We have tested the T helper cell (TH) potential of asymptomatic, HIV seropositive (HIV+) patients, using an in vitro assay for IL-2 production. Peripheral blood leukocytes (PBL) from 74 HIV+ patients and 70 HIV- control donors were TH function when stimulated with influenza A virus (FLU), tetanus toxoid (TET), HLA alloantigens (ALLO), or PHA. Of four different response patterns observed: (a) who responded to all stimuli (16%); (b) selectively unresponsive FLU TET, but ALLO PHA (54%); (c)...

Peripheral blood mononuclear cells (PBMCs) from many asymptomatic individuals infected with human immunodeficiency virus-type 1 (HIV) are unresponsive as measured by in vitro T cell proliferation and interleukin-2 (IL-2) production to influenza virus synthetic peptides of HIV envelope (Env). Strong virus- Env-stimulated IL-2 responses were restored when cultures stimulated the presence IL-12. Interferon-gamma PBMCs seropositive (HIV+) patients was also Furthermore, antigen-specific HIV-...

•Healthcare workers (HCW) are part of the frontline in struggle against pandemic.•Many HCWs have been infected with SARS-CoV-2 and ldied worldwide during pandemic.•We performed an international survey requested total numbers HCW deaths infections.•Data were obtained from 37 countries, where lost their lives substantial numbers.•We call on WHO Director-General to highlight this tragedy at COVID-19 website regularly. The coronavirus disease-2019 (COVID-19) pandemic is ongoing devastating...

The international response to the evolving Ebola epidemic in eastern Democratic Republic of Congo (DRC) has had interim successes while facing ongoing difficulties. outbreak occurred an area intractable conflict among multiple armed groups at a time contentious national elections. Despite porous borders and considerable population movement, however, transmission been confined North Kivu Ituri provinces. Factors potentially contributing this containment include conduct about 55 million...

Journal Article Relapsing Illness Due to Rochalimaea henselae in Immunocompetent Hosts: Implication for Therapy and New Epidemiological Associations Get access Daniel Lucey, Lucey From the Departments of Medicine Pathology, Wilford Hall U.S. Air Force Medical Center, Lackland Base, Texas; Analytical Chemistry Laboratory Special Bacterial Reference Laboratory, Meningitis Pathogens Branch, Division Mycotic Diseases, Center Infectious Centers Disease Control, Atlanta, Georgia; Roche Molecular...

Human eosinophils are known to lose Ia antigen expression as they mature, and, accordingly, obtained from the blood of five eosinophilic donors and three four normal failed display major histocompatibility complex class II HLA-DR, determined by flow cytometry. However, when these nine were maintained in culture with recombinant human granulocyte-macrophage colony-stimulating factor murine 3T3 fibroblasts, HLA-DR consistently developed on eosinophils. By days 4-6 culture, 24-97% HLA-DR+,...

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ABSTRACT A rapid (time to completion, <4 h, including DNA extraction) and quantitative touch-down (QTD) real-time diagnostic Pneumocystis carinii PCR assay with an associated internal control was developed, using fluorescence resonance energy transfer (FRET) probes for detection. The procedure significantly increased the sensitivity of compared a non-touch-down procedure. Tenfold serial dilutions cloned target were used as standards quantification. P. has been detected in respiratory...

Four synthetic peptides corresponding to the IIIB sequence of gp160 HIV were recently reported stimulate Th cell function by PBL from HIV-infected, asymptomatic patients. In present report, we used these same demonstrate CTL activity in a similar patient population. EBV-transformed B-cell lines asymptomatic, seropositive and seronegative control donors pre-incubated with peptides. Fresh 19 (76%) 25 lysed autologous targets pulsed at least one four Autologous two non-immunogenic not lysed....

The CD4 glycoprotein, expressed on leukocytes belonging to subsets of T lymphocytes and cells monocyte/macrophage lineage, participates in the functioning serves as a receptor for HIV-1 HIV-2. Human eosinophils, class granulocytic leukocytes, have been found express CD4. With anti-CD4 mAbs was demonstrable eosinophils from both normal eosinophilic donors. Eosinophils synthesized 55-kD polypeptide immunoprecipitable with two mAbs. Eosinophil bound gp120 assessed by competition anti-OKT4A, but...

To make synthetic peptide vaccines effective in a broad population of outbred humans, one would have to incorporate enough antigenic determinants elicit recognition by T cells most HLA types. We previously defined multideterminant regions the human immunodeficiency virus (HIV) envelope that include overlapping seen proliferating three or four haplotypes mice. now tested hypothesis peptides encompassing such will be recognized multiple murine MHC types as well representing Six 20-33 residues...

Abstract APC dysfunction may be important in immune dysregulation associated with HIV disease. Langerhans cells, epidermal APC, can infected HIV, although their function HIV-infected persons has not been studied. Therefore, we studied the immunologic of parallel blood (enriched for monocytes/macrophages (M phi) function, 21 HIV-seropositive (HIV+) and HIV-seronegative volunteers, including three monozygotic twin pairs discordant serology. cells from HIV+ patients were quantitatively normal...

Strongyloides hyperinfection syndrome may be complicated by paralytic ileus that interferes with the absorption of oral anti-helminthics. We report on administration ivermectin as a rectal enema preparation to renal transplant recipient and progressive ileus. Attempts at treatment using nasogastric albendazole were unsuccessful despite clamping tube after drug administration. Ivermectin tablets ground powder, resuspended in commercially available suspending agent, administered per rectum....

As of January 1990, 933 persons with human immunodeficiency virus type 1 (HIV-l) infection were clinically evaluated at Wilford Hall US Air Force (USAF) Medical Center. The Walter Reed HIV staging system was used in these evaluations to describe disease status and progression. Most diagnosed through mandatory testing the USAF asymptomatic time diagnosis. May 161 HIV-positive seroconverters (estimated overall seroconversion rate 0.156/1000 person-years between 30 June 1988 July 1990) had been...

T-cell-mediated cytotoxicity may play an important role in control of infection by the human immunodeficiency virus (HIV). In this study, we have identified and characterized a relatively conserved epitope HIV-1 reverse transcriptase recognized murine cytotoxic T cells. This was using antigen-specific CD8+ class I major histocompatibility complex-restricted T-cell (CTL) line, transfected fibroblast cell line expressing pol gene, recombinant vaccinia viruses containing different truncated...

Our website uses cookies to enhance your experience. By continuing use our site, or clicking "Continue," you are agreeing Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications Network Open Cardiology Dermatology Health Forum Internal Medicine Neurology Oncology Ophthalmology Otolaryngology–Head & Neck Surgery Pediatrics Psychiatry Archives of (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning /...