A5088049090- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Neuroscience and Neural Engineering
- Cancer, Stress, Anesthesia, and Immune Response
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Pancreatic and Hepatic Oncology Research
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Olfactory and Sensory Function Studies
- Sleep and Wakefulness Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Bone Tissue Engineering Materials
- Neuroscience and Neuropharmacology Research
- 3D Printing in Biomedical Research
- Tendon Structure and Treatment
- Nanoparticle-Based Drug Delivery
- Sports injuries and prevention
- Spaceflight effects on biology
- melanin and skin pigmentation
- Nanoplatforms for cancer theranostics
- Cancer Genomics and Diagnostics
University of Oxford
2016-2025
Florida College
2022-2025
University of Florida
2022-2025
Canadian Sleep & Circadian Network
2018
Neuroscience Institute
2018
National Institute for Health Research
2017
NIHR Biomedical Research Centre at The Royal Marsden and the ICR
2017
John Radcliffe Hospital
2010-2016
Roche (Switzerland)
2015
Keele University
2003-2007
Objectives Black/African American (B/AA) pancreatic ductal adenocarcinoma (PDAC) patients have worse clinical outcomes than White and are underrepresented in genomic databases. We aimed to expand our understanding of the PDAC somatic landscape from a diverse cohort. Materials Methods Formalin-fixed paraffin-embedded specimens 24 surgically resected cases were collected, with self-reported race/ethnicity. Whole exome sequencing was performed on malignant benign tissue. Bioinformatics analysis...
This study reports the preliminary development of a novel magnetic particle-based technique that permits application highly localized mechanical forces directly to specific regions an ion-channel structure. We demonstrate this approach can be used and selectively activate mechanosensitive ion channel interest, namely TREK-1. It is shown manipulation particles targeted against extended extracellular loop region TREK-1 leads changes in whole-cell currents consistent with activity. Responses...
Light plays a critical role in the regulation of numerous aspects physiology and behaviour, including entrainment circadian rhythms sleep. These responses involve melanopsin (OPN4)-expressing photosensitive retinal ganglion cells (pRGCs) addition to rods cones. Nocturnal light exposure rodents has been shown result rapid sleep induction, which key role. However, studies have also that can elevated corticosterone, response is not compatible with To investigate these contradictory findings...
Melanopsin-expressing photosensitive retinal ganglion cells (pRGCs) represent a third class of photoreceptor [1Hankins M.W. Peirson S.N. Foster R.G. Melanopsin: an exciting photopigment.Trends Neurosci. 2008; 31: 27-36Abstract Full Text PDF PubMed Scopus (305) Google Scholar, 2Do M.T. Yau K.W. Intrinsically cells.Physiol. Rev. 2010; 90: 1547-1581Crossref (280) 3Hughes S. Hankins Melanopsin phototransduction: slowly emerging from the dark.Prog. Brain Res. 2012; 199: 19-40Crossref (68)...
Optogenetic strategies to restore vision in patients who are blind from end-stage retinal degenerations aim render remaining cells light sensitive once photoreceptors lost. Here, we assessed long-term functional outcomes following subretinal delivery of the human melanopsin gene (OPN4) rd1 mouse model degeneration using an adeno-associated viral vector. Ectopic expression OPN4 a ubiquitous promoter resulted cellular depolarization and ganglion cell action potential firing. Restoration pupil...
Melanopsin is the photopigment that confers photosensitivity to a subset of retinal ganglion cells (pRGCs) regulate many non-image-forming tasks such as detection light for circadian entrainment. Recent studies have begun subdivide pRGCs on basis morphology and function, but origin these differences not yet fully understood. Here we report identification two isoforms melanopsin from mouse Opn4 locus, previously described long isoform (Opn4L) novel short (Opn4S) more closely resembles...
