Endoplasmic reticulum (ER) stress contributes to acute kidney injury induced by several causes. Kidney dysfunction was shown be influenced gender differences. In this study we observed differences in the severity of between male and female mice response tunicamycin, an ER agent. Tunicamycin-treated showed a severe decline function extensive damage proximal tubules outer cortex (S1 S2 segments). Interestingly, tunicamycin-treated did not show function, their kidneys localized primarily inner...

The minimal machinery mediating store operated Ca2+ entry (SOCE) include an ER sensor -STIM1- and a plasma membrane (PM) Ca2+-selective channel Orai1. Here we quantitatively dissect Orai1 trafficking dynamics show that recycles rapidly at the PM (Kex ≃ 0.1 min-1), with ∼40% of total pool localizing to steady state. A subset intracellular localizes sub-plasmalemal compartment. Store depletion is coupled enrichment in STIM1-dependent fashion. This due trapping into cortical STIM1 clusters...

We here characterize changes in metabolite patterns glioblastoma patients undergoing surgery and concurrent chemoradiation using machine learning (ML) algorithms to metabolic during different stages of the treatment protocol. examined 105 plasma specimens (before surgery, 2 days after surgical resection, before starting chemoradiation, immediately chemoradiation) from 36 with isocitrate dehydrogenase (IDH) wildtype glioblastoma. Untargeted GC-TOF mass spectrometry-based metabolomics was used...

Tissue fibrosis is a major cause of death in developed countries. It commonly occurs after either acute or chronic injury and affects diverse organs, including the heart, liver, lung, kidney. Using renal ablation model kidney disease, we previously found that development progressive was dependent on p21 WAF1/Cip1 expression; genetic knockout gene greatly alleviated this disease. In present study, expanded observation report induced by two different injuries to also p21. addition, when...

Vertebrate oocytes are maintained in meiotic arrest for prolonged periods of time before undergoing oocyte maturation preparation fertilization. Cyclic AMP (cAMP) signaling plays a crucial role maintaining arrest, which is released by species-specific hormonal signal. Evidence both frog and mouse argues that constitutively active G-protein coupled receptor (GPCR) leading to high cAMP levels. Because activated GPCRs typically targeted endocytosis as part the signal desensitization pathway, we...

Cisplatin cytotoxicity is dependent on cyclin-dependent kinase 2 (Cdk2) activity in vivo and vitro. We found that an 18-kDa protein identified by mass spectrometry as p21(WAF1/Cip1) was phosphorylated Cdk2 starting 12 h after cisplatin exposure. The analysis showed it at serine 78, a site not previously identified. adenoviral transduction of p21 before exposure protects from inhibiting Cdk2. Although causes induction endogenous p21, the protection inefficient. hypothesized phosphorylation 78...

Abstract Apoptosis is a regulated form of cell death that proceeds by defined biochemical pathways. Most apoptosis controlled interactions between pro-survival and pro-apoptotic Bcl-2 family proteins in which often the consequence permeabilization mitochondrial outer membrane. Many drugs affect this equilibrium to favor but process not completely understood. We show chemotherapeutic drug cisplatin initiates an apoptotic pathway phosphorylation member, Bcl-xL, cyclin-dependent kinase 2. The...

The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 (mPRs) mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show the topology of mPRβ is similar to adiponectin and opposite G-protein-coupled (GPCRs). Using Xenopus oocyte meiosis as a well-established readout signaling, demonstrate requires adaptor protein APPL1 kinase Akt2. We further induces clathrin-dependent...

In medical and health sciences, the detection of cell injury plays an important role in diagnosis, personal treatment disease prevention. Despite recent advancements tools methods for image classification, it is challenging to classify images with higher precision accuracy. Cell classification based on computer vision offers significant benefits biomedicine healthcare. There have been studies reported where techniques complemented by Artificial Intelligence-based classifiers such as...

Ca(2+)-activated Cl(-) channels (CaCCs) play important physiological functions in epithelia and other tissues. In frog oocytes the CaCC Ano1 regulates resting membrane potential block to polyspermy. Here, we show that expression increases oocyte surface, revealing a novel function for regulating cell morphology. Confocal imaging shows microvilli length, which requires ERM-protein-dependent linkage cytoskeleton. A dominant-negative form of ERM protein moesin precludes Ano1-dependent increase...

A chaperone complex (CCT) regulates the trafficking of a calcium channel (Orai1) and, as such, modulates cellular function.

In contrast to the well-defined role of Ca2+ signals during mitosis, contribution signaling meiosis progression is controversial, despite several decades investigating and its effectors in vertebrate oocyte maturation. We have previously shown that Xenopus maturation, are dispensable for entry into germinal vesicle breakdown. However, normal homeostasis essential completion I extrusion first polar body. this study, we test downstream mediating effects show calmodulin...

The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 (mPRs) mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show the topology of mPRβ is similar to adiponectin and opposite G-protein-coupled (GPCRs). Using Xenopus oocyte meiosis as a well-established readout signaling, demonstrate requires adaptor protein APPL1 kinase Akt2. We further induces clathrin-dependent...

Targeting cell cycle and inducing DNA damage by activating death pathways are considered as effective therapeutic strategy for combating breast cancer progression. Many of the naturally known small molecules target these signaling against resistant and/or aggressive types cancers. Here, we investigated effect catechol, a occurring plant compound, its specificity chemotherapeutic efficacies in (MCF‐7 MDA‐MB‐231) cells. Catechol treatment showed concentration‐dependent cytotoxicity...

ABSTRACT Progesterone mediates its physiological functions through activation of both transcription-coupled nuclear receptors and seven-pass-transmembrane progesterone (mPRs), which transduce the rapid non-genomic actions by coupling to various signaling modules. However, immediate mechanisms action downstream mPRs remain in question. Herein, we use an untargeted quantitative proteomics approach identify mPR interactors better define signaling. Surprisingly, very-low-density lipoprotein...

Cisplatin cytotoxicity is dependent on cyclin-dependent kinase 2 (Cdk2) activity in vivo and vitro. A Cdk2 mutant (Cdk2-F80G) was designed which the ATP-binding pocket altered. When expressed mouse kidney cells, this protein inactive, did not inhibit endogenous Cdk2, but protected from cisplatin. The localized cytoplasm, when coexpressed with cyclin A, it activated, to nucleus, no longer cisplatin cytotoxicity. Cells exposed presence of activated had an apoptotic phenotype, endonuclease G...