Abstract Hemotrophic mycoplasmas infect a variety of mammals. Although infection in humans is rarely reported, an association with immunocompromised state has been suggested. We report case Mycoplasma haemofelis–like HIV-positive patient co-infected Bartonella henselae.

The nucleoprotein (NP) is a vital target for the heterosubtypic immunity of CD8+ cytotoxic T lymphocytes (CTLs) due to its conservation among influenza virus subtypes. To further enhance cell NP, autophagy-inducing peptide C5 (AIP-C5) from CFP10 protein Mycobacterium tuberculosis was used. Mice were immunized intranasally (i.n.) with human adenoviral vectors, HAd-C5-NP(H7N9) or HAd-NP(H7N9), expressing NP an H7N9 without AIP-C5, respectively. Both vaccines developed similar levels...

Use of tumor-suppressive microRNAs (miRNAs) as anti-cancer agents is hindered by the lack effective delivery vehicles, entrapment miRNA within endocytic compartments, and rapid degradation nucleases. To address these issues, we developed a strategy that includes (1) targeting ligand, (2) an endosomal escape agent, nigericin (3) chemically modified miRNA. The DUPA (2-[3-(1,3-dicarboxy propyl) ureido] pentanedioic acid), was selected based on its specificity for prostate-specific membrane...

Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, objective herein was to use whole genome sequencing analysis M. suis define pathogenicity biochemical pathways. harvested from blood an experimentally infected pig. Following DNA extraction construction a paired end library, whole-genome performed using GS-FLX (454) Titanium chemistry. Reads on paired-end constructs were...

Rickettsia bacteria are responsible for diseases in humans and animals around the world, however few details available regarding its ecology circulation among wild human populations at high transmission risk Brazil. The aim of this study was to investigate occurrence ticks spp. boars, corresponding hunting dogs hunters.

Leptospirosis is considered a neglected zoonosis associated with infrastructure problems and low socioeconomic status, particularly slums. Since the disease mainly transmitted in urban settings by rat urine, this risk factor may be important predictor tool for prompt control effective prevention at local level endemic areas. Accordingly, present study aimed to propose an early spatial human leptospirosis settings, test methodology of molecular methods assessing Leptospira spp. trapped rats,...

Abstract OBJECTIVE To determine if oxidative stress induces phosphatidylserine (PS) externalization in canine erythrocytes and exposure to antioxidants prevents such changes. METHODS This was an vitro, experimental study using 5 healthy, adult, purpose-bred research Beagles. Fresh EDTA-anticoagulated blood samples were collected from each dog, harvested. For objective 1, exposed the pro-oxidant agents tert-butyl hydroperoxide (TBHP) at 2, 3, or 4 mM 2,2′-azobis(2-amidinopropane)...

Indigenous communities are reportedly among the most vulnerable populations exposed to rabies worldwide. Accordingly, this study aimed assess serum titers from healthy individuals, their dogs, and healthcare professionals of four São Paulo state, southeastern Brazil. Blood samples were collected, an epidemiological questionnaire applied. The processed by Fluorescent Antibody Virus Neutralization (FAVN) method. Overall, 35/299 (11.7%) individuals 22/166 (13.2%) dogs without prior vaccination...

Although hemoplasma infection has been widely described in animals, a few studies have conducted involving human populations, mostly as case reports. This was cross-sectional epidemiological study that accessed individuals and dogs from ten Indigenous communities of southern southeastern Brazil. A total 23/644 (3.6%) tested positive to hemoplasmas by quantitative polymerase chain reaction (qPCR) (cycle threshold; Ct ≤ 34.4), with 3/644 (0.5%) Mycoplasma haemocanis detection. In addition,...

Abstract Introduction: Diffuse large B-cell lymphoma (DLBCL) is a common and aggressive form of in dogs, closely resembling human DLBCL its clinical behavior molecular features. While CHOP chemotherapy the standard treatment, many dogs experience poor responses or early relapse, underscoring need for more comprehensive understanding disease’s underpinnings. This study aimed to investigate gene expression profiles canine identify potential biomarkers therapeutic targets. Materials Methods:...