Abstract TRPM1 is a spontaneously active non‐selective cation channel that has recently been shown to play an important role in the depolarizing light responses of ON bipolar cells. Consistent with this role, mutations gene have identified as principal cause congenital stationary night blindness. However, previous microarray studies Trpm1 and Trpm3 are acutely regulated by eyes mice lacking rods cones ( rd/rd cl ), finding consistent non‐image‐forming photoreception. In study we show...
Abstract A possible etiological factor for the social disability described in children with attention‐deficit hyperactivity disorder (ADHD) is a deficit ability to accurately evaluate emotional stimuli. Children ADHD were compared normal controls on battery of processing. This battery, Minnesota Tests Affective Processing (MNTAP), measures face perception and recognition affective stimuli as conveyed via facial expression, language, speech prosody. Overall, found be no different from their...
A vaccinia virus open reading frame (ORF) previously predicted to encode thymidylate kinase (TmpK) is shown an active enzyme.A copy of the ORF, generated by polymerase chain reaction, was cloned into Escherichia coli inducible expression vector.Cell extracts E. expressing gene contained high levels TmpK activity, whereas cells without did not.The ORF expressed from a yeast vector complemented Saccharomyces cerevisiae cdc8 mutant, demonstrating functional compatibility and enzymes.The be...
Melanopsin (OPN4) is a retinal photopigment that mediates wide range of non-image-forming (NIF) responses to light [1Freedman M.S. Lucas R.J. Soni B. von Schantz M. Muñoz David-Gray Z. Foster R. Regulation mammalian circadian behavior by non-rod, non-cone, ocular photoreceptors.Science. 1999; 284: 502-504Crossref PubMed Scopus (644) Google Scholar, 2Hattar S. Mrosovsky N. Thompson Douglas R.H. Hankins M.W. Lem J. Biel Hofmann F. R.G. Yau K.W. and rod-cone photoreceptive systems account for...
Abstract Gnat −/− , Cnga3 Opn4 triple knockout (TKO) mice lack essential components of phototransduction signalling pathways present in rods, cones and photosensitive retinal ganglion cells (pRGCs) are therefore expected to all sensitivity light. However, a number studies have shown that light responses persist these mice. In this study we use multielectrode array (MEA) recordings light-induced c-fos expression further characterise the TKO retina. Small, but robust electroretinogram type...
Retinal bipolar cells survive even in the later stages of inherited retinal degenerations (IRDs) and so are attractive targets for optogenetic approaches to vision restoration. However, it is not known what extent remodelling that these undergo during degeneration affects their function. Specifically, unclear if they free from metabolic stress, receptive adeno-associated viral vectors, suitable opsin-based tools able propagate signals by releasing neurotransmitter. Fluorescence activated...
During inherited retinal degenerations (IRDs), vision is lost due to photoreceptor cell death; however, a range of optogenetic tools have been shown restore light responses in animal models. Restored response characteristics vary between and the neuronal population which they are delivered: interplay these complex, but targeting upstream neurons (such as bipolar cells) may provide functional benefit by retaining intraretinal signal processing. In this study, our aim was compare two tools:...
ABSTRACT In order to gain a better understanding of the molecular epidemiology Mycobacterium bovis isolates in Cameroon, 75 M. collected three provinces northern Cameroon were studied by spoligotyping. For 65 these isolates, typing was also carried out pulsed-field gel electrophoresis (PFGE) with Dra I, and 18 typed restriction fragment length polymorphism (RFLP) analysis probe IS 6110 -RHS. Molecular techniques revealed high degree homogeneity, 10 spoligotypes for four PFGE profiles RFLP...
Melanopsin expressing photosensitive retinal ganglion cells (pRGCs) represent a third class of ocular photoreceptors and mediate range non-image forming responses to light. is G protein coupled receptor (GPCR) existing data suggest that it employs membrane bound signalling cascade involving Gnaq/11 type proteins. However, date the precise identity Gα subunits involved in melanopsin phototransduction remains poorly defined. Here we show Gnaq, Gna11 Gna14 are highly co-expressed pRGCs mouse...