tumor resistance. Calystegia silvatica was collected the north coast of Egypt extracted via methanol n-hexane sub-fraction. The biologically active were identified extracts leaves stems plant using GC-MS HPLC. antitumor properties both parts against non-cancer cell lines MTT assay, IC50 in comparison to doxorubicin calculated. main...

Polysialic acid (polySia), an α-2,8-glycosidically linked polymer of sialic acid, is a developmentally regulated post-translational modification predominantly found on NCAM (neuronal cell adhesion molecule). Whilst high levels are expressed during development, peripheral adult organs do not express polySia-NCAM. However, tumours neural crest-origin re-express polySia-NCAM: its occurrence correlates with aggressive and invasive disease poor clinical prognosis in different cancer types,...

Tephorosia purpurea subsp. apollinea was extracted with methanol and n-hexane to obtain sub-fractions. The chemical compounds identified GC-MS HPLC in T. extracts showed antioxidant anticancer properties. activities were investigated using DDPH ABTS assays, MTT assay, respectively. Stigmasta-5,24(28)-dien-3-ol, (3 β,24Z)-, 9,12,15-octadecatrienoic acid methyl ester, phytol, chlorogenic acid, quercetin the major detected apollinea. These possessed extract properties radical scavenging of 84%...

Six novel ciprofloxacin-1,2,3-triazole hybrids (6a-f) were synthesized via click reaction, by reacting of methyl 1-cyclopropyl-6-fluoro-4-oxo-7-(4-(3-oxobutanoyl)piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylate (5) with various aryl azides (9a-f). The new compounds characterized using High-Resolution Mass Spectrometry (HRMS), 1H NMR, 13C and elemental analysis. Compounds screened for their in vitro anticancer activity against three cell lines, namely, non-small lung cancer (A549),...

Abstract Bladder cancer is the tenth most common worldwide, where its burden remains a challenge and needs new novel therapies. Several reports indicate expression of CYP4Z1 CYP1B1 in many tumours. Their expressions are associated with poor prognosis, therefore proposed as promising biomarkers or targets for anticancer therapy. By using immunohistochemistry, was evaluated panel different types bladder cancer, enzymes’ relation to histopathological features were assessed. Results showed an...

cervical cancer is one of the most common malignancies in women worldwide and its management remains challenging complex. As Cytochrome4Z1 (CYP4Z1) overexpressed many tumours, expression unknown. Therefore, present study aimed to evaluate CYP4Z1 cancers.CYP4Z1 was immunohistochemically assessed 100 cases cancers along with ten normal cervix tissues, enzyme's relationship several clinicopathological features survival explored.CYP4Z1 strongly expressed 55% patients. Normal samples were...

Background: A biofilm is group of bacteria embedded in a self-produced matrix made polysaccharide and protein.Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics disinfectant chemicals as well resisting phagocytosis.Biofilm much greater resistance than their free-living counterparts.Essential oils are cheap effective alternative that could be used combat resistant microorganisms.The aim the present study was determine effects essential oil wild...

Background Current standard treatments for patients with recurrent cervical cancer are not very effective and associated severe toxicity. Recently, the rational approach discovery of new therapies is based on alterations in molecular biology cells. One emerging changes cells aberrant expression cytochrome P450 1B1 (CYP1B1). This unique enzyme has been reported to be selectively overexpressed several cancers. Objective The aim this study was examine CYP1B1 cancers assess enzyme’s relationship...

Context: Euphorbia hierosolymitana is a member of species having restricted use in traditional medicine eastern Mediterranean countries. Aims: To phytochemically analyze different extracts and to investigate their anti-cancer activity against panel cancer cell lines. Methods: The aerial parts the plant were extracted by n-butanol ethyl acetate. Each extract was subjected Gas Chromatography-Mass Spectrometry (GC-MS) determine bioactive compounds. Additionally, each compared positive control...

Purpose: To evaluate the phytochemical profile of methanol extract Ephedra foeminea and assess its anti-carcer effect on a large set normal cancerous cell lines.&#x0D; Methods: Extraction air-dried powder aerial parts E. was carried out with methanol. The bioactive compounds in were determined using gas chromatography/mass spectrometry (GC-MS). anti-cancer by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay against various types cancer lines. Serial concentrations...

Background: There is a high variability in chemical composition of essential oil from Artemisia herba-alba grown different countries and localities the same country. This has led to characterization many oil-dependent chemotypes assigned plant. Only one report was published on Jordan. Aim: The current study aims determines wild south Jordan test their activity against clinical isolate antibiotics resistant bacteria. Methodology: oils were isolated by hydrodistillation analysed Gas...

Cytochromes P450 (CYPs) constitute an enzyme family involved in the oxidative metabolism of a wide variety endogenous and exogenous compounds, including anti-cancer drugs carcinogens. Unlike other human CYPs, CYP4Z1 is highly expressed breast carcinoma associated with poor prognosis. As result, was hypothesised to be potential biomarker or drug target for discovery development promising therapies.CYP4Z1 expression immunohistochemically studied set 100 different tissues, normal, benign,...

Colon cancer is a leading cause of mortality worldwide. It has relatively poor prognosis; therefore, new therapies are needed. One the tumour-related enzymes that gained considerable interest CYP4Z1. This enzyme been expressed in many tumours and hypothesized as potential biomarker or target for novel anticancer therapies.CYP4Z1 overexpression was immunohistochemically examined large panel colon tissue types including normal, benign, primary metastatic ones, enzyme's relation to...

Triple-negative breast cancer (TNBC) is characterized by high mortality rate, and its clinical management difficult complex. Therefore, there a need for extensive efforts aimed at accelerating the discovery of novel therapies TNBC. CYP4Z1 has been implicated in development cancer. The current study characterizing expression on TNBC.Using immunohistochemistry, was evaluated 122 TNBC samples, four samples cancers expressing ER, PR, HER-2, normal tissues. association between enzyme various...

Background and Objective: Ovarian cancer is a leading cause of death in females. Since its treatment challenging causes severe side effects, novel therapies are urgently needed. One the potential enzymes implicated progression cancers Cytochrome 4Z1 (CYP4Z1). Its expression ovarian remains unknown. Therefore, current study aims to assess CYP4Z1 different subtypes cancers. Materials Methods: Immunohistochemistry was used characterize 192 cases along with eight normal tissues. The enzyme’s...

Abstract The distinct conformational characteristics, functionality, affordability, low toxicity, and usefulness make calixarene-based compounds a promising treatment option for cancer. aim of the present study is to synthesize new compound assess its anticancer potential on some human cancer cells. synthesized C-4-Hydroxyphenylcalix[4] resorcinarene ( HPCR ) was characterized by several spectroscopic techniques such as 1HNMR, 13CNMR, X-ray crystallographic analysis confirm purity identity....

Treatment of metastatic breast cancer patients is challenging and remains a major underlying cause female mortality. Understanding molecular alterations in tumor development critical to identify novel biomarkers targets for diagnosis therapy. One the aberrant expressions gaining recent research interest glypican-1. Several studies reported strong glypican-1 expression various types human cancers. However, none these investigated large cohort histopathological subtypes.Immunohistochemistry...

This study aimed to see how effective Globularia arabica and Malva slyvestries-based cream formulations were at healing scald burn wounds in rats. Depending on ointment, preparations of 1%, 5%, 10% w/w created. For comparison, an ointment base a regular composed soframycine utilized. Rats introduced by solidifying equipment 100°C 14-mm2 shaved dorsal region. A deep second-degree was created, the percentage wound contraction measured over next 15 days. The rats euthanized days 8 15,...

Context: Microbial biofilms and antibiotic-resistant bacterial strains pose a challenge in clinical world as they fail to respond conventional treatments. This failure of antibiotic treatment led researchers look for alternatives. A possible alternative is plants derived essential oils. Many studies have reported that certain oils succeed where antibiotics fail. Aims: To test antibacterial antibiofilm activities oil Achillea biebersteinii its mode action. Methods: Minimum biofilm inhibitory...