Abstract Prostate cancer is a leading cause of cancer-related mortality in men. Current therapeutic options include surgical intervention, chemotherapy, immunotherapy, and localized or systemic radiation therapy. However, advanced metastatic prostate cancers are challenging to treat, underscoring the critical need for more effective strategies. Prostate-specific membrane antigen (PSMA), transmembrane glycoprotein, overexpressed up 1000-fold ∼90% cancers. The high expression level PSMA,...

Background: ‘ Candidatus Mycoplasma turicensis’ ( C Mtc) is a hemotrophic bacterial species that can, alone or in combination, induce anemia cats. The diagnostic test of choice for hemoplasma infections PCR. Conventional PCR assays have been developed the detection haemofelis Mhf ) and M. haemominutum’ Mhm) but not Mtc. Although real‐time reported all feline hemoplasmas, expense necessary instrumentation precludes its use Brazil many other countries. Objectives: goals this study were to...

Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. infects populations of free-ranging deer Brazil. Buffy coat samples from 64 Blastocerus dichotomus Porto Primavera, 18 Ozotocerus bezoarticus Pantanal, 21 O. Emas National Park were tested. Using PCR protocol amplify extracted DNA, 46/64 (72%) 10/18 (56%) 4/21 (19%) positive, giving an overall positive rate 58% for these wild deer. Sequencing phylogenetic analysis the...

The aims of this study were to determine the occurrence Anaplasma platys and Ehrlichia canis infection in dogs Porto Alegre, Southern Brazil; investigate their association with hematological abnormalities. Serum samples from 196 first tested using dot-ELISA for antibodies against spp. canis. Peripheral blood 199 subjected 16S rRNA nested PCR (nPCR) A. E. canis, followed by DNA sequencing ensure pathogen identity. A total 19/196 (9.69%) positive ELISA 28/199 (14.07%) PCR. All dog negative...

SUMMARY The aims of this study were to determine the prevalence hemoplasmas in a rural Brazilian settlement's population human beings, their dogs and horses, highly exposed tick bites; identify species parasitizing analyze factors associated with infection. Blood samples from 132 dogs, 16 horses 100 humans screened using pan-hemoplasma SYBR green real-time PCR assay followed by species-specific TaqMan PCR. A total 59/132 (44.7%) dog positive for (21 Mycoplasma haemocanisalone, 12 '...

Three hemoplasma species are recognized in domestic cats: Mycoplasma haemofelis, ‘Candidatus haemominutum’ and turicensis’. We report the prevalence hematological abnormalities of infection 369 cats from three different populations (blood donors, hospitalized shelter cats) Southern Brazil. Complete blood counts were performed at time collection, DNA was extracted tested by conventional PCR for each species. A total 79 samples (21.40%) positive least one The most prevalent haemominutum’, with...

Indigenous health has posted complex challenges worldwide, particularly due to historical economic, territorial, social and environmental processes, which may lead emergence reemergence of pathogens. In addition few Coxiella burnetii serosurveys in vulnerable populations, especially developing tropical countries, no comprehensive One Health approach focused on human-animal infection along with potential determinants. Accordingly, this study aimed assess the seroprevalence anti-C. antibodies...

The prevalence of obesity is increasing in dogs as well humans. C-reactive protein (CRP) an important tool for the detection inflammation and/or early tissue damage and linked to objective present study was determine if serum CRP levels are altered obese dogs. Fifteen lean (control group) 16 overweight (obese were examined. Blood samples collected under fasted conditions determination CRP, glucose, insulin, cholesterol, triglyceride, fructosamine. Results indicated that insulin resistant...

In the last few years, use of laparoscopy in veterinary medicine has expanded and consequently so was need for studies that establish advantages, disadvantages possible complications each procedure. The purpose current study to describe a laparoscopic splenectomy technique alterations due this access, compare it open procedure dogs. A total 15 healthy female mongrel dogs were used, with mean weight 17.4±2.5kg. animals distributed into three groups: Group IA (laparotomy) using double ligation...